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DOI: 10.1055/s-2002-25758
Oligonucleotide Arrays from Aldehyde-Bearing Glass with Coated Background
Publication History
Publication Date:
26 April 2002 (online)
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Abstract
Presented here is a method for preparing small DNA arrays on aldehyde-bearing glass slides. Immobilization involves reductive amination and employs oligonucleotides with 3′-terminal lysine residues, obtained in high yield from solid phase syntheses. Spot patterns are produced by protecting selected areas of the aldehyde slides with wax, coating the free surface with a methyl triethylene glycol derivative, and removing the wax with dichloromethane. The DNA arrays give better signal to noise ratios in hybridization experiments than slides without passified background.
Key words
oligonucleotides - Schiff bases - solid phase synthesis - DNA - molecular recognition
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References
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