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Planta Med 2002; 68(1): 60-63
DOI: 10.1055/s-2002-19875
Original Paper
Analysis
© Georg Thieme Verlag Stuttgart · New York

DNA Fingerprinting of Cannabis sativa Using Inter-Simple Sequence Repeat (ISSR) Amplification

Mareshige Kojoma1, 4 , Osamu Iida1 , Yukiko Makino2 , Setsuko Sekita3 , Motoyoshi Satake3
  • 1Izu Experimental Station for Medicinal Plants, National Institute of Health Sciences, Japan
  • 2Kanto-Shin'etsu Regional Narcotic Control Office, Japan
  • 3Division of Pharmacognosy and Phytochemistry, National Institute of Health Sciences, Tokyo, Japan
  • 4Plant Functions Laboratory, RIKEN (The Institute of Physical and Chemical Research), Saitama, Japan
Weitere Informationen

Publikationsverlauf

January 19, 2001

June 9, 2001

Publikationsdatum:
31. Januar 2002 (online)

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Abstract

Chemical analysis of cannabinoid, and Inter-Simple Sequence Repeat (ISSR) fingerprinting of DNA were used to identify different samples of Cannabis sativa L. for forensic purposes. Three samples were classified into two types, tetrahydrocannabinol (THC) and cannabidiol (CBD) chemo-types, by high performance liquid chromatography (HPLC). The two samples of the CBD type were not distinguished by their HPLC patterns. ISSR fingerprinting identified polymorphic DNA patterns between these samples. ISSR fingerprinting clearly differentiated between cannabis samples that could not be achieved by HPLC analysis.

Key words

Cannabis sativa - hemp - Cannabaceae - DNA profiling - ISSR - cannabinoid - HPLC

References

  • 1 Quimby M W, Doorenbos N J, Turner C E, Masoud A. Mississippi-grown marihuana-Cannabis sativa cultivation and observed morphological variations.  Economic Botany. 1973;  27 117-27
    PubMedGoogle Scholar
  • 2 Small E, Beckstead H D, Chan A. The evolution of cannabinoid phenotypes in Cannabis .  Economic Botany. 1975;  29 219-32
    PubMedGoogle Scholar
  • 3 Small E, Cronquist A. A practical and natural taxonomy for Cannabis .  Taxon. 1976;  25 405-35
    PubMedGoogle Scholar
  • 4 Fetterman P S, Keith E S, Waller C W, Guerrero O, Doorenbos N J, Qinby M W. Mississippi-grown Cannabis sativa L.: preliminary observation on chemical definition of phenotype and variations in tetrahydrocannabinol content versus age, sex, and plant part.  Journal of Pharmaceutical Sciences. 1971;  60 1246-9
    PubMedGoogle Scholar
  • 5 Rowan M G, Fairbairn J W. Cannabinoid patterns in seedling of Cannabis sativa L. and their use in the determination of chemical race.  Journal of Pharmaceutical Pharmacology. 1977;  29 491-4
    PubMedGoogle Scholar
  • 6 Baker P B, Gough T A, Taylor B J. The physical and chemical features of Cannabis plants grown in the United Kingdom of Great Britain and Northern Ireland from seeds of known origin.  Bulletin on Narcotics. 1982;  34 27-36
    PubMedGoogle Scholar
  • 7 Gillian R, Cole M D, Linacre A, Thorpe J W, Watson N D. Comparison of Cannabis sativa by random amplification of polymorphic DNA (RAPD) and HPLC of cannabinoid: a preliminary study.  Science & Justice. 1995;  35 169-77
    PubMedGoogle Scholar
  • 8 Jagadish V, Robertson J, Gibbs A. RAPD analysis distinguished Cannabis sativa samples from different sources.  Forensic Science International. 1996;  79 113-21
    CrossrefPubMedGoogle Scholar
  • 9 Gigliano G S, Finizio A D, Caputo P, Cozzolino S. Cannabis fingerprints by using Random Amplified Polymorphic DNA (RAPD).  Delpinoa. 1995 - 6;  37 - 8 35-47
    PubMedGoogle Scholar
  • 10 Shirota O, Watanabe A, Yamazaki M, Saito K, Shibano K, Sekita S, Satake M. Random amplified polymorphic DNA and restriction fragemnt length polymorphism analyses of Cannabis sativa .  Natural Medicines. 1988;  52 160-6
    PubMedGoogle Scholar
  • 11 Gigliano G S. Restriction profiles of trnL (UAA) intron as a tool in Cannabis sativa L. identification.  Delpinoa. 1995 - 6;  37 - 8 85-95
    PubMedGoogle Scholar
  • 12 Gigliano G S, Caputo P. Ribosomal DNA analysis as a tool for the identification of Cannabis sativa L. specimens of forensic interest.  Science & Justice. 1997;  37 171-4
    PubMedGoogle Scholar
  • 13 Gigliano G S, Finizio A D. The Cannabis sativa L. fingerprint as a tool in forensic investigations. In: Narcotics, vols. XLIX and L, Nos. 1 and 2. 1997/1998: 129-37
    Google Scholar
  • 14 Gigliano G S. Identification of Cannabis sativa L. (Cannabaceae) using restriction profiles of the internal transcribed spacer II (IRS2).  Science & Justice. 1998;  38 225-30
    PubMedGoogle Scholar
  • 15 Gigliano G S. Preliminary data on the usefulness of internal transcribed spacer I (ITS1) sequence in Cannabis sativa L. identification.  Journal of Forensic Sciences. 1999;  44 475-7
    PubMedGoogle Scholar
  • 16 Miyahara M, Sugaya K, Tanimura A, Satake M. Nucleotide sequences of 5S-rRNA gene spacer region from Moraceae and Cannabaceae.  Natural Medicines. 1998;  52 209-12
    PubMedGoogle Scholar
  • 17 Kohjyouma M, Lee I, Iida O, Kurihara K, Yamada K, Makino Y, Sekita S, Satake M. Intraspecific variation in Cannabis sativa L. based on intergenic spacer region of chloroplast DNA.  Phar. Bull. Biological & Pharmaceutical Bulletin. 2000;  23 727-30
    PubMedGoogle Scholar
  • 18 Prevost A, Wilkinson M J. A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars.  Theoretical Applied Genetics. 1999;  98 107-12
    CrossrefPubMedGoogle Scholar
  • 19 Nei M, Li W H. Mathematical model for studying genetic variation in terms of restriction endonucleases.  Proceeding of the National Academy of Sciences of the USA. 1979;  76 5269-73
    PubMedGoogle Scholar
  • 20 Sneath P HA, Sokal R R. In: Numberical taxonomy. San Francisco; Freeman 1973
    Google Scholar

Mareshige Kojoma

Plant Functions Laboratory

RIKEN (The Institute of Physical and Chemical Research)


2-1 Hirosawa

Wako-shi

Saitama 351-0198

Japan

Fax: +81-48-467-5407

eMail: kojoma@postman.riken.go.jp