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DOI: 10.1055/s-2002-19875
© Georg Thieme Verlag Stuttgart · New York
DNA Fingerprinting of Cannabis sativa Using Inter-Simple Sequence Repeat (ISSR) Amplification
Publication History
January 19, 2001
June 9, 2001
Publication Date:
31 January 2002 (online)
![](https://www.thieme-connect.de/media/plantamedica/200201/lookinside/thumbnails/10.1055-s-2002-19875-1.jpg)
Abstract
Chemical analysis of cannabinoid, and Inter-Simple Sequence Repeat (ISSR) fingerprinting of DNA were used to identify different samples of Cannabis sativa L. for forensic purposes. Three samples were classified into two types, tetrahydrocannabinol (THC) and cannabidiol (CBD) chemo-types, by high performance liquid chromatography (HPLC). The two samples of the CBD type were not distinguished by their HPLC patterns. ISSR fingerprinting identified polymorphic DNA patterns between these samples. ISSR fingerprinting clearly differentiated between cannabis samples that could not be achieved by HPLC analysis.
Key words
Cannabis sativa - hemp - Cannabaceae - DNA profiling - ISSR - cannabinoid - HPLC
References
- 1 Quimby M W, Doorenbos N J, Turner C E, Masoud A. Mississippi-grown marihuana-Cannabis sativa cultivation and observed morphological variations. Economic Botany. 1973; 27 117-27
- 2 Small E, Beckstead H D, Chan A. The evolution of cannabinoid phenotypes in Cannabis . Economic Botany. 1975; 29 219-32
- 3 Small E, Cronquist A. A practical and natural taxonomy for Cannabis . Taxon. 1976; 25 405-35
- 4 Fetterman P S, Keith E S, Waller C W, Guerrero O, Doorenbos N J, Qinby M W. Mississippi-grown Cannabis sativa L.: preliminary observation on chemical definition of phenotype and variations in tetrahydrocannabinol content versus age, sex, and plant part. Journal of Pharmaceutical Sciences. 1971; 60 1246-9
- 5 Rowan M G, Fairbairn J W. Cannabinoid patterns in seedling of Cannabis sativa L. and their use in the determination of chemical race. Journal of Pharmaceutical Pharmacology. 1977; 29 491-4
- 6 Baker P B, Gough T A, Taylor B J. The physical and chemical features of Cannabis plants grown in the United Kingdom of Great Britain and Northern Ireland from seeds of known origin. Bulletin on Narcotics. 1982; 34 27-36
- 7 Gillian R, Cole M D, Linacre A, Thorpe J W, Watson N D. Comparison of Cannabis sativa by random amplification of polymorphic DNA (RAPD) and HPLC of cannabinoid: a preliminary study. Science & Justice. 1995; 35 169-77
- 8 Jagadish V, Robertson J, Gibbs A. RAPD analysis distinguished Cannabis sativa samples from different sources. Forensic Science International. 1996; 79 113-21
- 9 Gigliano G S, Finizio A D, Caputo P, Cozzolino S. Cannabis fingerprints by using Random Amplified Polymorphic DNA (RAPD). Delpinoa. 1995 - 6; 37 - 8 35-47
- 10 Shirota O, Watanabe A, Yamazaki M, Saito K, Shibano K, Sekita S, Satake M. Random amplified polymorphic DNA and restriction fragemnt length polymorphism analyses of Cannabis sativa . Natural Medicines. 1988; 52 160-6
- 11 Gigliano G S. Restriction profiles of trnL (UAA) intron as a tool in Cannabis sativa L. identification. Delpinoa. 1995 - 6; 37 - 8 85-95
- 12 Gigliano G S, Caputo P. Ribosomal DNA analysis as a tool for the identification of Cannabis sativa L. specimens of forensic interest. Science & Justice. 1997; 37 171-4
-
13 Gigliano G S, Finizio A D. The Cannabis sativa L. fingerprint as a tool in forensic investigations.
In: Narcotics, vols. XLIX and L, Nos. 1 and 2 . 1997/1998: 129-37 - 14 Gigliano G S. Identification of Cannabis sativa L. (Cannabaceae) using restriction profiles of the internal transcribed spacer II (IRS2). Science & Justice. 1998; 38 225-30
- 15 Gigliano G S. Preliminary data on the usefulness of internal transcribed spacer I (ITS1) sequence in Cannabis sativa L. identification. Journal of Forensic Sciences. 1999; 44 475-7
- 16 Miyahara M, Sugaya K, Tanimura A, Satake M. Nucleotide sequences of 5S-rRNA gene spacer region from Moraceae and Cannabaceae. Natural Medicines. 1998; 52 209-12
- 17 Kohjyouma M, Lee I, Iida O, Kurihara K, Yamada K, Makino Y, Sekita S, Satake M. Intraspecific variation in Cannabis sativa L. based on intergenic spacer region of chloroplast DNA. Phar. Bull. Biological & Pharmaceutical Bulletin. 2000; 23 727-30
- 18 Prevost A, Wilkinson M J. A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars. Theoretical Applied Genetics. 1999; 98 107-12
- 19 Nei M, Li W H. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proceeding of the National Academy of Sciences of the USA. 1979; 76 5269-73
- 20 Sneath P HA, Sokal R R. In: Numberical taxonomy. San Francisco; Freeman 1973
Mareshige Kojoma
Plant Functions Laboratory
RIKEN (The Institute of Physical and Chemical Research)
2-1 Hirosawa
Wako-shi
Saitama 351-0198
Japan
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Email: kojoma@postman.riken.go.jp