Application of Sequence Characterized Amplified Region (SCAR) Analysis to Authenticate Panax Species and Their Adulterants

Abstract

A 420-bp RAPD fragment from Panax quinquefolius was converted to a sequence characterized amplified region (SCAR) marker. The main difference between the SCAR of P. quinquefolius and its homolog in P. ginseng is the presence of a 25 bp insertion in the latter. Primers derived from this sequence were successfully used to authenticate six Panax species and two common adulterants.

Abbreviations

AP-PCR:arbitrarily-primed polymerase chain reaction

PCR-RFLP:polymerase chain reaction-restriction fragmentlength polymorphism

RAPD:random amplified polymorphic DNA

RAPDAG:the polymorphic band isolated from P. quinquefolius

SCAR:sequence characterized amplified region

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