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Horm Metab Res 2001; 33(9): 548-553
DOI: 10.1055/s-2001-17208
Original Clinical

© Georg Thieme Verlag Stuttgart · New York

Enhancement of β-Cell Sensitivity to Glucose by Oral Fat Load

O. Wuesten 1 , C. H. Balz 1 , H.-U. Kloer 1 , R. G. Bretzel 2 , T. Linn 2
  • 1 Division of Gastroenterology of Medical Clinic III and Policlinic of Justus Liebig University, Gießen, Germany
  • 2 Clinical Research Unit of Medical Clinic III and Policlinic of Justus Liebig University, Gießen, Germany
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Publication History

Publication Date:
17 September 2001 (online)

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Recent studies have demonstrated that 6 h infusions of lipid emulsion enhance insulin release, whereas 24 h infusions inhibit insulin secretion. How insulin release is modulated after oral fat loading has not yet been elucidated. 17 healthy fasting volunteers were subjected to 3 experiments in random order: test 1 was a frequently sampled i. v. glucose tolerance test (FSIVGTT, 0.3 g/kg glucose), test 2 began with the ingestion of 50 % sunflower oil (1.5 g/kg) followed by FSIVGTT 4 h later. Test 3 was identical to test 2 with i. v. addition of 100 U/kg heparin prior to FSIVGTT. Glucose and insulin data were analyzed by minimal model assumptions - glucose sensitivity of the β-cells (Θ1), acute insulin response (AIR) (10 min), 3 h insulin release (Θ2), glucose threshold of insulin secretion (h), insulin degradation rate (n), peripheral insulin sensitivity (SI), and glucose-dependent glucose disposal (SG). After drinking the fat emulsion, FFAs increased to 0.8 ± 0.3 mmol/l (test 2) and to 3.0 ± 0.3 mmol/l (test 3). Moderately increased FFA concentrations were associated with elevation of Θ1 (test 1, control 335 ± 157 vs. test 2: 859 ± 612 pM × min × mM-1, p = 0.030). At high plasma FFA levels and in the presence of heparin (test 3), Θ1 was reduced compared to test 2 and unchanged compared to test 1. Θ2 and h were elevated in both tests 2 and 3 compared to test 1. No changes of n, SI and SG were found. In conclusion, the ingestion of sunflower oil triglyceride emulsion resulted in a 60 % increase in plasma free fatty acids and enhanced the capacity of β-cells to secrete insulin. Heparin-induced high levels of FFA further augmented the total insulin release and inhibited parameters of glucose responsiveness.

Key words:

β-cell - Oral Fat - Free Fatty Acids

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T. Linn,M.D. 

Clinical Research Unit
Medical Clinic III and Policlinic
University of Gießen

Rodthohl 6
35392 Gießen, Germany


Phone: + 49 (641) 99 42841

Fax: + 49 (641) 99 42879

Email: thomas.linn@innere.med.uni-giessen.de

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