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Planta Med 2001; 67(6): 567-569
DOI: 10.1055/s-2001-16492
Letter

© Georg Thieme Verlag Stuttgart · New York

Vasorelaxant Effects of the Bioflavonoid Chrysin in Isolated Rat Aorta

Juan Duarte1,*, Rosario Jiménez1 , Inmaculada Concepción Villar1 , Francisco Pérez-Vizcaíno2 , José Jiménez1 , Juan Tamargo2
  • 1 Department of Pharmacology, School of Pharmacy, University of Granada, Granada, Spain
  • 2 Department of Pharmacology, School of Medicine, University Complutense of Madrid, Madrid, Spain
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Publikationsverlauf

August 4, 2000

January 28, 2001

Publikationsdatum:
17. August 2001 (online)

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Abstract

Chrysin relaxed the contractions induced by noradrenaline in isolated endothelium-intact rat aortic rings (IC50 = 16 ± 4 μM). Endothelium removal and N G-nitro-L-arginine methyl ester inhibited this relaxant effect. Chrysin potentiated the relaxation to acetylcholine under control conditions or after incubation with the superoxide anion generator hypoxanthine/xanthine oxidase. It also potentiated the relaxation induced by 3-morpholino-sydnonimine, sodium nitroprusside, and 8-bromoguanosine-3′:5′-cyclic-monophosphate. Therefore, vasorelaxation induced by chrysin in the rat aorta is endothelium- and NO-dependent. This effect is mediated by the prevention of O2 --induced inactivation of endothelial derived NO and also by the potentiation of cGMP-induced vasodilatation.

Abbreviations

L-NAME:N G-nitro-L-arginine methyl ester

NO:nitric oxide

SIN-1:3-morpholino-sydnonimine

References

  • 1 Kühnau J. The flavonoids. A class of semi-essential food components: their role in human nutrition.  World Review of Nutrition and Dietetics. 1976;  24 117-91
    PubMedSuche in Google Scholar
  • 2 Siess M H, Le Bon A M, Canivenc-Lavier M C, Amiot M J, Sabatier S, Aubert S Y,. et al . Flavonoids of honey and propolis: Characterization and effects on hepatic drug-metabolizing enzymes and benzo[a]pyrene-DNA binding in rats.  Journal of Agricultural and Food Chemistry. 1996;  44 2297-301
    CrossrefPubMedSuche in Google Scholar
  • 3 Hertog M GL, Hollman P CH, Katan M B. Content of potentially anticarcinogenic flavonoids of 28 vegetables and 9 fruits commonly consumed in the Netherlands.  Journal of Agricultural and Food Chemistry. 1992;  40 2379-83
    CrossrefPubMedSuche in Google Scholar
  • 4 Chan E CH, Pannangpetch P, Woodman O L. Relaxation to flavones and flavonols in rat isolated thoracic aorta: mechanism of action and structure-activity relationships.  Journal of Cardiovascular Pharmacology. 2000;  35 326-33
    CrossrefPubMedSuche in Google Scholar
  • 5 Duarte J, Pérez-Vizcaino F, Utrilla M P, Jiménez J, Tamargo J, Zarzuelo A. Vasodilating effects of flavonoids in rat aortic smooth muscle: structure-activity relationships.  General Pharmacology. 1993;  24 857-62
    CrossrefPubMedSuche in Google Scholar
  • 6 Herrera M D, Zarzuelo A, Jimenez J, Marhuenda E, Duarte J. Effects of flavonoids on rat aortic smooth muscle contractility: structure-activity relationships.  General Pharmacology. 1996;  27 273-7
    CrossrefPubMedSuche in Google Scholar
  • 7 Jiménez R, Andriambeloson E, Duarte J, Andriantsitohaina R, Jiménez J, Pérez-Vizcaino F ,. et al . Involvement of thromboxane A2 in the endothelium-dependent contractions induced by myricetin in rat isolated aorta.  British Journal of Pharmacology. 1999;  127 1539-44
    CrossrefPubMedSuche in Google Scholar
  • 8 Chen C K, Pace-Asciak C R. Vasorelaxing activity of resveratrol and quercetin in isolated rat aorta.  General Pharmacology. 1996;  24 857-62
    PubMedSuche in Google Scholar
  • 9 Fitzpatrick D F, Hirschfield S L, Coffey R G. Endothelium-dependent vasorelaxing activity of wine and other grape products.  American Journal of Physiology. 1993;  265 H774-78
    PubMedSuche in Google Scholar
  • 10 Furchgott R F, Vanhoutte P M. Endothelium-derived relaxing and contracting factors.  FASEB Journal. 1989;  3 2007-18
    PubMedSuche in Google Scholar
  • 11 Gryglewski R J, Palmer R M, Moncada S. Superoxide anion is involved in breakdown of endothelium-derived vascular relaxing factor.  Nature. 1986;  320 454-6
    CrossrefPubMedSuche in Google Scholar
  • 12 Mian K B, Martin W. Differential sensitivity of basal and acetylcholine-stimulated activity of nitric oxide to destruction by superoxide anion in rat aorta.  British Journal of Pharmacology. 1995;  115 993-1000
    PubMedSuche in Google Scholar
  • 13 Hecker M, Preiß C, Schini-Kerth V, Busse R. Antioxidants differentially affect nuclear factor κB-mediated nitric oxide synthase expression in vascular smooth muscle cells.  FEBS Letters. 1996;  380 224-8
    CrossrefPubMedSuche in Google Scholar
  • 14 Lipton S A, Choi Y -H, Pan Z -H, Lei S Z, Chen H -S, Sucher N J,. et al . A redox-based mechanism for the neuroprotective and neurodestructive effects of nitric oxide and related nitroso-compounds.  Nature. 1993;  364 626-32
    CrossrefPubMedSuche in Google Scholar
  • 15 Warner T D, Mitchell J A, Sheng H, Murad F. Effects of cyclic GMP on smooth muscle relaxation.  Advances in Pharmacology. 1994;  26 171-94
    PubMedSuche in Google Scholar
  • 16 Seamon K B, Daly J W. Forskolin: Its biological and chemical properties.  Advances in Cyclic Nucleotide Research. 1986;  20 1-150
    PubMedSuche in Google Scholar
  • 17 Beretz A, Anton R, Stoclet J C. Flavonoid compounds are potent inhibitors of cyclic AMP phosphodiesterase.  Experientia. 1978;  34 1054-5
    PubMedSuche in Google Scholar

Dr. Juan Duarte

Department of Pharmacology

School of Pharmacy

University of Granada

18 071 Granada

Spain

eMail: jmduarte@ugr.es

Fax: +34-958-248964