Planta Med 2001; 67(6): 587-589
DOI: 10.1055/s-2001-16483
Letter

© Georg Thieme Verlag Stuttgart · New York

Direct Amplification of Length Polymorphism Analysis Differentiates Panax ginseng from P. quinquefolius

Wai-Yan Ha1 , Forrest Chung-Fai Yau3 , Paul Pui-Hay But2,3 , Jun Wang1,3 , Pang-Chui Shaw1,3,*
  • 1 Department of Biochemistry, The Chinese University of Hong Kong, Shatin, Hong Kong, China
  • 2 Department of Biology, The Chinese University of Hong Kong, Shatin, Hong Kong, China
  • 3 Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, China
Further Information

Publication History

September 1, 2000

December 2, 2000

Publication Date:
17 August 2001 (online)

Abstract

The method of direct amplification of length polymorphism (DALP) was applied to authenticate Panax ginseng and P. quinquefolius. A 636-bp DALP fragment was present in all P. ginseng but absent in all the P. quinquefolius cultivars examined. We have shown that the use of DALP and conversion of specific polymorphic band to sequence-tagged site (STS) for quick authentication may be applied to authenticate related medicinal materials.

Abbreviations

DALP:direct amplified of length polymorphism

STS:sequence-tagged site

References

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  • 2 Cao H, But P PH, Shaw P C. A molecular approach to identification of the Chinese Drug ‘Pu Gong Ying’ (Herba Taraxaci) and six adulterants by DNA fingerprinting using random primed polymerase chain reaction (PCR).  Journal of Chinese Pharmaceutical Sciences. 1996;  5 186-94
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  • 8 Wang J, Ha W Y, Ngan F N, But P PH, Shaw P C. Application of sequence characterized amplified region (SCAR) analysis to authenticate Panax species and their adulterants. Planta Medica in press
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Dr. Pang-Chui Shaw

Department of Biochemistry

The Chinese University of Hong Kong

Shatin

Hong Kong

China

Email: pcshaw@cuhk.edu.hk

Fax: 852-26035123