Download PDF
Horm Metab Res 2001; 33(1): 57-61
DOI: 10.1055/s-2001-12628
Original Clinical
© Georg Thieme Verlag Stuttgart · New York

Nutrient Regulation of Post-Heparin Lipoprotein Lipase Activity in Obese Subjects

L. R. Ranganath1 ,   3 , J. M. Beety2 , J. Wright2 , L. M. Morgan2
  • 1 Department of Chemical Pathology, Epsom General Hospital, Epsom, Surrey, UK
  • 2 School of Biological Sciences, University of Surrey, Guildford, Surrey, UK
Further Information

Publication History

Publication Date:
31 December 2001 (online)

Also available at
    Permissions and Reprints

This study examines the immediate effect of ingestion of oral carbohydrate and fat on lipoprotein lipase (LPL) activity post-heparin in six lean and six obese age-matched women. Subjects were given, on two separate occasions, 340 kcal carbohydrate or an equicaloric amount of fat, both in 300 ml of water. Post-heparin LPL activity (10 000 U) was measured on each occasion 120 minutes after ingestion of the meal. Following oral carbohydrate postprandial plasma insulin levels were significantly higher in obese subjects than in lean (p < 0.01). Impaired glucose tolerance was seen in the obese group. GIP secretion was similar in lean and obese subjects both during oral fat and carbohydrate ingestion. GLP-1 secretion post-carbohydrate was lower in obese subjects. Total LPL activity unadjusted for body weight was similar in the two groups after carbohydrate administration but was significantly lower when adjusted per kg body weight. Total LPL activity was lower in the lean group at 130 minutes after fat administration (p < 0.02). Fasting serum triglycerides were higher in the obese group and were inversely related to the post-carbohydrate LPL activity (r = - 0.65, p < 0.02). lntraluminal lipoprotein lipase activity is not increased in established obesity. Fat and carbohydrate nutrients may affect LPL activity differently in lean and obese subjects.

Key words:

Obesity - Insulin - GIP - GLP-1 - Carbohydrate - Fat - Heparin - Plasma Lipoprotein Lipase Activity

References

  • 1 Eckel R H. Lipoprotein lipase. A multifunctional enzyme relevant to common metabolic diseases.  NEJM. 1989;  320 1060-1068
    PubMedGoogle Scholar
  • 2 Yost T J, Eckel R H. Fat calories may be preferentially stored in reduced-obese women: a permissive pathway for the resumption of the obese state.  J Clin Endocrinol Metab. 1988;  67 259-264
    PubMedGoogle Scholar
  • 3 Fried S K, Turkenkopf I J, Goldberg I J, et al. Mechanism of increased lipoprotein lipase in fat cells of obese Zucker rats.  Am J Physiol. 1991;  261 E653-E660
    PubMedGoogle Scholar
  • 4 Bessessen D H, Robertson A D, Eckel R H. Weight reduction increases adipose but decreases cardiac lipoprotein lipase in reduced-obese Zucker rats.  Am J Physiol. 1991;  261 E246-E251
    PubMedGoogle Scholar
  • 5 Pykalisto O J, Smith P H, Brunzell J D. Determinants of human adipose tissue lipoprotein lipase: effects of diabetes and obesity on basal and diet-induced activity.  J Clin Invest. 1975;  56 1108-1117
    PubMedGoogle Scholar
  • 6 Taskinen M R, Nikkila E A. Lipoprotein lipase activity in adipose tissue and postheparin plasma in human obesity.  Acta Medica Scandinavica. 1977;  202 399-408
    PubMedGoogle Scholar
  • 7 Sjostrom L. Methods for measurement of total and visceral adipose tissue volume and relationships between visceral fat and disease in 1006 severely obese subjects. In: Oomura Y, et al. (eds). Progress in Obesity Research 1990. John Libbey & Co. Ltd. 1990: 323-334
    Google Scholar
  • 8 Inoue S. Metabolic effects of autonomic nervous activity in development of obesity. In: Oomura Y, et al. (eds). Progress in Obesity Research. John Libby & Company Ltd 95-101
    Google Scholar
  • 9 Semenkovitch C F, Wims M, Noe L, Etienne J, Chan L. Insulin regulation of lipoprotein lipase activity in 3T3-L1 adipocytes is mediated at posttranscriptional and posttranslational levels.  J Biol Chem. 1989;  264 9030-9038
    PubMedGoogle Scholar
  • 10 Glass A R, Burmann K D, Dahms W T, Boehm T M. Endocrine function in human obesity.  Metabolism. 1981;  30 89-104
    CrossrefPubMedGoogle Scholar
  • 11 Eckel R H, Fujimoto W J, Brunzell J D. Gastric inhibitory polypeptide enhances lipoprotein lipase activity in cultured pre-adipocytes.  Diabetes. 1978;  28 1141-1142
    PubMedGoogle Scholar
  • 12 Oben J, Morgan L, Fletcher J, Marks V. Effects of entero-pancreatic hormones, gastric inhibitory polypeptide and glucagon-like peptide-1 (7 - 36) amide on fatty acid synthesis in explants of rat adipose tissue.  J Endocrinol. 1989;  130 267-272
    PubMedGoogle Scholar
  • 13 Knapper J M, Puddicombe S M, Morgan L M, Fletcher J M. Investigations into the actions of glucose-dependent insulinotropic polypeptide and glucagon-like polypeptide-1 (7-36)amide on lipoprotein lipase activity in explants of rat adipose tissue.  J Nutr. 1995;  125 183-188
    PubMedGoogle Scholar
  • 14 Karpe F, Olivecrona T, Walldius G, Hamsten A. Lipoprotein lipase in plasma after an oral fat load: relation to free fatty acids.  J Lipid Res. 1992;  33 975-984
    PubMedGoogle Scholar
  • 15 Hampton S M, Whitey L. Monitoring B-cell responses in obese and normal weight subjects: a pilot study.  DiabÉte et Metabolisme. 1993;  19 582-585
    PubMedGoogle Scholar
  • 16 Morgan L M, Morris B A, Marks V. Radioimmunoassay of gastric inhibitory polypeptide.  Ann Clin Biochem. 1978;  15 172-177
    PubMedGoogle Scholar
  • 17 Elliott R M, Morgan L M, Tredger J A, Deacon S, Wright J, Marks V. Glucagon-like peptide-1, (7-36) amide and glucose dependent insulinotropic polypeptide in response to nutrient ingestion in man. Acute postprandial and 24 hr secretion pattern.  J Endocrinol. 1993;  138 159-166
    PubMedGoogle Scholar
  • 18 Ranganath L, Beety J M, Morgan L M, Wright J W, Howland R, Marks V. Attenuated GLP-1 secretion in obesity: cause or consequence.  Gut. 1996;  38 916-919
    PubMedGoogle Scholar
  • 19 Camps L, Reina M, Llobera M, Bengtsson-Olivecrona G, Olivecrona T, Vilaro S. Lipoprotein lipase in lungs, spleen, and liver: synthesis and distribution.  J Lipid Res. 1991;  32 1877-1888
    PubMedGoogle Scholar
  • 20 Camps L, Reina M, Llobera M, Vilaro S, Olivecrona T. Lipoprotein lipase: cellular origin and functional distribution.  Am J Physiol. 1990;  258 C673-C681
    PubMedGoogle Scholar
  • 21 Forbes G B. Human body composition.  New York:; The Adult Publishers Inc. 1987
    Google Scholar
  • 22 Saxena U, Witte L D, Goldberg U. Release of endothelial cell lipoprotein lipase by plasma lipoproteins and free fatty acids.  J Biol Chem. 1989;  264 4349-4355
    PubMedGoogle Scholar
  • 23 Saxena U, Klein M G, Goldberg I J. Transport of lipoprotein lipase across endothelial cells.  Proc Natl Acad Sci USA. 1991;  88 2254-2258
    PubMedGoogle Scholar
  • 24 Saxena U, Klein M G, Goldberg I J. Metabolism of endothelial cell-bound lipoprotein lipase. Evidence for heparan sulphate proteoglycan-mediated internalisation and recycling.  J Biol Chem. 1990;  265 12 880-12 886
    PubMedGoogle Scholar
  • 25 Couillard C, Bergeron N, Prud’homme D, Bergeron J, Tremblay A, Bouchard C, Mauriege P, Despres J P. Postprandial triglyceride response in visceral obesity in men.  Diabetes. 1998;  47 953-960
    PubMedGoogle Scholar
  • 26 Ranganath L R, Beety J M, Morgan L M. Inhibition of insulin. glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1) secretion by octreotide has no effect on post-heparin plasma lipoprotein lipase activity.  Horm Metab Res. 1999;  31 262-266
    PubMedGoogle Scholar

Dr. L. Ranganath

Department of Clinical Chemistry
Royal Liverpool University Hospital

Prescot Street
Liverpool, L7 8XP
UK


Phone: Phone:+ 44 (51) 706 2000 Ext. 4230

Fax: Fax:+ 44 (51) 706 5813

Email: E-mail:lrang@liv.ac.uk