Inhibitory Activity of Stilbenes from Medicinal Plants on the Expression of Cell Adhesion Molecules on THP1 Cells

Abstract

The inhibitory activity of stilbenes isolated from medicinal plants on cell adhesion molecules on the surface of THP-1 human monocytic cell lines was investigated. Among ten stilbenes tested, four stilbenes displayed a significant inhibitory activity on the expression of both intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1). A cell-to-cell adhesion assay showed that 3,5-dihydroxy-4′-methoxystilbene and 2,3,4′,5-tetrahydroxystilbene-2-O-β-D-glucopyranoside as well as resveratrol blocked significantly TNF-α-inducing cell-cell adhesion between human umbilical vein endothelial cells (HUVEC) and THP-1 cells.

Abbreviations

ELISA:Enzyme linked immunoadsorbent assayFBS:Fetal bovine serumHUVEC:Human umbilical vein endothelial cellICAM-1:Intercellular adhesion molecule-1OPD:O-Phenylenediamine dihydrochloridePBS:Phosphate buffered salineTNF-α:Tumor necrosis factor-αVCAM-1:Vascular cell adhesion molecule-1

References

• 1 Dustin  M L,, Rothlein  R,, Bhan  A K,, Dinarello  C A,, Springer  T A.. Induction by IL-1 and interferon-gamma: tissue distribution, biochemistry, and function of a natural adherence molecule (ICAM-1).  Journal of Immunology. 1986;;  137 (1) 245-54
• 2 Wegner  C D,, Gundel  R H,, Reilly  P,, Haynes  N,, Letts  L G,, Rothlein  R.. Intercellular adhesion molecule-1 (ICAM-1) in the pathogenesis of asthma.  Science. 1990;;  247 (4941) 456-9
• 3 Buckle  A M,, Hogg  N.. Human memory T cells express intercellular adhesion molecule-1 which can be increased by interleukin 2 and interferon-gamma.  European Journal of Immunology. 1990;;  20 337-41
• 4 Webb  D S,, Mostowski  H S,, Gerrard  T L.. Cytokine-induced enhancement of ICAM-1 expression results in increased vulnerability of tumor cells to monocyte-mediated lysis.  Journal of Immunology. 1991;;  146 3682-6
• 5 Rothlein  R,, Czajkowski  M,, O'Neil  M M,, Marlin  S D,, Mainolfi  E,, Merluzzi  V J.. Induction of intercellular adhesion molecule 1 on primary and continuous cell lines by pro-inflammatory cytokines. Regulation by pharmacologic agents and neutralizing antibodies.  Journal of Immunology. 1988;;  141 1665-9
• 6 Cronstein  B N,, Kimmel  S C,, Levin  R I,, Martiniuk  F,, Weissmann  G.. A mechanism for the anti-inflammatory effects of corticosteroids: the glucocorticoid receptor regulates leukocyte adhesion to endothelial cells and expression of endothelial-leukocyte adhesion molecule 1 and intercellular adhesion molecule 1.  Proceedings of National Academy of Sciences. 1992;;  89 9991-5
• 7 Ferrero  M E,, Bertelli  A A,, Fulgenzi  A,, Pellegatta  P,, Corsi  M M,, Bonfrate  M,, Ferrara  F,, De Caterina  R,, Giovannini  L,, Bertelli  A.. Activity in vitro of resveratrol on granulocyte and monocyte adhesion to endothelium.  American Journal of Clinical Nutrition. 1998;;  68 1208-14
• 8 Oh  S R,, Ryu  S Y,, Park  S H,, Jung  K Y,, Lee  I S,, Ahn  K S,, Lee  J J,, Lee  H K.. Anticomplementary activity of stilbenes from medicinal plants.  Archives of Pharmacal Research. 1998;;  21 (6) 703-6
• 9 Vennegoor  C J,, Van de Wiel-van Kemenade  E,, Huijbens  R J,, Sanchez-Madrid  F,, Melief  C J,, Figdor  C G.. Role of LFA-1 and VLA-4 in the adhesion of cloned normal and LFA-1 (CD11/CD18)-deficient T cells to cultured endothelial cells. Indication for a new adhesion pathway.  Journal of Immunology. 1992;;  148 1093-101
• 10 Gybulsky  M I,, Gimbrone  M A.. Endothelial expression of a mononuclear leukocyte adhesion molecule during atherogenesis.  Science. 1991;;  251 788-91
• 11 Kimura  Y,, Okuda  H,, Arichi  S.. Effects of stilbenes on arachidonate metabolism in leukocytes.  Biochimica et Biophysica Acta. 1985;;  834 (2) 275-8
• 12 Subbaramaiah  K,, Chung  W J,, Michaluart  P,, Telang  T,, Tanabe  T,, Inoue  H,, Jang  M,, Pezzuto  J M,, Dannenberg  A J.. Resveratrol inhibits cyclooxygenase-2 transcription and activity in phorbol ester-treated human mammary epithelial cells.  Journal of Biological Chemistry. 1998;;  273 (34) 21875-82
• 13 Wadsworth  T L,, Koop  D R.. Effects of the wine polyphenolics quercetin and resveratrol on pro-inflammatory cytokine expression in RAW 264.7 macrophages.  Biochemical Pharmacology. 1999;;  57 (8) 941-9
• 14 Tsai  S H,, Lin-Shiau  S Y,, Lin  J K.. Suppression of nitric oxide synthase and the down-regulation of the activation of NFkappaB in macrophages by resveratrol.  British Journal of Pharmacology. 1999;;  126 (3) 673-80
• 15 Ferrero  M E,, Bertelli  A E,, Fulgenzi  A,, Pellegatta  F,, Corsi  M M,, Bonfrate  M,, Ferrara  F,, De Caterina  R,, Giovannini  L,, Bertelli  A.. Activity in vitro of resveratrol on granulocyte and monocyte adhesion to endothelium.  American Journal of Clinical Nutrition. 1998;;  68 (6) 1208-14
• 16 Roebuck  K A,, Finnegan  A.. Regulation of intercellular adhesion molecule-1 (CD54) gene expression.  Journal of Leukocyte Biology. 1999;;  66 (6) 876-88
• 17 Quinlan  K L,, Naik  S M,, Cannon  G,, Armstrong  C A,, Bunnett  N W,, Ansel  J C,, Caughman  S W.. Substance P activates coincident NF-AT- and NF-kappa B-dependent adhesion molecule gene expression in microvascular endothelial cells through intracellular calcium mobilization.  Journal of Immunology. 1999;;  163 (10) 5656-65
• 18 Skehan  P,, Storeng  R,, Scudiero  D,, Monks  A,, McMahon  J,, Vistica  D,, Warren  J T,, Bokesch  H,, Kenney  S,, Boyd  M R.. New colorimetric cytotoxicity assay for anticancer-drug screening.  Journal of National Cancer Institute. 1990;;  82 1107-12