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Gesundheitswesen 2000; 62(11): 604-608
DOI: 10.1055/s-2000-13042
© Georg Thieme Verlag Stuttgart · New York

Verfolgung von Gruppenerkrankungen mit Norwalk-like-Viren (NLV) in Baden-Württemberg

Isolierung und Detektion von Norwalk-like-Virus-RNA aus Stuhlproben mittels RT-PCRSurveillance of Norwalk associated group infections in Baden-Württemberg. Isolation and detection of Norwalk-RNA from stool samples by RT-PCRJ. Fleischer, C. Wagner Wiening, P. Kimmig
  • Landesgesundheitsamt Baden-Württemberg
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Publication History

Publication Date:
31 December 2000 (online)

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Zusammenfassung

In den Jahreswechseln 98/99 und 99/2000 wurden von mehreren Gesundheitsämtern in Baden-Württemberg im Rahmen von Gruppenerkrankungen zahlreiche Stuhlproben zur Untersuchung auf gastrointestinale Erreger an das Landesgesundheitsamt Baden-Württemberg eingesandt. Während die bakteriologischen Routineuntersuchungen sowie Virus-Antigen-Untersuchungen auf Adeno-, Astro- und Rotaviren negativ blieben, ergaben zusätzliche RT-PCR-Untersuchungen auf Norwalkviren vermehrt positive Befunde. Insgesamt wurden im LGA im Zeitraum von Dezember 98 bis Januar 2000 aus 14 Gruppenerkrankungen 286 Stuhlproben mittels RT-PCR untersucht, wobei in 118 Fällen Norwalk-Virus-RNA nachgewiesen werden konnte. Zur Verifizierung der Analysen wurden Stichproben von einzelnen Gruppenerkrankungen an das Robert-Koch-Institut, Abt. Molekulare Virologie, verschickt; hier wurde der Befund „Norwalk-Viren” bestätigt.

Surveillance of Norwalk associated group infections in Baden-Württemberg. Isolation and detection of Norwalk-RNA from stool samples by RT-PCR

Between the turn of the years 98/99 and 99/2000, stool samples from various health departments in the south of Germany were sent to the LGA (Landesgesundheitsamt Baden-Württemberg). Different testings as bacteriological routines for enterobacteriaceae and virological antigen screenings of the stool samples for adeno-, astro- and rotaviruses lead to negative results. Additional RT-PCR tests for the presence of Norwalk-RNA showed nearly 40 % of positive samples. From 14 group infections between december 98 and january 2000, 286 samples in total were sent to the LGA, 118 of them gave positive results for the norwalk agent. For the verification of the analysis, some random samples were sent to the Robert-Koch-Institut, div. of molecular virology at Berlin, where the results were confirmed.

Keywords

Norwalk-Like-Virus(NLV) - Virus-RNA - Norwalk-Associated Groupinfections - Stool Samples - RT-PCR

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Dr. J. Fleischer

Landesgesundheitsamt Baden-Württemberg

Wiederholdstraße 15

70174 Stuttgart