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Synlett 1999; 1999(11): 1778-1780
DOI: 10.1055/s-1999-2945
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Efficient and Rapid Coupling of Nucleosides to Amino Derivatized Solid-Phase Supports

Richard T. Pon* , Shuyuan Yu
  • *Dept. of Molecular Biology and Biochemistry, 3350 Hospital Drive N.W., University of Calgary, Calgary, AB, Canada T2N 4N1; Fax (4 03) 2 83-49 07; E-mail: rtpon@ucalgary.ca
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Publication Date:
31 December 1999 (online)

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High loading efficiency (up to 96%) and rapid coupling (5 min) of nucleosides to amino solid-phase supports can be obtained using HBTU and DMAP as coupling reagents in acetonitrile. Pyridine or halogenated solvents can be eliminated and the method is amenable to both automation and large-scales.

nucleoside coupling - oligonucleotide synthesis - support derivatization - DMAP - HBTU

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