Download PDF
Synlett 1999; 1999(10): 1667-1678
DOI: 10.1055/s-1999-2914
new tools in synthesis
© Georg Thieme Verlag, Rüdigerstr. 14, 70469 Stuttgart, Germany. All rights reserved. This journal, including all individual contributions and illustrations published therein, is legally protected by copyright for the duration of the copyright period. Any use, exploitation or commercialization outside the narrow limits set by copyright legislation, without the publisher's consent, is illegal and liable to criminal prosecution. This applies in particular to photostat reproduction, copying, cyclostyling, mimeographing or duplication of any kind, translating, preparation of microfilms, and electronic data processing and storage.

Fluorescent Oligonucleotides - Versatile Tools as Probes and Primers for DNA and RNA Analysis

Christian Wojczewski* , Karen Stolze, Joachim W. Engels
  • *Institut für Organische Chemie, Johann Wolfgang Goethe-Universität, Marie-Curie-Str. 11, 60439 Frankfurt am Main, Germany; Fax (+ 49)69 79 82 91 48; E-mail: engels@ews1.org.chemie.uni-frankfurt.de
Further Information

Publication History

Publication Date:
31 December 1999 (online)

  PDF Download Permissions and Reprints All articles of this category

Different strategies for the fluorescence labeling of nucleosides and oligonucleotides and techniques for their detection are discussed. Pre- and post-synthetic routes as well as automated solid phase-based syntheses and enzymatic methods are compared. Tailor-made chemical syntheses are presented that meet the requirements of a broad spectrum of applications for oligonucleotides as probes.

fluorescent oligonucleotides - automated solid-phase synthesis - modified nucleosides - fluorescence spectroscopy - DNA

      >