Planta Med 1999; 65(1): 060-063
DOI: 10.1055/s-1999-13964
Original Paper

Georg Thieme Verlag Stuttgart · New York

Influence of Agrobacterium rhizogenes Strains on Biomass and Alkaloid Productivity in Hairy Root Lines of Hyoscyamus muticus and H. albus [ *]

M. Zehra1 , S. Banerjee1 , S. Sharma2 , Sushil Kumar1
  • 1VGV;Genetics Resources and Biotechnology Division, CIMAP, Lucknow, India
  • 2Data Processing and Documentation Division, Central Institute of Medicinal and Aromatic Plants, CIMAP Lucknow, India
Further Information

Publication History

March 16, 1998

July 5, 1998

Publication Date:
31 December 1999 (online)

Abstract:

Using leaf explants of in vitro grown Hyoscyamus albus and H. muticus plantlets, hairy roots were induced following inoculation with Agrobacterium rhizogenes strains A4 and LBA-9402. The transformed roots, appearing after 14 - 17 days incubation on hormone-free MS medium containing 1 g/L cephalexin, were excised and maintained in the same medium. Ten randomly selected hairy root lines from each bacterial treatment of the two plant systems were compared for growth and alkaloid production in half-strength, hormone-free MS medium on 25th day of culture. A. rhizogenes strain - A4 induced hairy root lines of both H. albus and H. muticus were comparatively faster growing than those induced by strain LBA-9402. In contrast to earlier reports, some of the hairy root lines of H. albus induced by A. rhizogenes strain A4 were as fast growing as the hairy root lines of H. muticus. The atropine yields of A4 induced lines of H. albus were significantly higher (3.5 fold) than the LBA-9402 induced lines. No such relationship between the bacterial strain and alkaloid productivity could, however, be obtained in case of hairy root lines of H. muticus.

1 CIMAP Publication No. 97-82J

1 CIMAP Publication No. 97-82J