Download PDF
Synlett 1995; 1995(2): 205-206
DOI: 10.1055/s-1995-4897
letter
© Georg Thieme Verlag, Rüdigerstr. 14, 70469 Stuttgart, Germany. All rights reserved. This journal, including all individual contributions and illustrations published therein, is legally protected by copyright for the duration of the copyright period. Any use, exploitation or commercialization outside the narrow limits set by copyright legislation, without the publisher's consent, is illegal and liable to criminal prosecution. This applies in particular to photostat reproduction, copying, cyclostyling, mimeographing or duplication of any kind, translating, preparation of microfilms, and electronic data processing and storage.

Preparation of N-9-Fluorenylmethyloxycarbonyl-Asparagine-Pentafluorophenyl Ester from the Free Acid

Jeffrey I. Gyi* , Richard G. Kinsman, Anthony R. Rees
  • *School of Biology and Biochemistry, University of Bath, Claverton Down, Bath, Avon, BA2 7AY, U.K.
Further Information

Publication History

Publication Date:
31 December 2000 (online)

  PDF Download  Permissions and Reprints All articles of this category

We report a new method for synthesising Fmoc-asparagine pentafluorophenyl ester from the free acid which is suitable for use directly in peptide assemblies. The coupling reagent 1-(3-dimethylaminopropyl)-3-ethylcarbodi-imide hydrochloride (EDC) is found to be highly effective for activating non-sidechain protected asparagine and it facilitates an efficient water-based work-up. Yields of product are typically in the range of 65-75%.

Solid phase peptide synthesis - Fmoc-Asn-OPfp - Isotope-edited NMR spectroscopy