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CC BY 4.0 · Ibnosina Journal of Medicine and Biomedical Sciences
DOI: 10.1055/s-0045-1815704
Original Article

KI-67 Immunohistochemical Expression in Lung Tissue of Rats under Chronic Renal Failure

Authors

  • Dilora Botirovna Avezova 

    1   Department of Anatomy and Clinical Anatomy, Bukhara State Medical Institute, Bukhara, Republic of Uzbekistan

  • Tolibjon Bakhtiyor Ugli Tilavov 

    2   Department of Histology, Cytology and Embriology, Bukhara State Medical Institute, Bukhara, Uzbekistan

  • Dilnoza Akhrorovna Khasanova 

    1   Department of Anatomy and Clinical Anatomy, Bukhara State Medical Institute, Bukhara, Republic of Uzbekistan

Funding and Sponsorship This work was supported by Bukhara State Medical Institute.
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Abstract

Background

Chronic kidney disease (CKD) is associated with systemic complications, including pulmonary alterations due to kidney–lung crosstalk. This study evaluates Ki-67 immunohistochemical expression in rat lung tissue to assess cellular proliferative activity under experimental chronic renal failure (CRF) conditions, considering age-related variations.

Materials and Methods

Outbred male rats (n = 150, aged 6, 9, and 12 months) were divided into control and experimental groups. CRF was induced via repeated intramuscular injections of 50% glycerol (0.5 mL twice weekly for 1 month). Alveolar lung tissues were fixed, sectioned, and stained for Ki-67 using the 3,3′-Diaminobenzidine method. Quantitative analysis was performed with QuPath software (v0.4.0) to calculate proliferative indices. Data were analyzed using two-way ANOVA with post-hoc tests (p <0.05).

Results

In controls, Ki-67 proliferative indices declined with age: 55.86% at 6 months, 52.32% at 9 months, and 23.44% at 12 months. Under CRF, indices were significantly suppressed: 21.75, 11.06, and 2.32%, respectively (p <0.01 vs. controls), indicating impaired cellular renewal.

Conclusion

Experimental CRF exacerbates age-dependent reductions in lung cell proliferation, highlighting mechanisms, such as inflammation and hypoxia in pulmonary pathology. These findings underscore the need for targeted interventions in CKD-related lung dysfunction.

Keywords

lung tissue - regeneration - aging - cell proliferation - chronic renal failure - immunohistochemistry - Ki-67

Authors' Contributions

T.T.B.U. collected the data. A.D.B. prepared the manuscript draft. K.D.A. reviewed and edited the manuscript. All authors have read and approved the final version of the manuscript.


Artificial intelligence (AI) tools, including language models, have been used solely for linguistic purposes, such as translation and grammatical correction. No part of the scientific content, data analysis, interpretation, or conclusions was generated using AI.


Declaration of GenAI Use

Artificial intelligence (AI) tools, including language models, have been used solely for linguistic purposes, such as translation and grammatical correction. No part of the scientific content, data analysis, interpretation, or conclusions was generated using AI.


Compliance with Ethical Principles

Ethical approval was obtained from the Local Ethics Committee of Bukhara State Medical Institute (Protocol No. 5, November 27, 2024; Extract No. 12028 dated December 02, 2024).




Publication History

Article published online:
12 January 2026

© 2026. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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