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CC BY 4.0 · Indian J Plast Surg 2025; 58(06): 429-435
DOI: 10.1055/s-0045-1809151
Original Article

Impact of Hyaluronic Acid Lip Filling on the Electromyographic Activity of the Orbicularis Oris Muscle in Adult Women

Authors

  • Nicole Barbosa Bettiol

    1   Department of Basic and Oral Biology, Ribeirão Preto School of Dentistry, University of São Paulo, São Paulo, Brazil

  • Selma Siéssere

    1   Department of Basic and Oral Biology, Ribeirão Preto School of Dentistry, University of São Paulo, São Paulo, Brazil
    2   National Science and Technology Institute for Translational Medicine, São Paulo, Brazil

  • Mirella Milla Marino

    1   Department of Basic and Oral Biology, Ribeirão Preto School of Dentistry, University of São Paulo, São Paulo, Brazil

  • Laís Valencise Magri

    3   Department of Restorative Dentistry, Ribeirão Preto School of Dentistry, University of São Paulo, São Paulo, Brazil

  • Jardel Francisco Mazzi-Chaves

    3   Department of Restorative Dentistry, Ribeirão Preto School of Dentistry, University of São Paulo, São Paulo, Brazil

  • Paulo Batista de Vasconcelos

    1   Department of Basic and Oral Biology, Ribeirão Preto School of Dentistry, University of São Paulo, São Paulo, Brazil

  • Alice Helena de Lima Santos Cardoso

    1   Department of Basic and Oral Biology, Ribeirão Preto School of Dentistry, University of São Paulo, São Paulo, Brazil

  • Thamyres Branco

    1   Department of Basic and Oral Biology, Ribeirão Preto School of Dentistry, University of São Paulo, São Paulo, Brazil

  • Isabela Hallak Regalo

    1   Department of Basic and Oral Biology, Ribeirão Preto School of Dentistry, University of São Paulo, São Paulo, Brazil

  • Simone Cecilio Hallak Regalo

    1   Department of Basic and Oral Biology, Ribeirão Preto School of Dentistry, University of São Paulo, São Paulo, Brazil
    2   National Science and Technology Institute for Translational Medicine, São Paulo, Brazil

  • Marcelo Palinkas 

    1   Department of Basic and Oral Biology, Ribeirão Preto School of Dentistry, University of São Paulo, São Paulo, Brazil
    2   National Science and Technology Institute for Translational Medicine, São Paulo, Brazil

Funding This study was supported by a grant from the Fundação de Amparo à Pesquisa do Estado de São Paulo- FAPESP, CNPQ Process: 312586/2021-3 and National Institute and Technology - Translational Medicine (INCT.TM).
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Abstract

Introduction

The rising use of orofacial harmonization highlights the importance of the lip area, closely linked to the orbicularis oris muscle. This longitudinal study assessed the electromyographic (EMG) activity of the upper and lower orbicularis oris muscle in adult women following hyaluronic acid lip filling.

Materials and Methods

Twenty-two adult women, with a mean age of 35.4 ± 12.3 years, were included. EMG was measured during rest, cheek inflation tasks (concurrently and alternately), lip protrusion and compression, and before, 30, and 60 days after lip filling. Differences were significant using t-test and repeated measures analysis of variance with Bonferroni correction (p < 0.05).

Results

Significant EMG differences were found in the upper orbicularis oris muscle (before vs. 30 days, p = 0.04) and lower orbicularis oris muscle (before vs. 30 days, p = 0.0006) during bilateral cheek inflation, and in the upper orbicularis oris muscle (30 vs. 60 days, p = 0.05) during rest. At 30 days, EMG of both upper and lower orbicularis oris muscle decreased in all tasks. By 60 days, the upper muscle's EMG increased in most tasks, while the lower muscle's EMG decreased during cheek inflation tasks. The lower orbicularis oris muscle had significantly higher EMG than the upper in nearly all tasks (p = 0.000).

Conclusion

Hyaluronic acid lip filling initially relaxed the orbicularis oris muscle at 30 days. By 60 days, the upper muscle's activity increased, indicating adaptation, while the lower muscle remained less active during cheek inflation, showing distinct functional changes after 60 days.

Keywords

hyaluronic acid - lips - orbicularis oris muscle - facial harmonization - EMG


Publication History

Article published online:
19 May 2025

© 2025. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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