Optimization of the Spin Cycle for a Platelet-Rich Plasma System for Use in Dogs

Abstract

Introduction: Despite its common use, very little is known about how different spin cycle protocols affect the preparation of platelet rich plasma (PRP). The purpose of this study was to evaluate the effect of variable centrifugal force and spin times for preparation of PRP from canine blood using the Arthrex ACP Max device on platelet concentration, leukocyte reduction, and growth factor concentrations. We hypothesized that higher centrifugation spinning rates would yield less concentration of platelets and growth factors in the final product with increased leukocyte concentrations.

Materials and Methods: Healthy dogs weighing at least 27 kg, 12 months of age or older with no comorbidities were enrolled. Dogs were and whole blood (WB) was. The Max device was filled and centrifuged using four different initial spin cycles. A CBC and ELISA for PDGF and TGF-β was performed on 1 mL of final product.

Results: All spin cycles increased platelet concentration, decreased erythrocytes and leukocytes compared to WB. No significant difference was seen between groups. A significant increase in TGF-β was seen in all spin cycles versus WB, but not across groups.

Discussion/Conclusion: Phase II found spinning at lower rpm yielded the highest concentration of platelets and PDGF-BB. However, it contained larger amounts of white blood cells than other spin cycles, support our hypothesis. What is unknown is the optimal makeup of PRP. Limitations of this study include small sample size.

Acknowledgements: Intramural funding supported this project and Arthrex Inc. donated kits.

Publication History

Article published online:
16 September 2024