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Open Access
CC BY 4.0 · Eur J Dent 2025; 19(01): 116-123
DOI: 10.1055/s-0044-1787958
Original Article

Identification and Characterization of Key Genes Associated with Amelogenesis

Authors

  • Tahsinul Haque

    1   Preventive Dental Sciences Department, College of Dentistry, Dar Al Uloom University, Riyadh, Saudi Arabia

  • Fatema Akhter

    2   Surgical and Diagnostic Sciences Department, College of Dentistry, Dar Al Uloom University, Riyadh, Saudi Arabia

  • Nourelhoda Alim

    3   Surgical and Diagnostic Sciences Department, College of Dentistry, Dar Al Uloom University, Riyadh, Saudi Arabia

  • Abdullah Nabhan

    4   Surgical and Diagnostic Sciences Department, Collage of Dentistry, Prince Sattam Bin Abdulaziz University, Al Kharj, Saudi Arabia

  • Fawzia Al kahtani

    5   Dental Public Health, Private Sector, Riyadh, Saudi Arabia

  • Abdullah Mohammed Sambawa

    6   Primary Healthcare Center, Ministry of Health, Riyadh, Saudi Arabia
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Abstract

Objectives The identification of key genes associated with amelogenesis would be helpful in finding solutions to genetic disorders in oral biology. The study aimed to use in silico analysis to identify the key genes involved in tooth development associated with preameloblasts (PABs) and secretory ameloblasts (SABs).

Material and Methods The data was subjected to quality analysis and uniform manifold approximation and projection analysis. To examine the distribution of the genes and identify important upregulated loci, a p-value histogram, a quantile plot, a mean difference and mean-variance plot, and a volcano plot were generated. Finally, protein-protein interaction and gene enrichment analyses were performed to determine the ontology, relevant biological processes, and molecular functions of selected genes.

Results A total of 157 genes were found to be significant in the PAB versus SAB comparison. HIST1H31 revealed strong interaction with HIST1H2BM, and EXO1, ASPM, SPC25, and TTK showed strong interactions with one other. The STRING database revealed that NCAPG, CENPU, NUSAP1, HIST1H2BM, and HIST1H31 are involved in biological processes. NCAPG, CENPU, SPC25, ETV5, TTK, ETV1, FAM9A, NUSAP1, HIST1H2BM, and HIST1H31 are involved in cellular components.

Conclusion The TTK, NUSAP1, CENPU, NCAPG, FAM9A, ASPM, SPC25, and HIST1H31 genes demonstrate functions in cell division. These genes might play a role in ameloblast development. These results will be useful in developing new methods to stimulate ameloblast development, which is essential for tooth regeneration and tissue engineering. However, more research is required to validate the functions of these genes and the genes with which they interact. A wide variety of genetic, epigenetic, and exogenous signaling factors regulate these genes and pathways throughout development and differentiation, cell fate, and behavior.

Keywords

amelogenesis - genes - genetics - preameloblasts - secretory ameloblasts - tooth development


Publication History

Article published online:
19 September 2024

© 2024. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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