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CC BY-NC-ND 4.0 · Indian J Plast Surg
DOI: 10.1055/s-0044-1787561
Original Article

Effect of Basic Fibroblast Growth Factor in Perifascial Areolar Tissue Transplant

Junya Oshima
1   Department of Plastic, Reconstructive, and Hand Surgery, University of Tsukuba, 1-1-1Tennodai, Tsukuba, Ibaraki, Japan
,
Yoichiro Shibuya 
1   Department of Plastic, Reconstructive, and Hand Surgery, University of Tsukuba, 1-1-1Tennodai, Tsukuba, Ibaraki, Japan
,
Kaoru Sasaki 
1   Department of Plastic, Reconstructive, and Hand Surgery, University of Tsukuba, 1-1-1Tennodai, Tsukuba, Ibaraki, Japan
,
Mitsuru Sekido
1   Department of Plastic, Reconstructive, and Hand Surgery, University of Tsukuba, 1-1-1Tennodai, Tsukuba, Ibaraki, Japan
› Author Affiliations Funding The authors disclose receipt of the following partial financial support for the research, authorship, and/or publication of this article: JSPS Kakenhi, grant number 23K08419.
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Abstract

Background: Perifascial areolar tissue (PAT) transplant is a technique in which a sheet of connective tissue on the fascia is harvested and transplanted to the wound bed. PAT engraftment fails when the exposed area of tendons, bones, or artificial materials is large. On the other hand, combination of tissue transplant and basic fibroblast growth factor (bFGF) improves the survival rate of the transplanted tissue.

Methods: A wound model was created in which the artificial material was exposed on rats' backs. All the rats underwent PAT transplant, but the rats were divided into two groups according to the PAT processing method beforehand. In one group, the PAT was immersed in water for injection before transplant (bFGF[–] group), and in the other group, the PAT was immersed in bFGF product (bFGF[+] group). Specimens were collected 7 days after surgery to assess the histologic thickness of the PAT and the gene expression in the PAT.

Results: The thickness of the PAT in the tissue slices was significantly higher in the bFGF(+) group than in the bFGF(–) group. Expressions of CD34 and COL3A1 were significantly higher in the bFGF(+) group than in the bFGF(–) group.

Conclusion: The results of this study indicate that adding bFGF to the PAT transplant may promote PAT engraftment and wound healing by increasing angiogenesis and may increase granulation formation, which may result in a stronger covering that prevents the prosthesis from being exposed.

Keywords

perifascial areolar tissue - loose connective tissue - basic fibroblast growth factor - ulcer

Ethical Approval

All the study procedures were performed in accordance with the guidelines of our institutional animal care and use committee (approval number 2021-101).


Authors' Contributions

J.O. conceived the idea of the study. Y.S. developed the statistical analysis plan and conducted the statistical analyses. J.O. drafted the original manuscript. K.S. and M.S. supervised the conduct of the study. All the authors reviewed the manuscript draft and revised it critically for intellectual content. All the authors approved the final version of the manuscript.




Publication History

Article published online:
12 June 2024

© 2024. Association of Plastic Surgeons of India. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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