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Horm Metab Res 2018; 50(04): 325-330
DOI: 10.1055/s-0044-101834
Endocrine Research
© Georg Thieme Verlag KG Stuttgart · New York

Thyroid-Stimulating Hormone-Stimulated Human Adipocytes Express Thymic Stromal Lymphopoietin

Loretta Ma
1   Chronic Disease Program, Ottawa Hospital Research Institute, University of Ottawa, Ottawa, ON, Canada
,
AnneMarie Gagnon
1   Chronic Disease Program, Ottawa Hospital Research Institute, University of Ottawa, Ottawa, ON, Canada
,
Anne Landry
1   Chronic Disease Program, Ottawa Hospital Research Institute, University of Ottawa, Ottawa, ON, Canada
,
Timothea Le
1   Chronic Disease Program, Ottawa Hospital Research Institute, University of Ottawa, Ottawa, ON, Canada
,
Fengxia Xiao
1   Chronic Disease Program, Ottawa Hospital Research Institute, University of Ottawa, Ottawa, ON, Canada
,
Cathy Sun
2   Department of Medicine, University of Ottawa, Ottawa, ON, Canada
,
Heather A. Lochnan
1   Chronic Disease Program, Ottawa Hospital Research Institute, University of Ottawa, Ottawa, ON, Canada
2   Department of Medicine, University of Ottawa, Ottawa, ON, Canada
,
Dylan Burger
1   Chronic Disease Program, Ottawa Hospital Research Institute, University of Ottawa, Ottawa, ON, Canada
3   Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada
,
Alexander Sorisky
1   Chronic Disease Program, Ottawa Hospital Research Institute, University of Ottawa, Ottawa, ON, Canada
2   Department of Medicine, University of Ottawa, Ottawa, ON, Canada
4   Department of Biochemistry, Microbiology & Immunology, University of Ottawa, Ottawa, ON, Canada
› Author Affiliations
Further Information

Publication History

received 17 July 2017

accepted 23 January 2018

Publication Date:
19 February 2018 (online)

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Abstract

When recombinant human (rh) thyroid-stimulating hormone (TSH) is administered to thyroid cancer survivors, an acute extra-thyroidal effect raises pro-inflammatory cytokines and activates platelets. Thymic stromal lymphopoietin (TSLP) is a cytokine recently implicated in platelet activation. Our aim was to measure platelet microparticle levels after rhTSH stimulation in vivo, and to investigate TSLP expression in TSH-stimulated human adipocytes in culture. Blood samples for total and platelet microparticle analysis were obtained from thyroid cancer survivors before (day 1) and after rhTSH administration (day 5). Adipocytes, differentiated from stromal preadipocytes isolated from adipose tissue from surgical patients, were stimulated with TSH. TSLP mRNA expression, protein expression, and protein release into the adipocyte medium were measured. The level of platelet microparticles in thyroid cancer patients rose 5-fold after rhTSH stimulation. TSH upregulated TSLP mRNA expression in adipocytes in culture through a pathway that was inhibited by 66% by H89, a protein kinase A inhibitor. TSLP protein expression rose in response to TSH, and TSH-stimulated TSLP release into the medium was completely blocked by dexamethasone. In conclusion, TSLP is a novel TSH-responsive adipokine. Future studies will be needed to address the potential role of adipocyte-derived TSLP and whether it is linked to TSH-dependent platelet activation.

Key words

cytokine - platelet - microparticles - protein kinase A
 

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