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Thromb Haemost 2024; 124(03): 192-202
DOI: 10.1055/s-0043-1776305
Cellular Haemostasis and Platelets

Platelet Function and Maturity and Related microRNA Expression in Whole Blood in Patients with ST-Segment Elevation Myocardial Infarction

Oliver Buchhave Pedersen 
1   Thrombosis and Haemostasis Research Unit, Department of Clinical Biochemistry, Aarhus University Hospital, Aarhus, Denmark
2   Department of Cardiology, Aarhus University Hospital, Aarhus, Denmark
3   Department of Clinical Medicine, Faculty of Health, Aarhus University, Aarhus, Denmark
,
Anne-Mette Hvas
4   Faculty of Health, Aarhus University, Aarhus, Denmark
,
Leonardo Pasalic
5   Institute of Clinical Pathology and Medical Research, Westmead Hospital, NSW Health Pathology, Sydney, Australia
6   Westmead Clinical School, Faculty of Medicine and Health, University of Sydney, Sydney, Australia
,
Steen Dalby Kristensen
2   Department of Cardiology, Aarhus University Hospital, Aarhus, Denmark
3   Department of Clinical Medicine, Faculty of Health, Aarhus University, Aarhus, Denmark
,
Erik Lerkevang Grove 
2   Department of Cardiology, Aarhus University Hospital, Aarhus, Denmark
3   Department of Clinical Medicine, Faculty of Health, Aarhus University, Aarhus, Denmark
,
Peter H. Nissen
1   Thrombosis and Haemostasis Research Unit, Department of Clinical Biochemistry, Aarhus University Hospital, Aarhus, Denmark
3   Department of Clinical Medicine, Faculty of Health, Aarhus University, Aarhus, Denmark
› Author Affiliations Funding We received financial support from the Department of Clinical Biochemistry, Aarhus University Hospital, Denmark, Snedkermester Sophus Jacobsen and hustru Astrid Jacobsens Foundation, Murermester Lauritz Peter Christensen og hustru Kirsten Sigrid Christensens Foundation, A.P. Moeller og Hustru Chastine Mc-Kinney Møllers Fond til almene Formaal (A.P. Moellers Foundation), Fabrikant Karl G. Andersens Foundation, Helga og Peter Kornings Foundation, Familien Hede Nielsens Foundation, Danish Cardiovascular Academy, Overlæge Johan Boserup og Lise Boserups Legat, Carl og Ellen Hertz legat til Dansk Læge- og Naturvidenskab, Torben og Alice Frimodts Foundation, Arvid Nilssons Foundation, Direktør Emil C Hertz og Hustru Inger Hertz' Foundation, Raimond og Dagmar Ringgård-Bohns Foundation, Eva og Henry Frænkels Mindefond, Jørgen Møllers Foundation, and Frimodt-Heineke Foundation. The funding sources had no influence on the study.
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Abstract

Background Reduced effect of antiplatelet therapy has been reported in patients with ST-segment elevation myocardial infarction (STEMI). MicroRNAs (miRs) may influence platelet function and maturity, and subsequently the effect of antiplatelet therapy.

Objectives We aimed to explore the association between miR expression and platelet function and maturity in patients with acute STEMI and healthy individuals.

Methods We performed an observational study of STEMI patients admitted directly to primary percutaneous coronary intervention. Patients were treated with antiplatelet therapy according to guidelines. Within 24 hours after admission, blood samples were obtained to measure: the expression of 10 candidate miRs, platelet function markers using advanced flow cytometry, platelet aggregation, serum thromboxane B2, and platelet maturity markers. Furthermore, blood samples from healthy individuals were obtained to determine the normal variation.

Results In total, 61 STEMI patients and 50 healthy individuals were included. STEMI patients had higher expression of miR-21–5p, miR-26b-5p, and miR-223–3p and lower expression of miR-150–5p, miR423–5p, and miR-1180–3p than healthy individuals. In STEMI patients, the expression of miR-26b-5p showed the most consistent association with platelet function (all p-values <0.05, Spearman's rho ranging from 0.27 to 0.41), while the expression of miR-150–5p and miR-223–3p showed negative associations with platelet function. No association between miR expression and platelet maturity markers was observed.

Conclusion In patients with STEMI, the expression of six miRs was significantly different from healthy individuals. The expression of miR-26b-5p may affect platelet function in acute STEMI patients and potentially influence the effect of antiplatelet therapy.

Keywords

platelets - microRNAs - myocardial infarction - platelet function tests - platelet maturity

Authors' Contribution

All authors designed the research study; O.B.P. collected the data and performed the first data analysis; all authors contributed to interpretation of data and writing the article.


Supplementary Material



Publication History

Received: 24 July 2023

Accepted: 29 September 2023

Article published online:
16 October 2023

© 2023. Thieme. All rights reserved.

Georg Thieme Verlag KG
Rüdigerstraße 14, 70469 Stuttgart, Germany

 

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