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CC BY 4.0 · Eur J Dent 2024; 18(03): 841-851
DOI: 10.1055/s-0043-1776118
Original Article

Hyperbaric Oxygen Therapy to Minimize Orthodontic Relapse in Rabbits

Yun Mukmin Akbar 
1   Faculty of Dentistry, Universitas Padjadjaran, Bandung, Indonesia
2   Department of Research and Development, Indonesian Naval Dental Institute R.E. Martadinata, Jakarta, Indonesia
,
Ani Melani Maskoen
3   Department of Oral Biology, Faculty of Dentistry, Universitas Padjadjaran, Bandung, Indonesia
,
Endah Mardiati
4   Department of Orthodontics, Faculty of Dentistry, Universitas Padjadjaran, Bandung, Indonesia
,
Ganesha Wandawa
2   Department of Research and Development, Indonesian Naval Dental Institute R.E. Martadinata, Jakarta, Indonesia
,
Amaliya Amaliya
5   Departement of Periodontology, Faculty of Dentistry, Universitas Padjadjaran, Bandung, Indonesia
6   Center Study for Military Dentistry, Faculty of Dentistry, Universitas Padjadjaran, Bandung, Indonesia
,
Ida Bagus Narmada
7   Department of Orthodontics, Faculty of Dentistry, Universitas Airlangga, Surabaya, Indonesia
,
Nina Djustiana
8   Department of Dental Material, Faculty of Dentistry, Universitas Padjadjaran, Bandung, Indonesia
,
Ida Ayu Evangelina
4   Department of Orthodontics, Faculty of Dentistry, Universitas Padjadjaran, Bandung, Indonesia
,
Rasmi Rikmasari
9   Department of Prosthodontics, Faculty of Dentistry, Universitas Padjadjaran, Bandung, Indonesia
,
Mas Rizky Anggun
10   Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia
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Abstract

Objectives The purpose of the present study was to discover how hyperbaric oxygen therapy (HBOT) could reduce orthodontic relapse by altering the expressions of hypoxia-inducible factor (HIF)-1 messenger ribonucleic acid (mRNA), type I collagen (Col I), and matrix metalloproteinase-1 (MMP-1) in the gingival supracrestal fibers in rabbits.

Materials and Methods This study involved 44 male rabbits (Oryctolagus cuniculus) randomly divided into the normal group (K0), the orthodontic group without HBOT (K1), and the orthodontic group with HBOT (K2). Following orthodontic separation of the two upper central incisors, a retention phase and relapse assessment were performed. The HBOT was performed for a period of 2, 4, 6, 8, and 10 days after retention. HIF-1α transcription was assessed employing real-time polymerase chain reaction, whereas Col I and MMP-1 proteins were examined using immunohistochemistry. The orthodontic relapse was measured clinically using a digital caliper.

Statistical Analysis We used the one-way analysis of variance followed by Tukey's post hoc for multiple comparisons to measure differences between pairs of means; a p-value of 0.05 was considered statistically significant.

Results HBOT significantly increased the HIF-1α mRNA expression (p = 0.0140), increased Col I (p = 0.0043) and MMP-1 (p = 0.0068) on the tensioned and pressured side of the gingival supracrestal fibers, respectively, and clinically decreased the relapse (p = 3.75 × 10−40).

Conclusion HBOT minimizes orthodontic relapse by influencing HIF-1α expression, collagen synthesis (Col I), and degradation (MMP-1). This result suggests that HBOT has the potential to be used as an adjunctive method in the orthodontic retention phase.

Keywords

hyperbaric oxygen therapy - orthodontic relapse - collagen synthesis and degradation - supracrestal gingival fibers - animal model


Publication History

Article published online:
10 January 2024

© 2024. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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