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CC BY 4.0 · Chinese medicine and natural products 2023; 03(03): e133-e142
DOI: 10.1055/s-0043-1773796
Original Article

Research on the Effects of Neuroglobin on Ferroptosis in the Nerve Cells

Wenjin Gao
1   School of Basic Medicine and Forensic Medicine, Henan University of Science and Technology, Luoyang, Henan, China
,
Chen Mo
1   School of Basic Medicine and Forensic Medicine, Henan University of Science and Technology, Luoyang, Henan, China
,
Wei Feng
1   School of Basic Medicine and Forensic Medicine, Henan University of Science and Technology, Luoyang, Henan, China
,
Xinmin Pan
1   School of Basic Medicine and Forensic Medicine, Henan University of Science and Technology, Luoyang, Henan, China
,
Haojie Qin
1   School of Basic Medicine and Forensic Medicine, Henan University of Science and Technology, Luoyang, Henan, China
› Author Affiliations Funding This work was funded by Henan Science and Technology Research Program Project (222102310398).
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Abstract

Objectives The objective of this article was to explore the effects of neuroglobin (NGB) on ferroptosis in the nerve cells.

Methods The NGB knockdown model of HT22 cells was constructed, and the ferroptosis-related indexes of cell proliferation activity, contents of iron ion, malondialdehyde (MDA), superoxide and reactive oxygen, and the changes of nuclear factor E2-related factor 2 (Nrf2) expression were examined in the normal group, erastin group, NGB siRNA group, and NGB siRNA + Erastin group, respectively.

Results Compared with the normal group, cell proliferation activity and Nrf2 expression were significantly lower in the erastin group, NGB siRNA group, and NGB siRNA + erastin group, and ferroptosis-related indexes such as iron ion content, MDA content, superoxide content, and reactive oxygen species content were significantly reduced, and the difference between NGB siRNA + erastin group, erastin group, and NGB siRNA group was statistically significant (p < 0.05).

Conclusion Knockdown of NGB in cells enhances the action of the ferroptosis inducer erastin, and NGB may regulate the cellular ferroptosis process through Nrf2. This research may provide references for the clinical treatment of nervous system disease with Chinese medicine.

Keywords

neuroglobin - HT22 cells - ferroptosis - siRNA - nerve cells

CRediT Authorship Contribution Statement

W.G. was responsible for data curation, visualization, software, and writing—original draft. C.M. was responsible for conceptualization, data curation, formal analysis, and writing—original draft. W.F. was responsible for investigation, data curation, and project administration. X.P. was responsible for writing—review and editing and formal analysis. H.Q. was responsible for conceptualization, methodology, funding acquisition, supervision, and writing-review & editing.




Publication History

Received: 20 March 2023

Accepted: 08 May 2023

Article published online:
27 September 2023

© 2023. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

Georg Thieme Verlag KG
Rüdigerstraße 14, 70469 Stuttgart, Germany

 

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