PDF icon PDF herunterladen
Open Access
CC BY 4.0 · Eur J Dent 2024; 18(02): 672-679
DOI: 10.1055/s-0043-1772779
Original Article

Assessment of Salivary MMP-8 and IL-1β for the Diagnosis of Periodontal Diseases in Pakistani Population

Rida Kainat
1   Department of Biochemistry, Baqai Medical University, Karachi, Pakistan
,
Iftikhar Ahmed
1   Department of Biochemistry, Baqai Medical University, Karachi, Pakistan
,
Abdullah Mohammed Alolaywi
2   Dental Department, Hotat Bani Tamim General Hospital, Ministry of Health, Riyad, Saudi Arabia
,
Humera Waheed
3   Dow College of Biotechnology, Dow University of Health Sciences, Karachi, Pakistan
,
Zohaib Khurshid Sultan 
4   Department of Prosthodontics and Dental Implantology, College of Dentistry, King Faisal University, Al-Ahsa, Saudia Arabia
5   Department of Anatomy, Faculty of Dentistry, Center of Excellence for Regenerative Dentistry, Chulalongkorn University, Bangkok, Thailand
,
Syed Faraz Moin 
6   Dr. Zafar H. Zaidi Center for Proteomics, University of Karachi, Karachi, Pakistan
› Institutsangaben
Funding This study was supported by grant of Baqai Medical University, Department of Biochemistry.
› Weitere Informationen
Auch verfügbar aufeRef
  Lizenzen und Reprints
Preview

Abstract

Objective Clinical methods use the subjective diagnosis of periodontal diseases by visual observation that could result in differences and variability of diagnosis. The addition of specific markers could aid in the accurate diagnosis of the local population. The objective of the study was to target two of the major proteins for possible significance in such an approach.

Materials and Methods Unstimulated saliva samples were collected from 60 participants aged between 18 and 70 years. Three groups each with twenty participants were recruited into periodontitis, gingivitis, and healthy control.

Statistical Analysis The samples were analyzed using human enzyme-linked immunosorbent assay kits for matrix metalloproteinase-8 (MMP-8) and interleukin-1β (IL-1β).

Results SPSS version 20 was used to analyze the result. Posthoc analysis by Tukey's test revealed that MMP-8 levels were higher in gingivitis and periodontitis groups as compared with healthy controls. The test also revealed that IL-1β levels were higher in the periodontitis group compared with the healthy control and gingivitis group. Additionally, one-way analysis of variance analysis showed a significant effect on probing depth in gingivitis and periodontitis patients. The mean age of periodontitis group was significantly higher than other groups.

Conclusion Salivary biomarkers may provide useful diagnostic information and could be utilized as tests for periodontal disease screening, prognosis, and prediction.

Keywords

saliva - biomarkers - MMP-8 - IL-1β - gingivitis - periodontal disease

Ethical Approval Statement

This study was approved by the Institutional Ethical Committee, Baqai Medical University Karachi (BASR, IBC # BMU-EC/02–2021) and an informed consent was obtained from each participant.




Publikationsverlauf

Artikel online veröffentlicht:
12. Dezember 2023

© 2023. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

Thieme Medical and Scientific Publishers Pvt. Ltd.
A-12, 2nd Floor, Sector 2, Noida-201301 UP, India