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CC BY 4.0 · Eur J Dent 2024; 18(02): 501-509
DOI: 10.1055/s-0043-1772699
Original Article

Gingiva Mesenchymal Stem Cells Normoxic or Hypoxic Preconditioned Application Under Orthodontic Mechanical Force on Osterix, Osteopontin, and ALP Expression

Alexander Patera Nugraha
1   Department of Orthodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Ida Bagus Narmada
1   Department of Orthodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Ervina Restiwulan Winoto
1   Department of Orthodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
I Gusti Aju Wahju Ardani
1   Department of Orthodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Ari Triwardhani
1   Department of Orthodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Alida Alida
1   Department of Orthodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Adya Pramusita
1   Department of Orthodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Reyhan Mahendra Nur
1   Department of Orthodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Nuraini Indrastie
1   Department of Orthodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Hui Yin Nam
2   Nanotechnology and Catalysis Research Centre (NANOCAT), Universiti Malaya, Kuala Lumpur, Malaysia
3   Tissue Engineering Group, Department of Orthopaedic Surgery (NOCERAL), Faculty of Medicine, Universiti Malaya, Kuala Lumpur, Malaysia
,
Igo Syaiful Ihsan
4   Stem Cell Research and Development Center, Universitas Airlangga, Surabaya, Indonesia
,
Wibi Riawan 
5   Biomolecular Biochemistry, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
,
Fedik Abdul Rantam
6   Laboratory of Immunology and Virology Department of Microbiology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Albertus Putera Nugraha 
7   Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Tengku Natasha Eleena binti Tengku Ahmad Noor
8   Membership of Faculty of Dental Surgery, Royal Collage of Surgeon, Edinburgh University, United Kingdom
9   Malaysian Armed Forces Dental Officer, 609 Armed Forces Dental Clinic, Kem Semenggo, Kuching, Sarawak, Malaysia
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Abstract

Objectives The aim of this article was to investigate Osterix, ALP, and osteopontin expression in the compression and tension sides of alveolar bone after the application of normoxic/hypoxic-preconditioned GMSCs in rabbits (Oryctolagus cuniculus) induced with OMF.

Materials and Methods Forty-eight healthy, young male rabbits were divided into four groups: [-] OMF; [+] OMF; OMF with GMSCs normoxic-preconditioned; and OMF and GMSCs hypoxic-preconditioned. The central incisor and left mandibular molar in the experimental animals were moved, the mandibular first molar was moved mesially using nickel titanium (NiTi) and stainless steel ligature wire connected to a 50 g/mm2 light force closed coil spring. Allogeneic application of normoxic or hypoxic-preconditioned GMSCs was used in as many as 106 cells in a 20 µL phosphate buffered saline single dose and injected into experimental animals' gingiva after 1 day of OTM. On days 7, 14, and 28, all experimental animals were euthanized. Osterix, ALP, and osteopontin expressions were examined by immunohistochemistry.

Results Osterix, ALP, and osteopontin expressions were significantly different after allogeneic application of hypoxic-preconditioned GMSCs than normoxic-preconditioned GMSCs in the tension and compression of the alveolar bone side during OMF (p < 0.05).

Conclusion Osterix, ALP, and osteopontin expressions were significantly more enhanced post-transplantation of GMSCs with hypoxic-preconditioning than after transplantation of normoxic-preconditioned GMSCs in rabbits (O. cuniculus) induced with OMF.

Keywords

alveolar bone - hypoxia - medicine - orthodontic tooth movement - stem cells


Publication History

Article published online:
23 November 2023

© 2023. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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