Download PDF
CC BY-NC-ND 4.0 · J Lab Physicians 2023; 15(04): 552-557
DOI: 10.1055/s-0043-1768950
Original Article

Evaluation of Accuracy of Intraoperative Frozen Section and Imprint Cytology in Gynecological Neoplasms—A Descriptive Cross-Sectional Study of 50 Cases in Tertiary Care Center

Ayushi Kediya 
1   Department of Pathology, Himalayan Institute of Medical Sciences, Swami Rama Himalayan University, Dehradun, Uttarakhand, India
,
Nadia Shirazi 
1   Department of Pathology, Himalayan Institute of Medical Sciences, Swami Rama Himalayan University, Dehradun, Uttarakhand, India
,
Ruchira Nautiyal 
2   Department of Gynecology and Obstetrics, Himalayan Institute of Medical Sciences, Swami Rama Himalayan University, Dehradun, Uttarakhand, India
› Author Affiliations Financial Support None.
The study was approved by institutional review board.
› Further Information
   Permissions and Reprints

Abstract

Aims and Objectives Gynecological neoplasms are among the most common cancers in female population of India and worldwide. Various new advances have been made to diagnose gynecological pathologies which include imprint cytology and frozen sections in addition to the histopathological techniques. The aim of the study was to assess the diagnostic accuracy of intraoperative diagnostic procedure with gold standard histopathology.

Method The study included 50 patients who had suspected gynecological neoplasm in a time period of 12 months. Their intraoperative diagnosis was made on frozen section and imprint cytology to rule out benign or malignant lesions and compared with gold standard histopathology.

Results Our study concludes that maximum cases of female genital tract neoplasms belonged to the age group of 19 to 76 years, with 60% cases in postmenopausal age group. Overall diagnosis of 62, 52, and 76% malignancies were made on imprint cytology, frozen section, and histopathological examination, respectively.

Conclusion The study concludes that diagnostic accuracy by intraoperative imprint cytology is higher (80%) than frozen section (76%). True positive cases were maximally reported by histopathology. True negative and false positive cases were equally reported by both frozen and imprint cytology. False negative cases were reported by frozen section more than by imprint cytology. The kappa statistical value was lesser in frozen versus histopathology and more in imprint versus histopathology. The sensitivity, specificity, positive predictive value, and negative predictive value for imprint cytology were 77.5, 90.0, 96.9, and 50%, respectively, whereas for frozen section, it was 72.5, 90.0, 96.7, and 45.0%, respectively.

Keywords

frozen - imprint cytology - sensitivity

Authors' Contributions

Conceptualization: A.K., N.S.


Data curation: A.K.


Critical and intellectual evaluation: N.S., R.N., A.K.


Drafting of manuscript: A.K., N.S.


Approval of final manuscript: All authors.




Publication History

Received: 14 August 2022

Accepted: 04 April 2023

Article published online:
13 June 2023

© 2023. The Indian Association of Laboratory Physicians. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

Thieme Medical and Scientific Publishers Pvt. Ltd.
A-12, 2nd Floor, Sector 2, Noida-201301 UP, India

 

  • References

  • 1 Sung H, Ferlay J, Siegel RL. et al. Global Cancer Statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin 2021; 71 (03) 209-249
    CrossrefPubMedGoogle Scholar
  • 2 Dudgeon LS, Patrick CVR, Paolino M. A new method for the rapid microscopical diagnosis of tumours: with an account of 200 cases so examined. J. Brit. Surgery 1927; 15 (58) 250-261
    CrossrefGoogle Scholar
  • 3 Loncar B, Pajtler M, Milicić-Juhas V, Kotromanović Z, Staklenac B, Pauzar B. Imprint cytology in laryngeal and pharyngeal tumours. Cytopathology 2007; 18 (01) 40-43
    CrossrefPubMedGoogle Scholar
  • 4 Bokhman IaV, Nechaeva ID, Maksimov SIa, Kutusheva GF, Kolygin BA. Zlokachestvennye opukholi genitaliĭ u devochek. [Malignant tumors of the genitalia in girls] Vopr Onkol 1986; 32 (01) 74-80
    PubMedGoogle Scholar
  • 5 Lee TK. The value of imprint cytology in tumor diagnosis: a retrospective study of 522 cases in northern China. Acta Cytol 1982; 26 (02) 169-171
    PubMedGoogle Scholar
  • 6 Kumar M, Rajwanshi A, Dey P. Carcinoid of the ovary: diagnostic challenge on fine-needle aspiration cytology. Diagn Cytopathol 2014; 42 (07) 612-614
    CrossrefPubMedGoogle Scholar
  • 7 Terzic M, Dokic M, Stimec B. Immature ovarian teratoma in a young girl: very short course and lethal outcome. A case report. Int J Gynecol Cancer 2005; 15 (02) 382-384
    CrossrefPubMedGoogle Scholar
  • 8 Morice P, Wicart-Poque F, Rey A. et al. Results and fertility after conservative treatment of invasive epithelial ovarian cancer [in French]. Gynécol Obstét Fertil 2002; 30 (09) 684-691
    PubMedGoogle Scholar
  • 9 Platz CE, Benda JA. Female genital tract cancer. Cancer 1995; 75 (01) 270-294
    CrossrefPubMedGoogle Scholar
  • 10 Shahid M, Zaheer S, Mubeen A, Rahman K, Sherwani RK. The role of intraoperative cytology in the diagnostic evaluation of ovarian neoplasms. Acta Cytol 2012; 56 (05) 467-473
    CrossrefPubMedGoogle Scholar
  • 11 Khan AH, Mamoon N, Usman M, Laleka US, Malik B. Accuracy of intra-operative frozen section in the diagnosis of female genital tract neoplasms. J Pak Med Assoc 2016; 66 (02) 143-146
    PubMedGoogle Scholar
  • 12 Negri E, La Vecchia C, Bosetti C, Franceschi S, Parazzini F. Risk of cervical cancer in women with a family history of breast and female genital tract neoplasms. Int J Cancer 2005; 117 (05) 880-881
    CrossrefPubMedGoogle Scholar
  • 13 Cramer DW, Barbieri RL, Muto MG, Kelly A, Brucks JP, Harlow BL. Characteristics of women with a family history of ovarian cancer. II. Follicular phase hormone levels. Cancer 1994; 74 (04) 1318-1322
    CrossrefPubMedGoogle Scholar