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Exp Clin Endocrinol Diabetes 2019; 127(07): 477-484
DOI: 10.1055/s-0043-125315
Article
© Georg Thieme Verlag KG Stuttgart · New York

Combining Various Methods to Assess Insulin Sensitivity in Nonobese Rat after Sleeve Gastrectomy

Dong Sun
1   Department of General Surgery, Qilu Hospital of Shandong University, Jinan 250012, Shandong, People’s Republic of China
,
Yanmin Wang
2   Department of General Surgery, Qianfo Shan Hospital Affiliated to Shandong University, Jinan, 250014, Shandong, People’s Republic of China
,
Meng Wei
1   Department of General Surgery, Qilu Hospital of Shandong University, Jinan 250012, Shandong, People’s Republic of China
,
Zongli Zhang   *
1   Department of General Surgery, Qilu Hospital of Shandong University, Jinan 250012, Shandong, People’s Republic of China
,
Sanyuan Hu   *
1   Department of General Surgery, Qilu Hospital of Shandong University, Jinan 250012, Shandong, People’s Republic of China
› Author Affiliations
Further Information

Publication History

received 01 October 2017
revised 30 November 2017

accepted 21 December 2017

Publication Date:
08 February 2018 (online)

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Abstract

Background Sleeve gastrectomy (SG) procedure has been proved to improve insulin sensitivity and sustain anti-diabetic effects. Our aim is to co-use several methods to measure insulin sensitivity and investigate the effect of SG on hepatic and peripheral insulin sensitivity at early and long-term stages of postoperation.

Methods Thirty 11-week-old male Goto-Kakizaki rats were divided into SG, sham-operated SG (SOSG), and control groups. They were observed before operation and for 36 weeks of postoperation. Insulin tolerance test (ITT) and homeostasis model of assessment for insulin resistance index(HOMA-IR)were used to measure insulin resistance before operations and at 2 and 36 weeks of postoperation; Pyruvate challenge test (PCT) was administrated to assess the gluconeogenesis capability in order to reflect hepatic insulin sensitivity before operation and at 2 and 36 weeks of postoperation; Hyperinsulinemic euglycemic clamps (HIEC) was conducted before operation and at 2 and 36 weeks of postoperation to calculate the endogenous hepatic glucose production (HGP) at the basal and steady-state for evaluation of hepatic insulin sensitivity, and calculate the exogenous glucose infusion rate (GIR) at the steady-state for evaluation of peripheral insulin sensitivity.

Results The data showed that compared with rats in the sham and control groups, rats in SG group had 1) significantly lower AUCITT, HOMA-IR and AUC PCT values at 2 and 36 weeks of postoperation, 2) lower basal state HGP, but not steady-state GIR at 2 weeks of postoperation, and 3) significantly different basal and steady-state HGP and steady-state GIR at 36 weeks of postoperation. In addition, the basal and steady-state HGP and the steady-state GIR were significantly different between rats in SG group at 2 and 36 weeks of postoperation.

Conclusions This study explored insulin sensitivity of rats after SG by jointly using a variety of techniques. The results showed that SG time-dependently improved the hepatic and peripheral insulin sensitivity.

Key words

Sleeve gastrectomy - Insulin sensitivity - Goto-Kakizaki rats

* Sanyuan Hu and Zongli Zhang contributed equally to this work.


 

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