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CC BY-NC-ND 4.0 · Planta Medica International Open 2017; 4(01): e35-e42
DOI: 10.1055/s-0043-108760
Original Article
Georg Thieme Verlag KG Stuttgart · New York

Effect of Dichloromethane-Methanol Extract and Tomentin Obtained from Sphaeralcea angustifolia Cell Suspensions in a Model of Kaolin/Carrageenan-Induced Arthritis

María del Pilar Nicasio-Torres
1   Centro de Investigación Biomédica del Sur (CIBIS), Instituto Mexicano del Seguro Social (IMSS), Xochitepec, Morelos, México
,
Jade Serrano-Román
1   Centro de Investigación Biomédica del Sur (CIBIS), Instituto Mexicano del Seguro Social (IMSS), Xochitepec, Morelos, México
,
Juanita Pérez-Hernández
1   Centro de Investigación Biomédica del Sur (CIBIS), Instituto Mexicano del Seguro Social (IMSS), Xochitepec, Morelos, México
,
Enrique Jiménez-Ferrer
1   Centro de Investigación Biomédica del Sur (CIBIS), Instituto Mexicano del Seguro Social (IMSS), Xochitepec, Morelos, México
,
Maribel Herrera-Ruiz
1   Centro de Investigación Biomédica del Sur (CIBIS), Instituto Mexicano del Seguro Social (IMSS), Xochitepec, Morelos, México
› Author Affiliations
Further Information

Publication History

received 15 November 2016
revised 27 March 2017

accepted 28 March 2017

Publication Date:
23 May 2017 (online)

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Abstract

Cell suspension cultures from Sphaeralcea angustifolia produce compounds with anti-inflammatory activity, including scopoletin, tomentin, and sphaeralcic acid. Antiarthritic activity of scopoletin isolated from S. angustifolia and Erycibe obtusifolia wild plants was demonstrated in a complete Freud’s adjuvant-induced arthritis model. In this project, the dichloromethane-methanol extract and tomentin isolated from S. angustifolia cell suspension were evaluated in mice using a kaolin/carrageenan-induced arthritis model. After 9 days of treatment, the anti-inflammatory effect of S. angustifolia dichloromethane-methanol extract was 72% at a dose of 100 mg/kg, a recovery of 55% was observed with methotrexate (5.0 mg/kg), and the anti-inflammatory effect for tomentin was dose-dependent with a median effective dose of 10.32 mg/kg. The concentration of pro-inflammatory cytokines interleukin-1β and tumor necrosis factor alpha in the left joint of groups treated with S. angustifolia dichloromethane-methanol extract and tomentin at different doses was significantly lower than that detected in the group damaged with kaolin/carrageenan, and the concentration of interleukin-10 and interleukin-4 (anti-inflammatory cytokines) was significantly higher. The drop in mean body weight of mice treated with methotrexate (−4.34±0.47 g) was higher in comparison with the kaolin/carrageenan group (−2.40±0.29 g). On the other hand, the mean body weight of mice treated with S. angustifolia dichloromethane-methanol extract (−1.19±0.17 g) was similar, and mice treated with tomentin (0.19±0.11 g) was lower. The dichloromethane-methanol extract and tomentin isolated from S. angustifolia diminished joint edema induced by kaolin/carrageenan, possibly by acting as an immunomodulatory of the inflammatory response.

Key words

Sphaeralcea angustifolia - Malvaceae - anti-inflammatory - arthritis - coumarins - scopoletin.

Supporting Information

    1H NMR spectra of tomentin, HPLC chromatograms of SaDM extract, tomentin, and sphaeralcic acid, and a curve of tomentin doses and joint edema size are available online at http://www.thieme-connect.de/products.
  • Supporting Information
 

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