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Drug Res (Stuttg) 2017; 67(08): 458-465
DOI: 10.1055/s-0043-106051
Original Article
© Georg Thieme Verlag KG Stuttgart · New York

Preparation and Characterization of Copolymeric Polymersomes for Protein Delivery

Alireza Nomani
1   Department of pharmaceutical biomaterials, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran
,
Hamed Nosrati
1   Department of pharmaceutical biomaterials, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran
,
Hamidreza Kheiri Manjili
2   Department of Pharmaceutical Nanotechnology, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran
3   Pharmaceutical Biotechnology Department, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran
,
Leila Khesalpour
1   Department of pharmaceutical biomaterials, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran
,
Hossein Danafar
4   Cancer Gene Therapy Research Center, Zanjan University of Medical Sciences, Zanjan, Iran
5   Zanjan Pharmaceutical Nanotechnology Research Center, Zanjan University of Medical Sciences, Zanjan, Iran
› Author Affiliations
Further Information

Publication History

received 31 December 2016

accepted 15 March 2017

Publication Date:
30 May 2017 (online)

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Abstract

Biodegradable copolymeric polymersomes have been used for controlled drug delivery of proteins. These polymersomes important areas to overcome formulation associated problems of the proteins. The aim of this study was to develop polymersomes using biodegradable copolymers for delivery of bovine serum albumin (BSA) as a model protein. Encapsulated BSA by mPEG-PCL polymersomes led to formation of BSA-loaded mPEG-PCL polymersomes. The polymersomes synthesized with the protein-polymer ratio of 1:4 at 15 000 rpm gave maximum loading, minimum polydispersion with maximally sustained protein release pattern, among the prepared polymersomes. Investigation on FTIR and DSC results revealed that such a high encapsulation efficiency is due to strong interaction between BSA and the copolymer.The particles size and their morphology of polymersomes were determined by DLS and AFM.The encapsulation efficiency of BSA was 91.02%. The results of AFM showed that the polymersomes had spherical shapes with size of 49 nm.The sizes of BSA-loaded polymersomes ranged from 66.06 nm to 84.97 nm. The results showed that polymersomes exhibited a triphasic release, for BSA. Overall, the results indicated that mPEG–PCL polymersomes can be considered as a promising carrier for proteins.

Key words

BSA - copolymers - sustained release - polymrsomes
 

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