Planta Med 2022; 88(15): 1511
DOI: 10.1055/s-0042-1759184
Poster Session II

Optimization of distillation process parameters of oregano by determining bioactive aroma constituents and in vitro antioxidant activity

A Nakas∗
1   Laboratory of Organic Chemistry, School of Chemical Engineering, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece
3   Natural Products Research Centre of Excellence, Center for Interdisciplinary Research and Innovation of Aristotle University of Thessaloniki, 57001, Thessaloniki, Greece
,
A Peperidou
2   Department of Pharmaceutical Chemistry, School of Pharmacy, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece
,
I Fotopoulos
2   Department of Pharmaceutical Chemistry, School of Pharmacy, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece
,
E Chainoglou
2   Department of Pharmaceutical Chemistry, School of Pharmacy, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece
,
G Giannarelli
1   Laboratory of Organic Chemistry, School of Chemical Engineering, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece
,
K N Kontogiannopoulos
1   Laboratory of Organic Chemistry, School of Chemical Engineering, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece
3   Natural Products Research Centre of Excellence, Center for Interdisciplinary Research and Innovation of Aristotle University of Thessaloniki, 57001, Thessaloniki, Greece
,
A Tsiaprazi-Stamou
4   Vessel Essential Oils, Neo Rysio, 57001, Thessaloniki, Greece
,
V Varsamis
4   Vessel Essential Oils, Neo Rysio, 57001, Thessaloniki, Greece
,
H Gika
3   Natural Products Research Centre of Excellence, Center for Interdisciplinary Research and Innovation of Aristotle University of Thessaloniki, 57001, Thessaloniki, Greece
5   School of Medicine, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece
,
D Hadjipavlou-Litina
2   Department of Pharmaceutical Chemistry, School of Pharmacy, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece
,
A Ν Assimopoulou
1   Laboratory of Organic Chemistry, School of Chemical Engineering, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece
3   Natural Products Research Centre of Excellence, Center for Interdisciplinary Research and Innovation of Aristotle University of Thessaloniki, 57001, Thessaloniki, Greece
› Institutsangaben
› Weitere Informationen
Auch verfügbar auf
 

Origanum vulgare (commonly known as oregano) is a widely used aromatic herb of the Lamiaceae family that has been growing in the Mediterranean region since antiquity. Oregano plants exhibit antioxidant and antimicrobial properties and have great economic value for perfumery, agricultural and food industry [1], [2].

Aim of the present work was to optimize the conditions of the distillation process that maximize the yield of specific bioactive compounds of the essential oil (EO) of oregano cultivated in Greece. Steam distillation was conducted at a pilot scale at the industrial distillery “Vessel Essential Oils” and the selected parameters of the implemented Face-Centered Composite experimental design were: time, temperature and pressure.

EOsʼ composition was determined by Headspace GC-MS analysis. The in vitro antioxidant activity of EOs from all samples was measured in terms of the interaction with the stable free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) after 20 and 60 min [3], [4]. Most prominent EOs were also tested as inhibitors of lipid peroxidation.

With the applied GC-MS method, more than 20 volatile compounds were identified in the oreganoʼs EOs. Data processing showed that the factor that mainly affects yield, is distillation time. The desired space was determined by conducting three-dimensional response surface analysis of the independent and dependent variables, choosing yields of thymol and carvacrol as optimization criteria.

This research has been co-financed by the European Union and Greek national funds through the Operational Program Competitiveness, Entrepreneurship and Innovation, under the call RESEARCH–CREATE–INNOVATE (project code: T1EDK-04174).



Publikationsverlauf

Artikel online veröffentlicht:
12. Dezember 2022

© 2022. Thieme. All rights reserved.

Georg Thieme Verlag KG
Rüdigerstraße 14, 70469 Stuttgart, Germany

 

  • References

  • 1 Lukas B, Schmiderer C, Novak J. Essential oil diversity of European Origanum vulgare L. (Lamiaceae). Phytochem 2015; 119: 32-40
    CrossrefPubMedSuche in Google Scholar
  • 2 Assiri AMA, Elbanna K, Al-Thubiani A, Ramadan MF. Cold-pressed oregano (Origanum vulgare) oil: a rich source of bioactive lipids with novel antioxidant and antimicrobial properties. Eur Food Res Technol 2016; 242: 1013-1023
    CrossrefPubMedSuche in Google Scholar
  • 3 Kouzi O, Pontiki E, Hadjipavlou-Litina D. 2-Arylidene-1-indandiones as Pleiotropic Agents with Antioxidant and Inhibitory Enzymes Activities. Molecules 2019; 24: 4411
    CrossrefPubMedSuche in Google Scholar
  • 4 Pontiki E, Hadjipavlou-Litina D. Multi-Target Cinnamic Acids for Oxidative Stress and Inflammation: Design, Synthesis, Biological Evaluation and Modeling Studies. Molecules 2018; 24: 12
    CrossrefPubMedSuche in Google Scholar