Planta Med 2022; 88(15): 1475
DOI: 10.1055/s-0042-1759101
Poster Session I

Recycling Antibiotics with Natural Substances as Adjuvants using Pseudomonas aeruginosa and Klebsiella pneumoniae as Examples

H Sakr
1   Free University of Berlin, Institute of Pharmacy, Königin-Luise-Str. 2 u. 4, 14195 Berlin, Germany
,
S Schmidt∗
2   Hofmann & Sommer GmbH u. Co. KG, Johann-Hittorf-Str. 8, 12489 Berlin, Germany
,
S Bereswill
3   Charité – Universitätsmedizin Berlin, corporate member of Free University of Berlin and Humboldt University of Berlin, Institute of Microbiology, Infectious Diseases and Immunology, Hindenburgdamm 30, 12203 Berlin, Germany
,
M M Heimesaat
3   Charité – Universitätsmedizin Berlin, corporate member of Free University of Berlin and Humboldt University of Berlin, Institute of Microbiology, Infectious Diseases and Immunology, Hindenburgdamm 30, 12203 Berlin, Germany
,
M F Melzig
1   Free University of Berlin, Institute of Pharmacy, Königin-Luise-Str. 2 u. 4, 14195 Berlin, Germany
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The development of resistance to antibiotics in bacterial pathogens is a threatening circumstance worldwide. The resulting need for effective therapeutics cannot be met by antibiotics alone [1]. One strategy to counter this threat is to combine antibiotics to which resistance already exists with natural products that enhance the antibiotic activity through resistance-modifying properties.

The purpose of this study was to increase the efficacy of the antibiotics by combining them with natural substances, so that even resistant bacteria react sensitively again. The resistance-decreasing effects of clove and cinnamon oil as well as lysozyme with carbapenem antibiotics (imipenem) and aminoglycosides (gentamicin) against the bacterial pathogens Pseudomonas aeruginosa and Klebsiella pneumoniae were investigated using checkerboard microdilution methods.

The essential oil of either plant species combined with lysozyme significantly increased the antibiotic effect. The minimum inhibitory concentrations (MICs) of gentamicin and imipenem against multidrug-resistant clinical isolates of the two Gram-negative bacterial species were reduced. For example, the MIC of gentamicin in a P. aeruginosa isolate was reduced from 13 107 (± 4487) mg/L to 32 mg/L by adding cinnamon oil and lysozyme (FICI 0.27).

The results show that the phenylpropanoid derivatives in both essential oils, in combination with lysozyme, are suitable for the development of new resistance-modifying substances against bacterial infections. Further work on resistance modification by these agents focuses on the analysis of as many clinical bacterial isolates as possible. In addition, the resistance-modifying properties could be further improved through chemical modification.



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Artikel online veröffentlicht:
12. Dezember 2022

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