CC BY 4.0 · Eur J Dent 2023; 17(03): 828-833
DOI: 10.1055/s-0042-1756689
Original Article

Methylene Blue Penetration of Resin Infiltration and Resin Sealant in Artificial White-Spot Lesions

1   Division of Restorative Dentistry, Faculty of Dentistry, Thammasat University, Pathumthani, Thailand
2   Thammasat University Research Unit in Restorative and Esthetic Dentistry, Thammasat University, Pathumthani, Thailand
,
Jarukit Vongsang
3   Faculty of Dentistry, Thammasat University, Pathumthani, Thailand
,
Thanach leelaudom
3   Faculty of Dentistry, Thammasat University, Pathumthani, Thailand
,
Nantawan Krajangta
1   Division of Restorative Dentistry, Faculty of Dentistry, Thammasat University, Pathumthani, Thailand
2   Thammasat University Research Unit in Restorative and Esthetic Dentistry, Thammasat University, Pathumthani, Thailand
› Author Affiliations
Funding This study was funded and supported by the Faculty of Dentistry Research Fund and Thammasat University Research Unit in Restorative and Esthetic Dentistry, Thammasat University, Pathumthani, Thailand.

Abstract

Objective This study determined the potency of resin infiltrations and resin sealant in impeding microleakage on artificial white-spot lesions (AWL) by methylene blue penetration.

Materials and Methods Eighty AWL specimens were randomly separated into two groups for water storage for 24 hours (groups 1–4) and 20,000 cycles of thermocycling (TC) (groups 5–8). Each group was then separated into four subgroups (n = 10) based on the AWL surface treatments: (1) no Tx, (2) treated with resin infiltration (ICON, DMG, Hamburg, Germany), (3) treated with resin infiltration (Surface pre-reacted glass-ionomer (S-PRG) barrier coat, SHOFU, Kyoto, Japan), (4) treated with resin sealant (Clinpro sealant, 3M ESPE, Minnesota, United States). Nail varnish was covered to all samples, with the exception of a 4 × 4 mm2 patch on the buccal measurement region, which was subsequently submerged in a 2% methylene blue solution and cut into buccolingual sections. Stereomicroscope measurements were used to calculate methylene blue penetration scores.

Statistical Analysis The Kruskal–Wallis test and the Bonferroni post-hoc correction were performed to evaluate the data.

Results Application of resin infiltrants and resin sealant reduced microleakage in AWL by methylene penetration both before and after thermal cycling. In addition, group 6 (ICON + TC) and group 7 (S-PRG + TC) had a significantly different value from group 8 (sealant + TC).

Conclusion Both the resin infiltration approach and the resin sealant seem to help seal AWL and might perhaps offer long-term defense against microleakage in AWL caused by methylene blue penetration. The greatest sealing and defense for microleakage in AWL were demonstrated by the resin infiltrations.



Publication History

Article published online:
11 October 2022

© 2022. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

Thieme Medical and Scientific Publishers Pvt. Ltd.
A-12, 2nd Floor, Sector 2, Noida-201301 UP, India

 
  • References

  • 1 Guzmán-Armstrong S, Chalmers J, Warren JJ. Ask us. White spot lesions: prevention and treatment. Am J Orthod Dentofacial Orthop 2010; 138 (06) 690-696
  • 2 Enaia M, Bock N, Ruf S. White-spot lesions during multibracket appliance treatment: a challenge for clinical excellence. Am J Orthod Dentofacial Orthop 2011; 140 (01) e17-e24
  • 3 Al-Khateeb S, Forsberg CM, de Josselin de Jong E, Angmar-Månsson B. A longitudinal laser fluorescence study of white spot lesions in orthodontic patients. Am J Orthod Dentofacial Orthop 1998; 113 (06) 595-602
  • 4 Mertz-Fairhurst EJ, Curtis Jr JW, Ergle JW, Rueggeberg FA, Adair SM. Ultraconservative and cariostatic sealed restorations: results at year 10. J Am Dent Assoc 1998; 129 (01) 55-66
  • 5 Simonsen RJ. Sealants and caries. J Am Dent Assoc 2013; 144 (07) 767-768
  • 6 Haricharan PB, Barad N, Patil CR, Voruganti S, Mudrakola DP, Turagam N. Dawn of a new age fissure sealant? A study evaluating the clinical performance of Embrace WetBond and ART sealants: results from a randomized controlled clinical trial. Eur J Dent 2019; 13 (04) 503-509
  • 7 Jodkowska E. Efficacy of pit and fissure sealing: long-term clinical observations. Quintessence Int 2008; 39 (07) 593-602
  • 8 Erdemir U, Sancakli HS, Yaman BC, Ozel S, Yucel T, Yıldız E. Clinical comparison of a flowable composite and fissure sealant: a 24-month split-mouth, randomized, and controlled study. J Dent 2014; 42 (02) 149-157
  • 9 Nahvi A, Razavian A, Abedi H, Charati JY. A comparison of microleakage in self-etch fissure sealants and conventional fissure sealants with total-etch or self-etch adhesive systems. Eur J Dent 2018; 12 (02) 242-246
  • 10 Bravo M, Montero J, Bravo JJ, Baca P, Llodra JC. Sealant and fluoride varnish in caries: a randomized trial. J Dent Res 2005; 84 (12) 1138-1143
  • 11 Pintanon P, Sattabanasuk V, Banomyong D. Effectiveness of caries infiltration and CPP-ACP containing paste on color change and surface hardness of artificial white spot enamel lesions. J Dent Assoc Thai 2016; 66 (02) 133-148
  • 12 Prasada KL, Penta PK, Ramya KM. Spectrophotometric evaluation of white spot lesion treatment using novel resin infiltration material (ICON®). J Conserv Dent 2018; 21 (05) 531-535
  • 13 Ciftci ZZ, Hanimeli S, Karayilmaz H, Gungor OE. The efficacy of resin infiltrate on the treatment of white spot lesions and developmental opacities. Niger J Clin Pract 2018; 21 (11) 1444-1449
  • 14 Paris S, Meyer-Lueckel H. Inhibition of caries progression by resin infiltration in situ. Caries Res 2010; 44 (01) 47-54
  • 15 Enan ET, Aref NS, Hammad SM. Resistance of resin-infiltrated enamel to surface changes in response to acidic challenge. J Esthet Restor Dent 2019; 31 (04) 353-358
  • 16 Kidd EA. Microleakage: a review. J Dent 1976; 4 (05) 199-206
  • 17 Martins GC, Sánchez-Ayala A, D'Alpino PH, Calixto AL, Gomes JC, Gomes OM. Interfacial integrity of bonded restorations with self-etching adhesives: water storage and thermo-mechanical cycling. Eur J Dent 2012; 6 (02) 169-177
  • 18 Klaisiri A, Janchum S, Wongsomtakoon K, Sirimanathon P, Krajangta N. Microleakage of resin infiltration in artificial white-spot lesions. J Oral Sci 2020; 62 (04) 427-429
  • 19 Ahlberg KM, Assavanop P, Tay WM. A comparison of the apical dye penetration patterns shown by methylene blue and India ink in root-filled teeth. Int Endod J 1995; 28 (01) 30-34
  • 20 ISO/TS 11405. Dentistry – Testing of Adhesion to Tooth Structure. Geneva, Switzerland. International Organization for Standardization; 2015. :7–9
  • 21 Germán-Cecilia C, Gallego Reyes SM, Pérez Silva A, Serna Muñoz C, Ortiz-Ruiz AJ. Microleakage of conventional light-cure resin-based fissure sealant and resin-modified glass ionomer sealant after application of a fluoride varnish on demineralized enamel. PLoS One 2018; 13 (12) e0208856
  • 22 Al Tuwirqi AA, Alshammari AM, Felemban OM, Ali Farsi NM. Comparison of penetration depth and microleakage of resin infiltrant and conventional sealant in pits and fissures of permanent teeth in vitro. J Contemp Dent Pract 2019; 20 (11) 1339-1344
  • 23 Li H, Burrow MF, Tyas MJ. The effect of thermocycling regimens on the nanoleakage of dentin bonding systems. Dent Mater 2002; 18 (03) 189-196
  • 24 Asaka Y, Amano S, Rikuta A. et al. Influence of thermal cycling on dentin bond strengths of single-step self-etch adhesive systems. Oper Dent 2007; 32 (01) 73-78
  • 25 Amaral FL, Colucci V, Palma-Dibb RG, Corona SA. Assessment of in vitro methods used to promote adhesive interface degradation: a critical review. J Esthet Restor Dent 2007; 19 (06) 340-353 , discussion 354
  • 26 Teixeira GS, Pereira GKR, Susin AH. Aging methods-an evaluation of their influence on bond strength. Eur J Dent 2021; 15 (03) 448-453
  • 27 Birkenfeld LH, Schulman A. Enhanced retention of glass-ionomer sealant by enamel etching: a microleakage and scanning electron microscopic study. Quintessence Int 1999; 30 (10) 712-718
  • 28 Singla A, Garg S, Jindal SK, Suma Sogi HP, Sharma D. In vitro evaluation of marginal leakage using invasive and noninvasive technique of light cure glass ionomer and flowable polyacid modified composite resin used as pit and fissure sealant. Indian J Dent Res 2011; 22 (02) 205-209
  • 29 Murayama R, Nagura Y, Yamauchi K. et al. Effect of a coating material containing surface reaction-type pre-reacted glass-ionomer filler on prevention of primary enamel demineralization detected by optical coherence tomography. J Oral Sci 2018; 60 (03) 367-373
  • 30 Spinola MDS, Moecke SE, Rossi NR, Nakatsuka T, Borges AB, Torres CRG. Efficacy of S-PRG filler containing varnishes on enamel demineralization prevention. Sci Rep 2020; 10 (01) 18992
  • 31 Rabiabasree R, Krishnakumar R, Prabhu AS, Naik NS, Shashibhushan KK, Janarthanan K. Inhibitory effect of a resin coat-containing prereacted glass fillers on the enamel demineralization of the primary teeth: an in vitro pilot study. J Indian Soc Pedod Prev Dent 2019; 37 (02) 146-150
  • 32 Espigares J, Hayashi J, Shimada Y, Tagami J, Sadr A. Enamel margins resealing by low-viscosity resin infiltration. Dent Mater J 2018; 37 (02) 350-357
  • 33 Meyer-Lueckel H, Paris S, Kielbassa AM. Surface layer erosion of natural caries lesions with phosphoric and hydrochloric acid gels in preparation for resin infiltration. Caries Res 2007; 41 (03) 223-230
  • 34 Lee J, Chen JW, Omar S, Kwon SR, Meharry M. Evaluation of stain penetration by beverages in demineralized enamel treated with resin infiltration. Oper Dent 2016; 41 (01) 93-102