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CC BY-NC-ND 4.0 · Rev Bras Ortop (Sao Paulo) 2023; 58(02): 271-278
DOI: 10.1055/s-0042-1750832
Artigo Original
Ombro e Cotovelo

Investigation the Effect of Human Recombinant Epidermal Growth Factor on Rotator Cuff Healing: An Experimental Model[*]

Artikel in mehreren Sprachen: português | English
Anil Gulcu 
1   Departamento de Ortopedia e Traumatologia, Faculdade de Medicina, Universidade Alanya Alaaddin Keykubat, Alanya/Antalya, Turquia
,
Egemen Turhan 
2   Departamento de Ortopedia e Traumatologia, Faculdade de Medicina, Universidade Hacettepe, Ankara, Turquia
,
Ahmet Aslan 
1   Departamento de Ortopedia e Traumatologia, Faculdade de Medicina, Universidade Alanya Alaaddin Keykubat, Alanya/Antalya, Turquia
,
Sevil Çaylı 
3   Departamento de Histologia e Embriologia, Faculdade de Medicina, Universidade Ankara Yıldırım Beyazıt, Ankara, Turquia
,
Ebru Alimogullari 
3   Departamento de Histologia e Embriologia, Faculdade de Medicina, Universidade Ankara Yıldırım Beyazıt, Ankara, Turquia
,
Gülben Akcan 
3   Departamento de Histologia e Embriologia, Faculdade de Medicina, Universidade Ankara Yıldırım Beyazıt, Ankara, Turquia
› Institutsangaben Financial Support There was no financial support from public, commercial, or non-profit sources.
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Abstract

Objective To investigate the effectiveness of human recombinant epidermal growth factor in the healing of rotator cuff tear in the rabbit shoulder.

Methods Rotator cuff tears (RCTs) were experimentally created on both shoulders of 20 New Zealand rabbits. The rabbits were divided into the following groups: RCT (sham group; n = 5), RCT + EGF (EGF group; n = 5), RCT + transosseous repair (repair group; n = 5), and RCT + EGF + transosseous repair (combined repair + EGF group; n = 5). All rabbits were then observed for 3 weeks, and biopsies were taken from the right shoulders in the third week. After three more weeks of observation, all rabbits were sacrificed, and a biopsy removed from their left shoulders. All biopsy material was stained with haematoxylin & eosin (H&E) and vascularity, cellularity, the proportion of fibers and the number of fibrocartilage cells were evaluated under light microscope.

Results The highest collagen amount and the most regular collagen sequence was detected in the combined repair + EGF group. The repair group and the EGF group showed higher fibroblastic activity and capillary formation when compared with the sham group, but the highest fibroblastic activity and capillary formation with highest vascularity was detected in the combined repair + EGF group (p < 0.001). EGF seems to improve wound healing in the repair of RCT. The EGF application alone, even without repair surgery, seems to be beneficial to RCT healing.

Conclusion In addition to rotator cuff tear repair, application of human recombinant epidermal growth factor has an effect on rotator cuff healing in rabbit shoulders.

Keywords

rotator cuff injuries - wound healing - epidermal growth factor

Authors' Contributions

Each author contributed individually and significantly to the development of this article:

A. G.: Conceptualization (lead), data curation, application of the animal experiment, and application of surgery in an animal experiment.

E. T., A. A.: Methodology, project administration, planning of the project.

S. Ç., E. A., G. A.: Histology and embryology.


* Study developed at the Faculty of Medicine, Alanya Alaaddin Keykubat University, Alanya/Antalya, Turkey.




Publikationsverlauf

Eingereicht: 13. November 2021

Angenommen: 28. April 2022

Artikel online veröffentlicht:
06. Juli 2022

© 2022. Sociedade Brasileira de Ortopedia e Traumatologia. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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