Zeitschrift für Phytotherapie 2022; 43(S 01): S36
DOI: 10.1055/s-0042-1749263
Abstracts Poster | Phytotherapie 2022 – innovativ

Effect of Petasites hybridus extract Ze 339 on organic cation transporter 3 – consequences for intestinal handling of histamine

K Brecht Brüngger
1   Biopharmacy, Department Pharmaceutical Sciences, University of Basel, 4001 Basel, Switzerland
,
L Mettler
1   Biopharmacy, Department Pharmaceutical Sciences, University of Basel, 4001 Basel, Switzerland
,
V Butterweck
2   Max Zeller & Söhne AG, Research and Development, 8590 Romanshorn, Switzerland
,
H Meyer zu Schwabedissen
1   Biopharmacy, Department Pharmaceutical Sciences, University of Basel, 4001 Basel, Switzerland
› Author Affiliations
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Introduction Histamine intolerance is a common diagnosis in Western population with an estimated prevalence of 1% in a population [1]. The uptake of food-derived histamine is assumed to be modulated by specific mechanisms in the intestine. Here, diamine oxidase (DAO) and histamine-N-methyltransferase (HNMT) metabolize histamine, while transport proteins are believed to contribute to the transcellular histamine flux. Anecdotal evidence from patients with HIT suggests an improvement of symptoms related to histamine intolerance after intake of Ze 339 [2].

Aim Here, we investigate the influence of Petasites hybridus CO2-leaf extract Ze 339 on intestinal mechanisms involved in histamine handling in vitro.

Methods We used differentiated Caco-2 cells investigating the effect of Ze 339 on DAO, HNMT and organic cation transporter 3 (OCT3) mRNA and protein expression [3]. We further tested the effect of Ze 339 on DAO release from Caco-2 cells polarized on permeable supports and DAO enzyme activity. We applied Caco-2 Transwell®​ transport studies to assess the effect of Ze 339 on the transcellular histamine flux. Findings on changes in flux were supplemented with transport studies using MDCKII-OCT3 cells.

Results Ze 339 reduced mRNA levels of HNMT and DAO, but there was no influence on their protein levels. We found that Ze 339 neither affected basal DAO release, nor DAO activity. In our transport studies we observed a significant increase in the basal to apical flux in presence of high concentrations of Ze 339 at the early phase of the experiment. Using stable MDCKII-OCT3 cells, we found that Ze 339 dose-dependently inhibited OCT3-mediated histamine uptake.

Conclusion We report an effect of Ze 339 on the transcellular transport of histamine in vitro, where inhibition of the luminal uptake transporter OCT3 may contribute.



Publication History

Article published online:
13 June 2022

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