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CC BY 4.0 · Eur J Dent 2022; 16(02): 367-372
DOI: 10.1055/s-0041-1735907
Original Article

Association of HPV and EBV in Oral Verrucous Squamous Cell Carcinoma and Oral Verrucous Hyperplasia

Eakapong Tamboon
1   Department of Oral Biology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
,
Phetmany Sihavong
2   Dental Basic Science Department, Faculty of Dentistry, University of Health Science, Lao P.D.R, Thailand
3   Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
,
Nakarin Kitkumthorn
1   Department of Oral Biology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
,
Dusit Bumalee
3   Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
,
Tawepong Arayapisit
4   Department of Anatomy, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
,
Puangwan Lapthanasupkul
3   Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
› Author Affiliations Funding This research was partially supported by International Dental Collaboration of The Mekong River Region (IDCMR) scholarship, Faculty of Dentistry, Mahidol University.
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Abstract

Objective Oral verrucous squamous cell carcinoma or oral verrucous carcinoma (OVC) is a rare verrucous variant of oral squamous cell carcinoma (OSCC), which accounts for 2 to 12% of all oral carcinomas. Oral verrucous hyperplasia (OVH) is clinically similar to OVC and has been proposed to be a precursor lesion of OVC. Etiopathogenesis of both lesions is still inconspicuous. Oncogenic viruses such as human papillomavirus (HPV) and Epstein–Barr virus (EBV) have been reported to be associated with some cases of OSCC, and we hypothesized that it may act as a causative agent of these verrucous lesions. This study aimed to investigate frequency of HPV and EBV infections in OVC and OVH.

Material and Methods Using polymerase chain reaction (PCR), a total of 35 formalin-fixed paraffin-embedded (FFPE) tissue samples, including 27 OVC samples and 8 OVH samples, were investigated for HPV and EBV infection. HeLa and B95-8 cell lines were used as positive controls of HPV and EBV PCR, respectively.

Results All OVC and OVH samples show a positivity to GAPDH, whereas neither HPV nor EBV PCR products was detected in both OVC and OVH samples.

Conclusions In summary, our study demonstrated that HPV and EBV are not involved in pathogenesis of OVC and OVH. Other etiologic factors contributing to OVC and OVH need to be further clarified.

Keywords

oral verrucous squamous cell carcinoma - oral verrucous carcinoma - oral verrucous hyperplasia - HPV - EBV


Publication History

Article published online:
02 December 2021

© 2021. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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  • References

  • 1 Walvekar RR, Chaukar DA, Deshpande MS. et al. Verrucous carcinoma of the oral cavity: A clinical and pathological study of 101 cases. Oral Oncol 2009; 45 (01) 47-51
    CrossrefPubMedGoogle Scholar
  • 2 Rekha KP, Angadi PV. Verrucous carcinoma of the oral cavity: a clinico-pathologic appraisal of 133 cases in Indians. Oral Maxillofac Surg 2010; 14 (04) 211-218
    CrossrefPubMedGoogle Scholar
  • 3 Peng Q, Wang Y, Quan H, Li Y, Tang Z. Oral verrucous carcinoma: from multifactorial etiology to diverse treatment regimens (Review). Int J Oncol 2016; 49 (01) 59-73
    CrossrefPubMedGoogle Scholar
  • 4 Noble-Topham SE, Fliss DM, Hartwick RW. et al. Detection and typing of human papillomavirus in verrucous carcinoma of the oral cavity using the polymerase chain reaction. Arch Otolaryngol Head Neck Surg 1993; 119 (12) 1299-1304
    CrossrefPubMedGoogle Scholar
  • 5 Fujita S, Senba M, Kumatori A, Hayashi T, Ikeda T, Toriyama K. Human papillomavirus infection in oral verrucous carcinoma: genotyping analysis and inverse correlation with p53 expression. Pathobiology 2008; 75 (04) 257-264
    CrossrefPubMedGoogle Scholar
  • 6 de Spíndula-Filho JV, da Cruz AD, Oton-Leite AF. et al. Oral squamous cell carcinoma versus oral verrucous carcinoma: an approach to cellular proliferation and negative relation to human papillomavirus (HPV). Tumour Biol 2011; 32 (02) 409-416
    CrossrefPubMedGoogle Scholar
  • 7 Lin HP, Wang YP, Chiang CP. Expression of p53, MDM2, p21, heat shock protein 70, and HPV 16/18 E6 proteins in oral verrucous carcinoma and oral verrucous hyperplasia. Head Neck 2011; 33 (03) 334-340
    CrossrefPubMedGoogle Scholar
  • 8 Shannon-Lowe C, Rickinson A. The global landscape of EBV-associated tumors. Front Oncol 2019; 9: 713
    CrossrefPubMedGoogle Scholar
  • 9 Wilson JB, Weinberg W, Johnson R, Yuspa S, Levine AJ. Expression of the BNLF-1 oncogene of Epstein-Barr virus in the skin of transgenic mice induces hyperplasia and aberrant expression of keratin 6. Cell 1990; 61 (07) 1315-1327
    CrossrefPubMedGoogle Scholar
  • 10 Jang HS, Cho JO, Yoon CY, Kim HJ, Park JC. Demonstration of Epstein-Barr virus in odontogenic and nonodontogenic tumors by the polymerase chain reaction (PCR). J Oral Pathol Med 2001; 30 (10) 603-610
    CrossrefPubMedGoogle Scholar
  • 11 Bagan JV, Jiménez Y, Murillo J. et al. Epstein-Barr virus in oral proliferative verrucous leukoplakia and squamous cell carcinoma: A preliminary study. Med Oral Patol Oral Cir Bucal 2008; 13 (02) E110-E113
    PubMedGoogle Scholar
  • 12 Zhu LK, Ding YW, Liu W, Zhou YM, Shi LJ, Zhou ZT. A clinicopathological study on verrucous hyperplasia and verrucous carcinoma of the oral mucosa. J Oral Pathol Med 2012; 41 (02) 131-135
    CrossrefPubMedGoogle Scholar
  • 13 Alkan A, Bulut E, Gunhan O, Ozden B. Oral verrucous carcinoma: a study of 12 cases. Eur J Dent 2010; 4 (02) 202-207
    Article in Thieme ConnectPubMedGoogle Scholar
  • 14 Kasperbauer JL, O'Halloran GL, Espy MJ, Smith TF, Lewis JE. Polymerase chain reaction (PCR) identification of human papillomavirus (HPV) DNA in verrucous carcinoma of the larynx. Laryngoscope 1993; 103 (4 Pt 1): 416-420
    CrossrefPubMedGoogle Scholar
  • 15 Shroyer KR, Greer Jr RO. Detection of human papillomavirus DNA by in situ DNA hybridization and polymerase chain reaction in premalignant and malignant oral lesions. Oral Surg Oral Med Oral Pathol 1991; 71 (06) 708-713
    CrossrefPubMedGoogle Scholar
  • 16 del Pino M, Bleeker MC, Quint WG, Snijders PJ, Meijer CJ, Steenbergen RD. Comprehensive analysis of human papillomavirus prevalence and the potential role of low-risk types in verrucous carcinoma. Mod Pathol 2012; 25 (10) 1354-1363
    CrossrefPubMedGoogle Scholar
  • 17 Mitsuishi T, Ohara K, Kawashima M, Kobayashi S, Kawana S. Prevalence of human papillomavirus DNA sequences in verrucous carcinoma of the lip: genomic and therapeutic approaches. Cancer Lett 2005; 222 (02) 139-143
    CrossrefPubMedGoogle Scholar
  • 18 Shroyer KR, Greer RO, Fankhouser CA, McGuirt WF, Marshall R. Detection of human papillomavirus DNA in oral verrucous carcinoma by polymerase chain reaction. Mod Pathol 1993; 6 (06) 669-672
    PubMedGoogle Scholar
  • 19 Saghravanian N, Ghazvini K, Babakoohi S, Firooz A, Mohtasham N. Low prevalence of high risk genotypes of human papilloma virus in normal oral mucosa, oral leukoplakia and verrucous carcinoma. Acta Odontol Scand 2011; 69 (06) 406-409
    CrossrefPubMedGoogle Scholar
  • 20 Stokes A, Guerra E, Bible J. et al. Human papillomavirus detection in dysplastic and malignant oral verrucous lesions. J Clin Pathol 2012; 65 (03) 283-286
    CrossrefPubMedGoogle Scholar
  • 21 Sritippho T, Pongsiriwet S, Lertprasertsuke N, Buddhachat K, Sastraruji T, Iamaroon A. p16 - A possible surrogate marker for high-risk human papillomaviruses in oral cancer?. Asian Pac J Cancer Prev 2016; 17 (08) 4049-4057
    PubMedGoogle Scholar
  • 22 Gualco M, Bonin S, Foglia G. et al. Morphologic and biologic studies on ten cases of verrucous carcinoma of the vulva supporting the theory of a discrete clinico-pathologic entity. Int J Gynecol Cancer 2003; 13 (03) 317-324
    CrossrefPubMedGoogle Scholar
  • 23 Masih AS, Stoler MH, Farrow GM, Wooldridge TN, Johansson SL. Penile verrucous carcinoma: a clinicopathologic, human papillomavirus typing and flow cytometric analysis. Mod Pathol 1992; 5 (01) 48-55
    PubMedGoogle Scholar
  • 24 Nascimento AF, Granter SR, Cviko A, Yuan L, Hecht JL, Crum CP. Vulvar acanthosis with altered differentiation: a precursor to verrucous carcinoma?. Am J Surg Pathol 2004; 28 (05) 638-643
    CrossrefPubMedGoogle Scholar
  • 25 Chuanyu S, Ke X, Jie Z, Guowei X, Zujun F, Qiang D. Surgical treatment for 11 cases of penile verrucous carcinoma. Ann Dermatol 2011; 23 (Suppl. 03) S346-S349
    CrossrefPubMedGoogle Scholar
  • 26 Horiuchi K, Mishima K, Ichijima K, Sugimura M, Ishida T, Kirita T. Epstein-Barr virus in the proliferative diseases of squamous epithelium in the oral cavity. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1995; 79 (01) 57-63
    CrossrefPubMedGoogle Scholar
  • 27 Shimakage M, Horii K, Tempaku A, Kakudo K, Shirasaka T, Sasagawa T. Association of Epstein-Barr virus with oral cancers. Hum Pathol 2002; 33 (06) 608-614
    CrossrefPubMedGoogle Scholar
  • 28 Higa M, Kinjo T, Kamiyama K, Iwamasa T, Hamada T, Iyama K. Epstein-Barr virus (EBV) subtype in EBV related oral squamous cell carcinoma in Okinawa, a subtropical island in southern Japan, compared with Kitakyushu and Kumamoto in mainland Japan. J Clin Pathol 2002; 55 (06) 414-423
    CrossrefPubMedGoogle Scholar
  • 29 Iamaroon A, Khemaleelakul U, Pongsiriwet S, Pintong J. Co-expression of p53 and Ki67 and lack of EBV expression in oral squamous cell carcinoma. J Oral Pathol Med 2004; 33 (01) 30-36
    CrossrefPubMedGoogle Scholar
  • 30 Hazarey VK, Ganvir SM, Bodhade AS. Verrucous hyperplasia: a clinico-pathological study. J Oral Maxillofac Pathol 2011; 15 (02) 187-191
    CrossrefPubMedGoogle Scholar
  • 31 Srinivasan M, Sedmak D, Jewell S. Effect of fixatives and tissue processing on the content and integrity of nucleic acids. Am J Pathol 2002; 161 (06) 1961-1971
    CrossrefPubMedGoogle Scholar