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CC BY-NC-ND 4.0 · Eur J Dent 2021; 15(04): 653-659
DOI: 10.1055/s-0041-1727554
Original Article

The Effects of Rice Husk Liquid Smoke in Porphyromonas gingivalis-Induced Periodontitis

Theresia Indah Budhy
1   Department of Oral Pathology and Maxillofacial, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Ira Arundina
2   Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Meircurius Dwi Condro Surboyo 
3   Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Anisa Nur Halimah
4   Master of Dental Science Program, Faculty of Dental Medicine. Universitas Airlangga, Surabaya, Indonesia
› Institutsangaben Funding This study was funded by Ministry of Higher Education, Republic of Indonesia 2020 in the schema PENELITIAN DASAR UNGGULAN PERGURUAN TINGGI (PDUPT) (funding number 630/UN3.14/PT/2020).
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Abstract

Objectives The purpose of this study was to analyze the effects of rice husk liquid smoke in Porphyromonas gingivalis-induced periodontitis in the inflammatory and proliferation marker such as nuclear factor kappa β (NF-kB), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), transforming growth factor-β (TGF-β), fibroblast growth factor 2 (FGF2), collagen type 1 (COL-1) expression, and the number of macrophages, lymphocytes, and fibroblasts.

Materials and Methods Rice husk liquid smoke is obtained by the pyrolysis process. Porphyromonas gingivalis-induced periodontitis in 20 μL phosphate-buffered saline containing 1 × 109 CFU was injected into the lower anterior gingival sulcus of Wistar rats. The periodontitis was then treated with 20 μL/20 g body weight of rice husk liquid smoke once a day for 2 and 7 days, respectively. After treatment, the bone and lower anterior gingival sulcus were analyzed with immunohistochemistry and hematoxylin–eosin staining.

Results The treatment of periodontitis with rice husk liquid smoke showed a lower NF-kB, TNF-α, and IL-6 expression and a higher TGF-β, FGF2, and COL-1 expression than the control after treatment for 2 and 7 days (p < 0.05), respectively. The number of macrophages and fibroblasts was also higher when compared with the control group (p < 0.05), but the number of lymphocytes was lower than the control (p < 0.05).

Conclusion Rice husk liquid smoke showed its effects on Porphyromonas gingivalis-induced periodontitis with a decrease in inflammatory markers and an increase in proliferation markers. The development of a rice husk liquid smoke periodontitis treatment is promising.

Keywords

Porphyromonas gingivalis - periodontitis - liquid smoke - rice husk


Publikationsverlauf

Artikel online veröffentlicht:
26. Mai 2021

© 2021. European Journal of Dentistry. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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  • References

  • 1 Lingbeck JM, Cordero P, O’Bryan CA, Johnson MG, Ricke SC, Crandall PG. Functionality of liquid smoke as an all-natural antimicrobial in food preservation. Meat Sci 2014; 97 (02) 197-206
    CrossrefPubMedSuche in Google Scholar
  • 2 Nithin CT, Joshy CG, Chatterjee NS. et al Liquid smoking - a safe and convenient alternative for traditional fish smoked products. Food Control 2020; 113 (July) 107186
    CrossrefSuche in Google Scholar
  • 3 Saloko S, Darmadji P, Setiaji B, Pranoto Y. Antioxidative and antimicrobial activities of liquid smoke nanocapsules using chitosan and maltodextrin and its application on tuna fish preservation. Food Biosci 2014; 7: 71-79
    CrossrefSuche in Google Scholar
  • 4 Yusnaini S, Suryanto E, Armunanto R. Physical, chemical and sensory properties of kenari (Canariun indicum L.) shell liquid smoke-immersed-beef on different level of dilution. J Indones Trop Anim Agric 2012; 37 (01) 27-33
    CrossrefSuche in Google Scholar
  • 5 Sokamte Tegang A, Mbougueng PD, Sachindra NM, Douanla Nodem NF, Tatsadjieu Ngoune L. Characterization of volatile compounds of liquid smoke flavourings from some tropical hardwoods. Sci African 2020; 8: e00443
    CrossrefSuche in Google Scholar
  • 6 Soares JM, da Silva PF, Puton BMS. et al Antimicrobial and antioxidant activity of liquid smoke and its potential application to bacon. Innov Food Sci Emerg Technol 2016; 38: 189-197
    CrossrefSuche in Google Scholar
  • 7 C T N, Sekhar ChatterjeeN. C G J. et al Source-dependent compositional changes in coconut flavoured liquid smoke and its application in traditional Indian smoked fishery products. Food Addit Contam Part A Chem Anal Control Expo Risk Assess 2020; 37 (10) 1610-1620
    CrossrefPubMedSuche in Google Scholar
  • 8 Faisal M, Gani A, Mulana F. Preliminary assessment of the utilization of durian peel liquid smoke as a natural preservative for mackerel. F1000 Res 2019; 8: 240
    CrossrefPubMedSuche in Google Scholar
  • 9 Kailaku S, Syakir M, Mulyawanti I, Syah A. Antimicrobial activity of coconut shell liquid smoke. IOP Conf Ser. Mater Sci Eng 2017; 206: 12050
    Suche in Google Scholar
  • 10 Surboyo MDC, Arundina I, Rahayu RP, Mansur D, Bramantoro T. Potential of distilled liquid smoke derived from coconut (Cocos nucifera L) shell for traumatic ulcer healing in diabetic rats. Eur J Dent 2019; 13 (02) 271-279
    Thieme ConnectPubMedSuche in Google Scholar
  • 11 Arundina I, Diyatri I, Surboyo MDC. The component analysis of liquid smoke from rice hulls and its toxicity test on baby hamster kidney cells. J Pharm Phyther Res. 2021; 9 (01) 78-87
    Suche in Google Scholar
  • 12 Arundina I, Tantiana T, Diyatri I, Surboyo MDC, Adityasari R. Acute toxicity of liquid smoke of rice hull (Oryza sativa) on mice (Mus musculus). J. Int Dent Med Res. 2020; 13 (01) 91-96
    Suche in Google Scholar
  • 13 Tarawan VM, Mantilidewi KI, Dhini IM, Radhiyanti PT, Sutedja E. Coconut shell liquid smoke promotes burn wound healing. J Evid Based Complementary Altern Med 2017; 22 (03) 436-440
    CrossrefPubMedSuche in Google Scholar
  • 14 Arundina I, Diyatri I, Kusumaningsih T, Surboyo MDC, Monica E, Afanda NM. The role of rice hull liquid smoke in the traumatic ulcer healing. Eur J Dent 2020; 15 (01) 33-38
    PubMedSuche in Google Scholar
  • 15 Kim SP, Yang JY, Kang MY, Park JC, Nam SH, Friedman M. Composition of liquid rice hull smoke and anti-inflammatory effects in mice. J Agric Food Chem 2011; 59 (09) 4570-4581
    CrossrefPubMedSuche in Google Scholar
  • 16 Yang JY, Kang MY, Nam SH, Friedman M. Antidiabetic effects of rice hull smoke extract in alloxan-induced diabetic mice. J Agric Food Chem 2012; 60 (01) 87-94
    CrossrefPubMedSuche in Google Scholar
  • 17 Rafiei M, Kiani F, Sayehmiri F, Sayehmiri K, Sheikhi A, Zamanian Azodi M. Study of Porphyromonas gingivalis in periodontal diseases: a systematic review and meta-analysis. Med J Islam Repub Iran 2017; 31: 62
    CrossrefPubMedSuche in Google Scholar
  • 18 Elkaim R, Bugueno-Valdebenito IM, Benkirane-Jessel N, Tenenbaum H. Porphyromonas gingivalis and its lipopolysaccharide differently modulate epidermal growth factor-dependent signaling in human gingival epithelial cells. J Oral Microbiol 2017; 9 (01) 1334503
    CrossrefPubMedSuche in Google Scholar
  • 19 Groeger S, Jarzina F, Domann E, Meyle J. Porphyromonas gingivalis activates NFκB and MAPK pathways in human oral epithelial cells. BMC Immunol 2017; 18 (01) 1
    CrossrefPubMedSuche in Google Scholar
  • 20 Watanabe N, Yokoe S, Ogata Y, Sato S, Imai K. Exposure to Porphyromonas gingivalis induces production of proinflammatory cytokine via TLR2 from human respiratory epithelial cells. J Clin Med 2020; 9 (11) 3433
    CrossrefPubMedSuche in Google Scholar
  • 21 Son G-Y, Bak E-J, Kim J-H. et al Endothelin regulates porphyromonas gingivalis-induced production of inflammatory cytokines. PLoS One 2016; 11 (12) e0167713
    CrossrefPubMedSuche in Google Scholar
  • 22 Wang H, Zhou H, Duan X. et al Porphyromonas gingivalis- induced reactive oxygen species activate JAK2 and regulate production of inflammatory cytokines through c-Jun. Infect Immun 2014; 82 (10) 4118-4126
    CrossrefPubMedSuche in Google Scholar
  • 23 Arundina IRA, Diyatri I, Surboyo MDC, Halimah AN, Chusnurrafi FI. The antibacterial effect of liquid smoke rice hull on porphyromonas gingivalis and its proliferative effects on osteoblast as periodontitis remedies: an in vitro study. Int J Pharm Res. 2020; 12 (03) 3466-3471
    Suche in Google Scholar
  • 24 Ridwan RD, Tantiana T, Setijanto D, Kusuma AK, Putranto AF. The ability of electrolyzed reduced water to act as an antioxidant and anti-inflammatory agent in chronic periodontitis Wistar rats (Rattus novergicus).. Kafkas Univ Vet Fak Derg 2019; 25 (04) 539-544
    Suche in Google Scholar
  • 25 Santos BFE, Souza EQM, Brigagão MRPL, Lima DC, Fernandes LA. Local application of statins in the treatment of experimental periodontal disease in rats. J Appl Oral Sci 2017; 25 (02) 168-176
    CrossrefPubMedSuche in Google Scholar
  • 26 Jia L, Han N, Du J, Guo L, Luo Z, Liu Y. Pathogenesis of important virulence factors ofPorphyromonas gingivalis via Toll-like receptors. Front Cell Infect Microbiol 2019; 9: 262
    CrossrefPubMedSuche in Google Scholar
  • 27 Zhao DR, Jiang YS, Sun JY, Li HH, Luo XL, Zhao MM. Anti-inflammatory mechanism involved in 4-ethylguaiacol- mediated inhibition of LPS-induced inflammation in THP-1 cells. J Agric Food Chem 2019; 67 (04) 1230-1243
    CrossrefPubMedSuche in Google Scholar
  • 28 Zhao DR, Jiang YS, Sun JY, Li HH, Sun XT, Zhao MM. Amelioration of 4-methylguaiacol on LPS-induced inflammation in THP-1 cells through NF-κB/IκBα/AP-1 and Nrf2/HO-1 signaling pathway. J Funct Foods 2019; 55 (January) 95-103
    CrossrefSuche in Google Scholar
  • 29 Zhao D, Sun J, Sun B. et al Intracellular antioxidant effect of vanillin, 4-methylguaiacol and 4-ethylguaiacol: three components in Chinese Baijiu. RSC Advances 2017; 7 (73) 46395-46405
    CrossrefSuche in Google Scholar
  • 30 Ayuningtyas NF, Dwi M, Surboyo C, Ernawati DS, Parmadiati AE. The role of liquid smoke coconut shell in the proliferation phase of an oral traumatic ulcer. J Pharm Pharmacogn Res. 2020; 8 (06) 549-557
    Suche in Google Scholar
  • 31 Surboyo MDC, Mahdani FY, Ernawati DS, Sarasati A, Rezkita F. The macrophage responses during diabetic oral ulcer healing by liquid coconut shell smoke: an immunohistochemical analysis. Eur J Dent 2020; 14 (03) 410-414
    Thieme ConnectPubMedSuche in Google Scholar
  • 32 Ernawati DS, Surboyo MDC, Ayuningtyas NF, Nagoro AAB. Role of inflammatory cell responses in stimulating fibroblasts in diabetic oral ulcer after treatment with liquid smoke of coconut endocarp: a histological assessment. Eur J Dent 2020; 15 (01) 71-76
    PubMedSuche in Google Scholar
  • 33 Aldulaimi OA. General overview of phenolics from plant to laboratory, good antibacterials or not. Pharmacogn Rev 2017; 11 (22) 123-127
    CrossrefPubMedSuche in Google Scholar
  • 34 Papuc C, Goran GV, Predescu CN, Nicorescu V, Stefan G. Plant polyphenols as antioxidant and antibacterial agents for shelf- life extension of meat and meat products: classification, structures, sources, and action mechanisms. Compr Rev Food Sci Food Saf 2017; 16 (06) 1243-1268
    CrossrefPubMedSuche in Google Scholar