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CC BY-NC-ND 4.0 · Eur J Dent 2021; 15(03): 533-538
DOI: 10.1055/s-0041-1723068
Original Article

Final Endodontic Irrigation with 2% Peracetic Acid: Antimicrobial Activity and Cytotoxicity

Daniel de Oliveira Brandão-Neto
1   Postgraduate Program in Dentistry, Health Institute of Nova Friburgo, Fluminense Federal University, Nova Friburgo, Rio de Janeiro, Brazil.
,
João Victor Zebende Mello
2   School of Dentistry, Health Institute of Nova Friburgo, Fluminense Federal University, Nova Friburgo, Rio de Janeiro, Brazil.
,
Marilia Fagury Videira Marceliano-Alves
3   Post Graduation Program in Dentistry and Research, Iguaçu University, Nova Iguaçu, Rio de Janeiro, Brazil.
,
Thais Machado de Carvalho Coutinho
3   Post Graduation Program in Dentistry and Research, Iguaçu University, Nova Iguaçu, Rio de Janeiro, Brazil.
,
Eduardo Fagury Videira Marceliano
4   Dental Clinic Department, Brazilian Army General Hospital of Belem, Belem, Brazil.
,
Michelle Paiva Weydt Galhardi
5   Department of Dental Materials, Sao Jose University, Rio de Janeiro, Brazil.
,
Vívian Souza Tavares
2   School of Dentistry, Health Institute of Nova Friburgo, Fluminense Federal University, Nova Friburgo, Rio de Janeiro, Brazil.
,
Aline Peçanha Muzy Dias
2   School of Dentistry, Health Institute of Nova Friburgo, Fluminense Federal University, Nova Friburgo, Rio de Janeiro, Brazil.
,
Renata Ximenes Lins
1   Postgraduate Program in Dentistry, Health Institute of Nova Friburgo, Fluminense Federal University, Nova Friburgo, Rio de Janeiro, Brazil.
› Author Affiliations Funding This study was supported by grants from Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ), a Brazilian governmental institution.
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Abstract

Objective The aim of present study was to assess the cytotoxicity and antimicrobial efficacy of 2% peracetic acid (PAA) compared with 5.25% sodium hypochlorite (NaOCl) and 2% chlorhexidine (CHX).

Material and Methods For the cytotoxicity test, 100 µl of the tested solutions were added in 12 wells with ECV 304 endothelial cells in each group: NaOCl, CHX, and PAA, in addition to the control group. Each solution was evaluated after 24 hours of contact in four dilutions: 0.2, 0.1, 0.05 and 0.025 through mitochondrial function using MTT colorimetric assay. In the antimicrobial evaluation, 40 dentin blocks 5 mm in length and 0.2 g in weight were incubated with 400 µl of Enterococcus faecalis suspension for 21 days at 37°C. The contaminated samples were divided into three experimental groups within 5 minutes of contact: NaOCl group, CHX group, PAA group, as well as the positive control group. The specimens received treatment and were transferred to a tube with saline for serial dilution of the solution and seeding for isolation and colony forming unit (CFU) count.

Statistical Analysis The results obtained were expressed as mean (A570 nm) ± standard deviation (SD) and in a multiple linear regression model and multiple comparisons conducted.

Results The antimicrobial evaluation revealed that the NaOCl and CHX groups showed a statistically significant difference compared with the control group (p < 0.001), while the PAA reduced only the CFU growth. It can be concluded that, among the agents tested, PAA expressed greater cell viability, followed by CHX and NaOCl. However, it did not show greater antimicrobial activity in vitro in the mature biofilm of Enterococcus faecalis.

Keywords

chlorhexidine - disinfection - enterococcus faecalis - peracetic acid - sodium hypochlorite


Publication History

Article published online:
03 February 2021

© 2021. European Journal of Dentistry. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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