CC BY-NC-ND 4.0 · Eur J Dent 2021; 15(03): 515-522
DOI: 10.1055/s-0040-1722484
Original Article

In Vitro Enamel Remineralization Efficacy of Calcium Silicate-Sodium Phosphate-Fluoride Salts versus NovaMin Bioactive Glass, Following Tooth Whitening

Hatem M. El-Damanhoury
1   Department of Preventive and Restorative Dentistry, College of Dental Medicine, University of Sharjah, Sharjah, United Arab Emirates
,
Nesrine A. Elsahn
2   Department of Clinical Sciences, College of Dentistry, Ajman University, Ajman, United Arab Emirates
3   Department of Operative Dentistry, Faculty of Dentistry, Cairo University, Cairo, Egypt
,
Soumya Sheela
4   Dental Biomaterials Research Group, Sharjah Institute for Medical Research, University of Sharjah, Sharjah, United Arab Emirates
,
Talal Bastaty
5   College of Dental Medicine, University of Sharjah, Sharjah, United Arab Emirates
› Author Affiliations
Funding None.

Abstract

Objectives This study aimed to evaluate the effect of in-office bleaching on the enamel surface and the efficacy of calcium silicate-sodium phosphate-fluoride salt (CS) and NovaMin bioactive glass (NM) dentifrice in remineralizing bleached enamel.

Materials and Methods Forty extracted premolars were sectioned mesio-distally, and the facial and lingual enamel were flattened and polished. The samples were equally divided into nonbleached and bleached with 38% hydrogen peroxide (HP). Each group was further divided according to the remineralization protocol (n = 10); no remineralization treatment (nontreated), CS, or NM, applied for 3 minutes two times/day for 7 days, or CS combined with NR-5 boosting serum (CS+NR-5) applied for 3 minutes once/day for 3 days. The average Knoop hardness number (KHN) and surface roughness (utilizing atomic force microscopy) were measured. Surface topography/elemental analysis was analyzed by using scanning electron microscopy/energy dispersive X-ray analysis. All the tests were performed at baseline, after bleaching, and following each remineralization protocol. Data were statistically analyzed by two-way analysis of variance and Bonferroni post hoc multiple comparison tests (α = 0.05).

Results HP significantly reduced KHN and increased roughness (p < 0.05). All remineralization materials increased the hardness and reduced the surface roughness after bleaching except NM, which demonstrated significantly increased roughness (p < 0.05). Ca/P ratio decreased after bleaching (p < 0.05), and following treatment, CS and CS+NR-5 exhibited higher remineralization capacity in comparison to NM (p < 0.05).

Conclusion Although none of the material tested was able to reverse the negative effect of high-concentration in-office HP on enamel completely, the remineralization efficacy of CS and CS+NR-5 was superior to that of NM.



Publication History

Article published online:
23 February 2021

© 2021. European Journal of Dentistry. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

Thieme Medical and Scientific Publishers Pvt. Ltd.
A-12, 2nd Floor, Sector 2, Noida-201301 UP, India

 
  • References

  • 1 Rastelli ANS, Nicolodelli G, Romano RA. et al. After bleaching enamel remineralization using a bioactive glass-ceramic (BioSilicate). Biomed Glas 2016; 2 (01) 1-9
  • 2 Tredwin CJ, Naik S, Lewis NJ, Scully C. Hydrogen peroxide tooth-whitening (bleaching) products: review of adverse effects and safety issues. Br Dent J 2006; 200 (07) 371-376
  • 3 Markowitz K. Pretty painful: why does tooth bleaching hurt?. Med Hypotheses 2010; 74 (05) 835-840
  • 4 Rodrigues JA, Oliveira GPF, Amaral CM. Effect of thickener agents on dental enamel microhardness submitted to at-home bleaching. Braz Oral Res 2007; 21 (02) 170-175
  • 5 Haywood VB, Leech T, Heymann HO, Crumpler D, Bruggers K. Nightguard vital bleaching: effects on enamel surface texture and diffusion. Quintessence Int 1990; 21 (10) 801-804
  • 6 Miranda CB, Pagani C, Benetti AR, Matuda F da S. Evaluation of the bleached human enamel by scanning electron microscopy. J Appl Oral Sci 2005; 13 (02) 204-211
  • 7 Bitter NC. A scanning electron microscopy study of the effect of bleaching agents on enamel: a preliminary report. J Prosthet Dent 1992; 67 (06) 852-855
  • 8 Elfallah HM, Swain MV. A review of the effect of vital teeth bleaching on the mechanical properties of tooth enamel. N Z Dent J 2013; 109 (03) 87-96
  • 9 Ushigome T, Takemoto S, Hattori M, Yoshinari M, Kawada E, Oda Y. Influence of peroxide treatment on bovine enamel surface–cross-sectional analysis. Dent Mater J 2009; 28 (03) 315-323
  • 10 Dominguez JA, Bittencourt B, Michel M, Sabino N, Gomes JC, Gomes OMM. Ultrastructural evaluation of enamel after dental bleaching associated with fluoride. Microsc Res Tech 2012; 75 (08) 1093-1098
  • 11 Attin T, Kielbassa AM, Schwanenberg M, Hellwig E. Effect of fluoride treatment on remineralization of bleached enamel. J Oral Rehabil 1997; 24 (04) 282-286
  • 12 Coceska E, Gjorgievska E, Coleman NJ. et al. Enamel alteration following tooth bleaching and remineralization. J Microsc 2016; 262 (03) 232-244
  • 13 Sun Y, Li X, Deng Y. et al. Mode of action studies on the formation of enamel minerals from a novel toothpaste containing calcium silicate and sodium phosphate salts. J Dent 2014; 42 (Suppl. 01) S30-S38
  • 14 Gjorgievska E, Nicholson JW. Prevention of enamel demineralization after tooth bleaching by bioactive glass incorporated into toothpaste. Aust Dent J 2011; 56 (02) 193-200
  • 15 Andersson OH, Kangasniemi I. Calcium phosphate formation at the surface of bioactive glass in vitro. J Biomed Mater Res 1991; 25 (08) 1019-1030
  • 16 Zhong JP, Greenspan DC, Feng JW. A microstructural examination of apatite induced by Bioglass in vitro. J Mater Sci Mater Med 2002; 13 (03) 321-326
  • 17 Holland RI. Corrosion testing by potentiodynamic polarization in various electrolytes. Dent Mater 1992; 8 (04) 241-245
  • 18 Cadenaro M, Navarra CO, Mazzoni A. et al. An in vivo study of the effect of a 38 percent hydrogen peroxide in-office whitening agent on enamel. J Am Dent Assoc 2010; 141 (04) 449-454
  • 19 Hosoya N, Honda K, Iino F, Arai T. Changes in enamel surface roughness and adhesion of Streptococcus mutans to enamel after vital bleaching. J Dent 2003; 31 (08) 543-548
  • 20 Basting RT, Rodrigues Jr AL, Serra MC. The effects of seven carbamide peroxide bleaching agents on enamel microhardness over time. J Am Dent Assoc 2003; 134 (10) 1335-1342
  • 21 Lewinstein I, Fuhrer N, Churaru N, Cardash H. Effect of different peroxide bleaching regimens and subsequent fluoridation on the hardness of human enamel and dentin. J Prosthet Dent 2004; 92 (04) 337-342
  • 22 Hegedüs C, Bistey T, Flóra-Nagy E, Keszthelyi G, Jenei A. An atomic force microscopy study on the effect of bleaching agents on enamel surface. J Dent 1999; 27 (07) 509-515
  • 23 João-Souza SH, Lussi A, Baumann T, Scaramucci T, Aranha ACC, Carvalho TS. Chemical and physical factors of desensitizing and/or anti-erosive toothpastes associated with lower erosive tooth wear. Sci Rep 2017; 7 (01) 17909
  • 24 Saffarpour M, Asgartooran B, Tahriri MR, Savadroudbari MM, Khabazkhoob M. Effect of modified 45s5 bioglass on physical and chemical properties of bleached enamel. Braz J Oral Sci 2019; 18 (01) 1-12
  • 25 Attia ML, Cavalli V, do Espírito Santo AM. et al. Effects of bleaching agents combined with regular and whitening toothpastes on surface roughness and mineral content of enamel. Photomed Laser Surg 2015; 33 (07) 378-383
  • 26 Joiner A, Schäfer F, Naeeni MM, Gupta AK, Zero DT. Remineralisation effect of a dual-phase calcium silicate/phosphate gel combined with calcium silicate/phosphate toothpaste on acid-challenged enamel in situ. J Dent 2014; 42 (Suppl. 01) S53-S59
  • 27 Hornby K, Ricketts SR, Philpotts CJ, Joiner A, Schemehorn B, Willson R. Enhanced enamel benefits from a novel toothpaste and dual phase gel containing calcium silicate and sodium phosphate salts. J Dent 2014; 42 (Suppl. 01) S39-S45
  • 28 Li X, Wang J, Joiner A, Chang J. The remineralisation of enamel: a review of the literature. J Dent 2014; 42 (Suppl. 01) S12-S20
  • 29 Worschech CC, Rodrigues JA, Martins LRM, Ambrosano GMB. In vitro evaluation of human dental enamel surface roughness bleached with 35% carbamide peroxide and submitted to abrasive dentifrice brushing. Pesqui Odontol Bras 2003; 17 (04) 342-348
  • 30 Sa Y, Sun L, Wang Z. et al. Effects of two in-office bleaching agents with different pH on the structure of human enamel: an in situ and in vitro study. Oper Dent 2013; 38 (01) 100-110