Tunisian Newborn's Cord Blood: Reference Values of Complete Blood Count and Hemoglobin Fractions

Sana Zitouni; Emna Bouatrous; Ons Laabidi; Imen Boudrigua; Dorra Chaouachi; Naima Saidani; Imen Kraiem; Amira Ayachi; Salem Abbes; Mechaal Mourali; Samia Menif; Houyem Ouragini

doi:10.1055/s-0040-1722199

American Journal of Perinatology, Table of Contents

Am J Perinatol 2022; 39(11): 1241-1247
DOI: 10.1055/s-0040-1722199

Original Article

Tunisian Newborn's Cord Blood: Reference Values of Complete Blood Count and Hemoglobin Fractions

Sana Zitouni

¹Laboratory of Molecular and Cellular Hematology, Pasteur Institute of Tunis, University of Tunis El Manar, Tunis, Tunisia

²Department of Biology, Faculty of Sciences, Carthage University, Jarzouna, Tunisia

,

Emna Bouatrous

¹Laboratory of Molecular and Cellular Hematology, Pasteur Institute of Tunis, University of Tunis El Manar, Tunis, Tunisia

,

Ons Laabidi

¹Laboratory of Molecular and Cellular Hematology, Pasteur Institute of Tunis, University of Tunis El Manar, Tunis, Tunisia

,

Imen Boudrigua

¹Laboratory of Molecular and Cellular Hematology, Pasteur Institute of Tunis, University of Tunis El Manar, Tunis, Tunisia

,

Dorra Chaouachi

¹Laboratory of Molecular and Cellular Hematology, Pasteur Institute of Tunis, University of Tunis El Manar, Tunis, Tunisia

,

Naima Saidani

³Department of Gyneco-Obstetrics, Faculty of Medicine of Tunis, University of Tunis El Manar, Hospital Habib Bougatfa, Tunis, Tunisia

,

Imen Kraiem

¹Laboratory of Molecular and Cellular Hematology, Pasteur Institute of Tunis, University of Tunis El Manar, Tunis, Tunisia

,

Amira Ayachi

³Department of Gyneco-Obstetrics, Faculty of Medicine of Tunis, University of Tunis El Manar, Hospital Habib Bougatfa, Tunis, Tunisia

,

Salem Abbes

¹Laboratory of Molecular and Cellular Hematology, Pasteur Institute of Tunis, University of Tunis El Manar, Tunis, Tunisia

,

Mechaal Mourali

³Department of Gyneco-Obstetrics, Faculty of Medicine of Tunis, University of Tunis El Manar, Hospital Habib Bougatfa, Tunis, Tunisia

,

Samia Menif

¹Laboratory of Molecular and Cellular Hematology, Pasteur Institute of Tunis, University of Tunis El Manar, Tunis, Tunisia

,

Houyem Ouragini

¹Laboratory of Molecular and Cellular Hematology, Pasteur Institute of Tunis, University of Tunis El Manar, Tunis, Tunisia

› Author Affiliations

Abstract

Objective This study was aimed to establish local reference values for hematological indices and hemoglobin (Hb) fractions in umbilical cord blood (UCB) for the northern population of Tunisia.

Study Design Our study included full-term newborns by vaginal deliveries. Hematological parameters were collected using an automated blood cell counter. The amounts of Hb fractions were measured by capillary electrophoresis of Hb. Statistical analysis was performed using R software.

Results A total of 328 cord blood samples were analyzed. Among them, 154 (male: 44.8%, female: 55.2%) were used to establish reference values. The normal reference values of complete blood count (CBC) and Hb fractions were calculated. Mean neonatal Hb was 14.75 ± 2.26 g/dL. Gestational age affects the expression of CBC values as red blood cell (RBC), Hb, hematocrit (Hct), mean corpuscular volume (MCV), white blood cell (WBC), and the Hb profile. Umbilical blood hemogram parameters and Hb profile are affected by the environment; higher in newborns from urban regions but not affected by gender ratio.

Conclusion Reference ranges of normal CBC indices and Hb fractions have been successfully established in Tunisian neonates' UCB. Our data suggest reference values that could be useful for neonatal patients' laboratory results and clinical interpretation.

Key Points

Reference values for CBC and hemoglobin fractions have been established.
Hematological reference for UCB is useful to identify hemolytic anemia cases early.
UCB hematological values are influenced by gestational age and probably by environmental factors.

Keywords

reference values - complete blood count - hemoglobin fractions - Tunisian newborn's cord blood

Full Text

References

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