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CC BY-NC-ND 4.0 · Eur J Dent 2021; 15(01): 033-038
DOI: 10.1055/s-0040-1714445
Original Article

The Role of Rice Hull Liquid Smoke in the Traumatic Ulcer Healing

Ira Arundina
1   Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Indeswati Diyatri
1   Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Tuti Kusumaningsih
1   Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Meircurius Dwi Condro Surboyo
2   Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Elita Monica
3   Undergraduate Student, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Novitasari Mira Afanda
3   Undergraduate Student, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
› Author Affiliations Funding This work was supported by Ministry of Higher Education 2020 with grant number 607/UN3.14/PT/2020.
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Abstract

Objective The aim of this study was to prove the role of rice hull liquid smoke (RH-LS) on lymphocytes, macrophages, fibroblasts, interleukin 6 (IL-6), and transforming growth factor β (TGF-β) expression during traumatic ulcer healing.

Materials and Methods The RH-LS was obtained from the pyrolysis process. Traumatic ulcers were made 10 mm along the labial fornix incisive inferior of Wistar rat using a round stainless-steel blade. In control group, traumatic ulcers were treated using sterile water, and meanwhile in experimental group were treated using RH-LS once a day for 3, 5, and 7 days. After treatment, animal was terminated and their labial fornix incisive inferior tissues were biopsy and stained using hematoxylin and eosin staining to determine lymphocytes, macrophages, and fibroblasts. The IL-6 and TGF-β expressions were analyzed used immunohistochemistry staining.

Result The lymphocytes, macrophages, and fibroblasts were higher in the RH-LS group for 3-, 5-, and 7-day treatment (p < 0.05). The IL-6 expression was higher only in the 5-day treatment, and the TGF-β expression was higher in the 3- and 7-day treatment.

Conclusion The RH-LS able to accelerated the traumatic ulcer healing by increasing the number of lymphocytes, macrophages, fibroblasts, IL-6, and TGF-β expression.

Keywords

traumatic ulcer - fibroblast - macrophage - lymphocyte - rice hull - liquid smoke - IL-6 and TGF-β


Publication History

Article published online:
10 August 2020

© 2020. European Journal of Dentistry. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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