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CC BY-NC-ND 4.0 · Eur J Dent 2021; 15(01): 008-012
DOI: 10.1055/s-0040-1714039
Original Article

Role of Hydroxyapatite and Ellagic Acid in the Osteogenesis

Agung Satria Wardhana
1   Department of Dental Material, Faculty of Dentistry, Universitas Lambung Mangkurat, Banjarmasin, Indonesia
,
Intan Nirwana 
2   Department of Dental Material, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Hendrik Setia Budi 
3   Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Meircurius Dwi Condro Surboyo 
4   Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
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Abstract

Objective Ellagic acid (EA), a phenolic antioxidant, has benefits in bone health and wound healing. The combination of EA and hydroxyapatite (HA) (EA-HA) is expected to increase osteogenesis. The aim of this study was to analyze osteogenesis after application of EA-HA according to the number of osteoblasts and osteoclasts in the bone and the expression of the receptor activator of nuclear factor kappa-β ligand (RANKL), osteoprotegerin (OPG), and osteocalcin (OCN) protein.

Materials and Methods Thirty Wistar rats were assessed with bone defects created in the left femur. The defects were filled with EA-HA and then sutured. Control groups were filled with polyethylene glycol (PEG) or HA. Each group was sacrificed either 7 or 14 days after treatment.

Results The defects filled with EA-HA exhibited the highest number of osteoblasts and the greatest expression of OPG and OCN at both day 7 and day 14 (p = 0.000). Conversely, treatment with EA-HA resulted in lower numbers of osteoclasts and reduced RANKL staining at both time points (p = 0.000).

Conclusions EA-HA can increase osteogenesis in bone defects by increasing the number of osteoblasts and the expression of OPG and OCN.

Keywords

osteogenesis - hydroxyapatite - ellagic acid - osteocalcin - RANKL - OPG


Publication History

Article published online:
20 July 2020

© 2020. European Journal of Dentistry. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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