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CC BY-NC-ND 4.0 · Journal of Health and Allied Sciences NU 2016; 06(03): 06-09
DOI: 10.1055/s-0040-1708653
Original Article

Quantitative evaluation of DNA from the tooth pulp exposed to varying temperatures

Aisha N. Ibrahim
1   Former Student, Department of prosthodontics, A.B. Shetty Memorial Institute of Dental Sciences, Mangalore
,
Vinaya Bhat
2   Professor, Department of prosthodontics, A.B. Shetty Memorial Institute of Dental Sciences, Mangalore
,
Shilpa M. Shenoy
3   Central Research Laboratory, Department of Microbiology, K.S. Hegde Medical Academy, Nitte University, Mangalore
,
Veena A. Shetty
4   Associate Professor, Department of Microbiology, K.S. Hegde Medical Academy, Nitte University, Mangalore
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Abstract

Context: During natural calamities like fire accidents, many times the human body gets charred beyond recognition. Tooth, being the hardest structure in the human body protects the pulp within from such accidents. Forensic identification through the pulpal DNA could be avery useful tool in such situations.

Aim: This study was designed to evaluate the quantity of DNA obtained from the pulp when exposed to varying temperatures.

Methods and Material: Extracted teeth were subjected to the following temperatures:-80°C,37°C,100°C, 200°C, 300°C, 500°C and 1000°C. Pulp from these teeth were retrieved by horizontally sectioning the teeth using a fine needle diamond point in a high speed air rotor, followed by extraction of DNA with the HipuraA™ Forensic Genomic DNA purification spink it. The samples were quantified using personal computer(PC) based double beam spectro photo meter. Kruskal Wallis test and Mann WhitneyU test were used for statistical analysis.

Results: Statistically significant difference (p<0.005) was seen in the quantity of DNA obtained from pulp subjected to higher temperatures as compared to oral temperature. However, there was no significant difference (p>0.05) in the quantity of DNA obtained from teeth subjected to-80°C

Conclusions: Increase in temperature decreases the amount of DNA from the tooth pulp where as a decrease in temperature does not cause any change in the quantity of DNA.

Keywords

DNA - temperatures - forensic dentistry - pulp - tooth


Publikationsverlauf

Eingereicht: 16. Dezember 2015

Angenommen: 28. Juni 2016

Artikel online veröffentlicht:
22. April 2020

© .

Thieme Medical and Scientific Publishers Private Ltd.
A-12, Second Floor, Sector -2, NOIDA -201301, India

 

  • References

  • 1 Cameron JM, Sims BG. Forensic dentistry. Churchill Livingstone 1973;23-25
  • 2 Rothwell BR. Principles of dental identification. Dent Clin North Am 2001;45:2253-70
  • 3 Malaver PC, Yunis JJ. Different dental tissues as source of DNA for human identification in forensic cases.Croatian medical journal 2003;44:306-309
  • 4 Girish KL, Rahman FS, Tippu SR. Dental DNA fingerprinting in identification of human remains. Journal of forensic dental sciences 2010;2:63-68
  • 5 Muruganandhan J, Sivakumar G. Practical aspects of DNA-based forensic studies in dentistry. Journal of forensic dental sciences 2011;3:38-45
  • 6 Smith BC, Fisher DL, Weedn VW, Warnock GR, Holland MM.A systematic approach to the sampling of dental DNA.Journal of forensic sciences 1993; 38:1194-1209.
  • 7 Lessig, Edelmann J. Individualisation of dental tissue--an aid for odontological identification? The Journal of forensic odontostomatology 1995;13:1-3
  • 8 Vemuri S, Ramya R, Rajkumar K, Rajashree P. Influence of various environmental conditions on DNA isolation from dental pulp for sex determination using polymerase chain reaction. SRM Journal of Research in Dental Sciences 2012;3:231-235
  • 9 Alves da Silva RH, Quiezi R, Pereira Bertolacini CD, MacielCarvalho SP, da Silva Gasque KC, de Almeida-e-Silva CT, RibeiroBicudo LA. Human identification analysis using PCR from the root portion of dental elements under different conditions of temperature and exposure time. RSBO RevistaSul-Brasileira de Odontologia 2012;967-73
  • 10 Sweet DJ, Sweet CH. DNA analysis of dental pulp to link incinerated remains of homicide victim to crime scene. Journal of forensic sciences 1995; 40:310-314.
  • 11 Burger J, Hummel S, Herrmann B, Henke W. DNA preservation: a microsatellite-DNA study on ancient skeletal remains. Electrophoresis 1999; 20:1722-1728.
  • 12 Pötsch, L., et al. Application of DNA techniques for identification using human dental pulp as a source of DNA. International journal of legal medicine 1992; 105.3:139-143.
  • 13 Moretti TR, Baumstark AL, Defenbaugh DA, Keys KM, Smerick JB, Budowle BJ. Validation of STRs for forensic usage: Performance testing of fluorescent multiplex STR systems and analysis of authentic and simulated forensic samples. Forensic Sci. 2001; 46:647–60.