Download PDF
CC BY-NC-ND 4.0 · Journal of Health and Allied Sciences NU 2011; 01(01/03): 15-18
DOI: 10.1055/s-0040-1703513
Original Article

EFFECT OF ELECTRON BEAM RADIATION ON HEMATOPOIETIC CELLS OF SWISS ALBINO MICE

Suchetha Kumari N
Department of Biochemistry, K.S. Hegde Medical Academy, Nitte University, Mangalore
,
Madhu L.N
Central Research Laboratory, Nitte University, Mangalore
› Author Affiliations
› Further Information
Also available at
  PDF Download Permissions and Reprints

Abstract

Ionizing radiation which results in the free radical formation and it leads to damage of biological macromolecules such as DNA, proteins, lipids. 36 male Swiss albino mice were used for survival assay, to find out the lethal dose of Electron Beam Radiation. It was found to be 10Gy was the lethal dose for mice. Different dosages (4Gy, 6Gy and 8Gy) of electron beam radiation were used to study the micronucleus formation in irradiated mice. The results showed micronucleus formation will increase linearly with radiation dosage.

Keywords

Electron beam radiation - LD50 - Micronucleus


Publication History

Article published online:
20 February 2020

© .

Thieme Medical and Scientific Publishers Private Ltd.
A-12, Second Floor, Sector -2, NOIDA -201301, India

 

  • References

  • 1 Boreck C. Antioxidatnts and radiation therapy. J. Nutr 2004; 134:3207- 3209.
  • 2 Little JB. Cellular, molecular and carcinogenic effects of radiation. Hematol Oncol Clin North Am 1993; 7:337-352.
  • 3 Erselcan T, Sungu S, Ozdemir S, Turgut B, Dogan D, Ozdemir O. Iodine-131 treatment and chromosomal damage: in-vivo dose-effect relationship. Eur J Nucl Med Mole Imag 2004; 31:676-84.
  • 4 Coia LR, Moyland DJ. Introduction to Clinical Radiation Oncology. 1998; Medical Physics. Publishing, Madison, WI.
  • 5 Reily PA. Free radicals in biology: oxidative stress and the effect of ionizing radiation. Int J Radiat Biol 1994; 65:27–33.
  • 6 Uma Devi P, Nagarathnam A, Sathish Rao BS. Introduction to radiation biology. 2000; B.I. Churchill Livingstone Pvt. Ltd., New Delhi.
  • 7 Loevinger, R, Karzmark CJ, Weiss Bluth M. Radiation therapy with high Energy electrons. Part I. Physical considerations-10 to 60 Mev. Radiology 1961; 77:906- 927.
  • 8 Ghosh MN. Toxicity studies: Fundamentals of experimental pharmacology. (Ghosh MN., ed.), 1984; pp. 153-158, Scientific Book Agency: Calcutta, India.
  • 9 Schmidt W. The Micronucleus test. Mutation Research 1975; 31:9-15.
  • 10 Shilpa S. Puthran, Sudha K, Gayathri M. Rao, Beena VS. Oxidative Stress And Low Dose Ionizing Radiation. Indian J Physiol Pharmacol 2009; 53(2):181–184
  • 11 Jagetia GC, Baliga MS, Malagi KJ, Kamath MS. The Evaluation of the Radioprotective Effect of Triphala (an Ayurvedic rejuvenating drug) in the mice exposed to ã-radiation. Phytomedicine 2002; 9:99-108.
  • 12 Jagetia GC, Baliga MS. An Ayurvedic Herbal Drug Imparts Protection to the Mice against the lethal Effects of Gamma-radiation: a Preliminary Study. Nahrung 2002; 46:332-6.
  • 13 Bond VP, Fliedner TM, Archambeau JO. Mammalian Radiation Lethality. 1965; Academic Press, New York, USA.
  • 14 Prabha T, Amit Kumar, Balakrishnan S, Kushwaha HS, Mishra KP. Radiationinduced micronucleus formation and DNA damage in human lymphocytes and their prevention by antioxidant thiols. Mutation research 2009; 676:262-68.