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CC BY-NC-ND 4.0 · Eur J Dent 2020; 14(01): 123-127
DOI: 10.1055/s-0040-1702900
Original Article

Concanavalin A Enhanced Proliferation and Osteogenic Differentiation of Dental Pulp Stem Cells

Ketut Suardita
1   Department of Conservative Dentistry, Faculty of Dental Medicine, Universitas Airlangga, Jawa Timur, Indonesia
,
Ira Arundina
2   Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Jawa Timur, Indonesia
,
Udijanto Tedjosasongko
3   Department of Pediatric Dentistry, Faculty of Dental Medicine, Universitas Airlangga, Jawa Timur, Indonesia
,
Anita Yuliati
4   Department of Dental Material, Faculty of Dental Medicine, Universitas Airlangga, Jawa Timur, Indonesia
,
Harry Huiz Peeters
5   Laser Research Center in Dentistry, Bandung, Indonesia
,
I Komang Evan Wijaksana
6   Department of Periodontics, Faculty of Dental Medicine, Universitas Airlangga, Jawa Timur, Indonesia
,
Meircurius Dwi Condro Surboyo 
7   Department of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Indonesia
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Abstract

Objective Dental pulp stem cells (DPSCs) can be used as a component in the formation of regenerative dentine during direct pulp capping therapy. Concanavalin A (ConA) is a type of lectin with a molecular weight of 26 kDa derived from the Canavalia ensiformis plant. Lectins possess strong proliferation and differentiation abilities in various animal cells including lymphocytes, osteoblasts, and chondrocytes. The aim of study was to determine the effect of ConA on the proliferation and osteogenic differentiation of DPSCs in vitro.

Materials and Methods In this in vitro study, DPSCs were isolated from third molars before ConA induction was performed at concentrations of 5 and 10 μg/mL. The proliferation assay was determined by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Osteogenic differentiation was determined by means of mineralization.

Statistical Analysis Data were analyzed using analysis of variance and a Student’s t-test. The p-value was set at 0.05.

Results The addition of 5 and 10 µg/mL of ConA to DPSCs can significantly increase the proliferation and osteogenic differentiation of DPSCs (p ≤0.05).

Conclusion ConA can increase the proliferation and osteogenic differentiation of DPSCs.

Keywords

concanavalin A - dental pulp - differentiation - proliferation - stem cell


Publication History

Article published online:
13 March 2020

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Thieme Medical and Scientific Publishers Private Ltd.
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