PDF herunterladen
CC BY-NC-ND 4.0 · Int Arch Otorhinolaryngol 2020; 24(02): e206-e210
DOI: 10.1055/s-0039-1697996
Original Research
Thieme Revinter Publicações Ltda Rio de Janeiro, Brazil

Study of Dosage-Dependent Effects of Cytostatic Drugs Using a Fibroblast Cell Culture of the Human Nasal Mucosa

Vasily Yartsev 
1   Department of Lacrimal Pathology, Scientific Research Institute of Eye Diseases, Moscow, Russian Federation
,
Anna Gabashvili
2   Fundamental Laboratory, Scientific Research Institute of Eye Diseases, Moscow, Russian Federation
3   Cell Laboratory, Naucno-issledovatel'skij Institut Biomedicinskoj Himii Imeni V N Orehovica, Moscow, Russian Federation
,
Eugenia Atkova
1   Department of Lacrimal Pathology, Scientific Research Institute of Eye Diseases, Moscow, Russian Federation
,
Pavel Melnikov
4   Cell Laboratory, Serbsky Federal Medical Research Centre for Psychiatry and Narcology, Moscow, Russian Federation
,
Tatiana Nesterova
5   Cell Laboratory, Scientific Research Institute of Eye Diseases, Moscow, Russian Federation
› Institutsangaben
Weitere Informationen

Publikationsverlauf

26. November 2018

19. August 2019

Publikationsdatum:
28. Januar 2020 (online)

   Lizenzen und Reprints

Abstract

Introduction Knowing a concentration at which cytostatic drugs are toxic for the nasal fibroblasts will enable the use cytostatic drugs in the clinical practice to prevent excessive cicatrization.

Objective To determine the cytostatic concentrations of mitomycin С, doxorubicin, and 5-fluorouracil affecting nasal mucosa fibroblasts.

Methods We obtained material during an endonasal dacryocystorhinostomy with the patient's informed consent. The cells were cultivated. Second- to fourth-passage cells were used in the experiments. The cells were stained for vimentin and cluster of differentiation 90 (CD90). An MTS test 3 (3-(4,5-dimethylthiazole-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium); cell viability test was performed.

Results The cytostatic drugs have a toxic effect on cultivated fibroblasts of the nasal mucosa. This effect is dose-dependent. In terms of reducing the level of tissue fibrotisation in the nasal cavity, the most justified approach is to carry out an experimental study of the effect of mitomycin C, doxorubicin, and 5-fluorouracil at the concentrations of 0.25 mg/ml, 0.25 mg/ml, and 12.5 mg/ml respectively.

Conclusion The authors argue that it is inappropriate to use these cytostatic drugs to conduct studies with the goal of analyzing their antifibrotic effect on the nasal mucosa at concentrations that are either lower or higher than the aforementioned ones.

Keywords

fibroblasts - fibrosis - mitomycin C - doxorubicin - 5-fluorouracil - cytostatic drugs
 

  • References

  • 1 Huang J, Malek J, Chin D. , et al. Systematic review and meta-analysis on outcomes for endoscopic versus external dacryocystorhinostomy. Orbit 2014; 33 (02) 81-90 . Doi: 10.3109/01676830.2013.842253
    CrossrefPubMedGoogle Scholar
  • 2 Syed MI, Head EJ, Madurska M, Hendry J, Erikitola OC, Cain AJ. Endoscopic primary dacryocystorhinostomy: are silicone tubes needed? Our experience in sixty three patients. Clin Otolaryngol 2013; 38 (05) 406-410 . Doi: 10.1111/coa.12152
    CrossrefPubMedGoogle Scholar
  • 3 Dave TV, Mohammed FA, Ali MJ, Naik MN. Etiologic analysis of 100 anatomically failed dacryocystorhinostomies. Clin Ophthalmol 2016; 10: 1419-1422 . Doi: 10.2147/OPTH.S113733
    CrossrefPubMedGoogle Scholar
  • 4 Lin GC, Brook CD, Hatton MP, Metson R. Causes of dacryocystorhinostomy failure: External versus endoscopic approach. Am J Rhinol Allergy 2017; 31 (03) 181-185 . Doi: 10.2500/ajra.2017.31.4425
    CrossrefPubMedGoogle Scholar
  • 5 Ali MJ, Mariappan I, Maddileti S, Ali MH, Naik MN. Mitomycin C in dacryocystorhinostomy: the search for the right concentration and duration--a fundamental study on human nasal mucosa fibroblasts. Ophthal Plast Reconstr Surg 2013; 29 (06) 469-474 . Doi: 10.1097/IOP.0b013e3182a23086
    CrossrefPubMedGoogle Scholar
  • 6 Jawad M, Ali Z, Tariq S, Qayum I, Aftab H. Effect of Intraoperative Mitomycin-C Application in Outcome of External Dacryocystorhinostomy. J Ayub Med Coll Abbottabad 2015; 27 (03) 598-600
    PubMedGoogle Scholar
  • 7 Mukhtar SA, Jamil AZ, Ali Z. Efficacy of external dacryocystorhinostomy (DCR) with and without mitomycin-C in chronic dacryocystitis. J Coll Physicians Surg Pak 2014; 24 (10) 732-735 . Doi: 10.2014/JCPSP.732735
    PubMedGoogle Scholar
  • 8 Atkova EL, Fedorov AA, Root AO, Iartsev SD, Krakhovetsky NN, Yartsev VD. Causes of unsatisfactory results of the use of mitomycin-C in endoscopic endonasal dacryocystorhinostomy. Saudi J Ophthalmol 2017; 31 (03) 150-155 . Doi: 10.1016/j.sjopt.2017.05.007
    CrossrefPubMedGoogle Scholar
  • 9 Ozsutcu M, Balci O, Tanriverdi C, Demirci G. Efficacy of adjunctive mitomycin C in transcanalicular diode laser dacryocystorhinostomy. Eur Arch Otorhinolaryngol 2017; 274 (02) 873-877 . Doi: 10.1007/s00405-016-4308-7
    CrossrefPubMedGoogle Scholar
  • 10 Abraham LM, Selva D, Casson R, Leibovitch I. The clinical applications of fluorouracil in ophthalmic practice. Drugs 2007; 67 (02) 237-255
    CrossrefPubMedGoogle Scholar
  • 11 Bakri K, Jones NS, Downes R, Sadiq SA. Intraoperative fluorouracil in endonasal laser dacryocystorhinostomy. Arch Otolaryngol Head Neck Surg 2003; 129 (02) 233-235
    PubMedGoogle Scholar
  • 12 Watts P, Ram AR, Nair R, Williams H. Comparison of external dacryocystorhinostomy and 5-fluorouracil augmented endonasal laser dacryocystorhinostomy. A retrospective review. Indian J Ophthalmol 2001; 49 (03) 169-172
    PubMedGoogle Scholar
  • 13 Sakagami H, Okudaira N, Masuda Y. , et al. Induction of Apoptosis in Human Oral Keratinocyte by Doxorubicin. Anticancer Res 2017; 37 (03) 1023-1029 . Doi: 10.21873/anticanres.11412
    Google Scholar