CC BY-NC-ND 4.0 · Eur J Dent 2019; 13(01): 058-063
DOI: 10.1055/s-0039-1688527
Original Article
Dental Investigation Society

Human Umbilical Cord Mesenchymal Stem-Cell Therapy to Increase the Density of Osteoporotic Mandibular Bone

Nike Hendrijantini
1   Department of Prosthodontic, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Poedjo Hartono
2   Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Muhammad Dimas Aditya Ari
1   Department of Prosthodontic, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Fedik Abdul Rantan
3   Department of Microbiology and Virology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia
› Author Affiliations
Further Information

Publication History

Publication Date:
06 June 2019 (online)

Abstract

Objective The aim of this study is to evaluate the feasibility of human umbilical cord mesenchymal stem-cell (hUCMSC) therapy in increasing osteoporotic mandibular bone density in a rat model by determining changes in alkaline phosphatase (ALP), osteocalcin, type 1 collagen, and trabecular bone area after treatment.

Materials and Methods This research adopted an experimental posttest-only control group design. Thirty female Wistar rats were randomly divided into six groups, namely, a control group with rats postsham surgery (T1), osteoporotic model postovariectomy rats (T2), postovariectomy rats 4 weeks after gelatin injection (T3), postovariectomy rats 8 weeks after gelatin injection (T4), postovariectomy rats 4 weeks after hUCMSC injection (T5), and postovariectomy rats 8 weeks after hUCMSC injection (T6). The rats were all sacrificed for histological and immunohistochemical examinations of ALP, osteocalcin, type 1 collagen, and trabecular bone area.

Results Increased expression of ALP, type 1 collagen, and osteocalcin, as well as increased trabecular bone area, was observed in the treatment groups compared with that in the osteoporotic groups.

Conclusion hUCMSCs produce significant osteogenic effects and increase osteoporotic mandibular bone density in the animal model. Increases in bone density are demonstrated by the higher levels of ALP, osteocalcin, and type 1 collagen, as well as increases in the trabecular bone area.

 
  • References

  • 1 Schimmel M, Müller F, Suter V, Buser D. Implants for elderly patients. Periodontol 2000 2017; 73 (01) 228-240
  • 2 Guyton AC, Hall JE. Text Book of Medical Physiology. 11th ed.. Jakarta: EGC Medical Publisher; 2007: 1070-6
  • 3 Leslie WD, Tsang JF, Caetano PA, Lix LM. Manitoba Bone Density Program. Effectiveness of bone density measurement for predicting osteoporotic fractures in clinical practice. J Clin Endocrinol Metab 2007; 92 (01) 77-81
  • 4 Kling JM, Clarke BL, Sandhu NP. Osteoporosis prevention, screening, and treatment: a review. J Womens Health (Larchmt) 2014; 23 (07) 563-572
  • 5 Misch CE. Rationale for dental implants. In: Contemporary Implant Dentistry. 3rd ed.. St. Louis, Canada: Mosby Inc.; 2008: 3-21
  • 6 Romanov YA, Svintsitskaya VA, Smirnov VN. Searching for alternative sources of postnatal human mesenchymal stem cells: candidate MSC-like cells from umbilical cord. Stem Cells 2003; 21 (01) 105-110
  • 7 Hendrijantini N, Kusumaningsih T, Rostiny R, Mulawardhana P, Danudiningrat CP, Rantam FA. A potential therapy of human umbilical cord mesenchymal stem cells for bone regeneration on osteoporotic mandibular bone. Eur J Dent 2018; 12 (03) 358-362
  • 8 Rutkovskiy A, Stensløkken KO, Vaage IJ. Osteoblast differentiation at a glance. Med Sci Monit Basic Res 2016; 22: 95-106
  • 9 Florencio-Silva R, Sasso GR, Sasso-Cerri E, Simões MJ, Cerri PS. Biology of bone tissue: structure, function, and factors that influence bone cells. BioMed Res Int 2015; 2015: 421746
  • 10 Blair HC, Larrouture QC, Li Y. et al. Osteoblast differentiation and bone matrix formation in vivo and in vitro. . Tissue Eng Part B Rev 2017; 23 (03) 268-280
  • 11 Orimo H. The mechanism of mineralization and the role of alkaline phosphatase in health and disease. J Nippon Med Sch 2010; 77 (01) 4-12
  • 12 Kini U, Nandeesh BN. Physiology of bone formation, remodeling, and metabolism. In: Fogelman I, Gnanasegaran G, Van der Wall H. eds. Radionuclide and Hybrid Bone Imaging. Berlin: Springer; 2012: 29-57
  • 13 Pino AM, Rosen CJ, Rodríguez JP. In osteoporosis, differentiation of mesenchymal stem cells (MSCs) improves bone marrow adipogenesis. Biol Res 2012; 45 (03) 279-287
  • 14 Prall WC, Haasters F, Heggebö J. et al. Mesenchymal stem cells from osteoporotic patients feature impaired signal transduction but sustained osteoinduction in response to BMP-2 stimulation. Biochem Biophys Res Commun 2013; 440 (04) 617-622
  • 15 Ma L, Aijima R, Hoshino Y. et al. Transplantation of mesenchymal stem cells ameliorates secondary osteoporosis through interleukin-17-impaired functions of recipient bone marrow mesenchymal stem cells in MRL/lpr mice. Stem Cell Res Ther 2015; 6: 104
  • 16 Li C, Wei G, Gu Q, Wang Q, Tao S, Xu L. Proliferation and differentiation of rat osteoporosis mesenchymal stem cells (MSCs) after telomerase reverse transcriptase (TERT) transfection. Med Sci Monit 2015; 21: 845-854
  • 17 Kaveh K, Ibrahim R, Bakar M, Ibrahim T. Mesenchymal stem cells, osteogenic lineage and bone tissue engineering: a review. J Anim Vet Adv 2011; 10: 2317-2330
  • 18 Tabata Y. Tissue regeneration based on drug delivery technology. In: Ashammakhi N, Ferretti P. eds. Topics in Tissue Engineering. Vol. 1. Tamahe, Finlandia: University of Oulu; 2003: 1-32
  • 19 Marie PJ, Kassem M. Osteoblasts in osteoporosis: past, emerging, and future anabolic targets. Eur J Endocrinol 2011; 165 (01) 1-10
  • 20 Ichioka N, Inaba M, Kushida T. et al. Prevention of senile osteoporosis in SAMP6 mice by intrabone marrow injection of allogeneic bone marrow cells. Stem Cells 2002; 20 (06) 542-551
  • 21 Wang Z, Goh J, Das De S. et al. Efficacy of bone marrow-derived stem cells in strengthening osteoporotic bone in a rabbit model. Tissue Eng 2006; 12 (07) 1753-1761