RSS-Feed abonnieren
![](/products/assets/desktop/img/oa-logo.png)
DOI: 10.1055/s-0039-1683405
Effect of Diabetes on the Male Reproductive System—A Histomorphological Study
Publikationsverlauf
12. Dezember 2018
02. Februar 2019
Publikationsdatum:
20. März 2019 (online)
![](https://www.thieme-connect.de/media/10.1055-s-00040444/201901/lookinside/thumbnails/10-1055-s-0039-1683405_1873-1.jpg)
Abstract
Introduction Type 1 diabetes is an autoimmune disorder characterized by lack of insulin production by the β cells of the pancreas. This lack of insulin causes a variety of systemic effects on the metabolism of the body, one of which is reproductive dysfunction. The present study investigates the effects of diabetes on the male reproductive system of streptozotocin (STZ)-induced diabetic rats.
Material and Methods A total of 18 adult male Wistar rats weighing ∼ between 250 and 300 g were included in the present study. The animals were divided into normal and diabetic groups. The diabetic group was further subdivided into 2 subgroups with durations of 24 and 48 days. A single dose of STZ (40 mg/kg body weight) was administrated intraperitoneally to the animals of the diabetic group. After the planned duration, the testes and epididymides were dissected, and their gross weight was measured. The tissues were then processed for histological study.
Results The gross weight of the testes and epididymides in diabetic rats at 24 and 48 days showed a decrease in comparison to the control. (p < 0.01 for testes and epididymides).
Diabetic animals presented a significant decrease in the diameter of the seminiferous tubules compared with the control group (p < 0.01). The epididymides in the diabetic groups showed a considerable reduction in the tubular surface area compared with the control group (p < 0.01). There was also a reduction in the mean diameter, which was measured using the maximum and minimum diameter of the tubules (p < 0.01).
Conclusion The present study is an insight into the adverse effects that diabetes can have on the tissue structure of the testes, of the epididymides, and ultimately on the process of spermatogenesis.
-
References
- 1 Wild S, Roglic G, Green A, Sicree R, King H. Global prevalence of diabetes: estimates for the year 2000 and projections for 2030. Diabetes Care 2004; 27 (05) 1047-1053
- 2 Long L, Wang J, Lu X. , et al. Protective effects of scutellarin on type II diabetes mellitus-induced testicular damages related to reactive oxygen species/Bcl-2/Bax and reactive oxygen species/microcirculation/staving pathway in diabetic rat. J Diabetes Res 2015; 2015: 252530 . Doi: 10.1155/2015/252530
- 3 Fedder J, Kaspersen MD, Brandslund I, Højgaard A. Retrograde ejaculation and sexual dysfunction in men with diabetes mellitus: a prospective, controlled study. Andrology 2013; 1 (04) 602-606
- 4 Ramaswamy S, Weinbauer GF. Endocrine control of spermatogenesis: Role of FSH and LH/ testosterone. Spermatogenesis 2015; 4 (02) e996025
- 5 Ballester J, Muñoz MC, Domínguez J, Rigau T, Guinovart JJ, Rodríguez-Gil JE. Insulin-dependent diabetes affects testicular function by FSH- and LH-linked mechanisms. J Androl 2004; 25 (05) 706-719
- 6 Maritim AC, Sanders RA, Watkins III JB. Diabetes, oxidative stress, and antioxidants: a review. J Biochem Mol Toxicol 2003; 17 (01) 24-38
- 7 Shrilatha B. , Muralidhara. Early oxidative stress in testis and epididymal sperm in streptozotocin-induced diabetic mice: its progression and genotoxic consequences. Reprod Toxicol 2007; 23 (04) 578-587
- 8 Khaneshi F, Nasrolahi O, Azizi S, Nejati V. Sesame effects on testicular damage in streptozotocin-induced diabetes rats. Avicenna J Phytomed 2013; 3 (04) 347-355
- 9 Johnsen SG. Testicular biopsy score count--a method for registration of spermatogenesis in human testes: normal values and results in 335 hypogonadal males. Hormones 1970; 1 (01) 2-25
- 10 La Vignera S, Condorelli R, Vicari E, D'Agata R, Calogero AE. Diabetes mellitus and sperm parameters. J Androl 2012; 33 (02) 145-153
- 11 Guneli E, Tugyan K, Ozturk H, Gumustekin M, Cilaker S, Uysal N. Effect of melatonin on testicular damage in streptozotocin-induced diabetes rats. Eur Surg Res 2008; 40 (04) 354-360
- 12 Altay B, Cetinkalp S, Doganavşargil B, Hekimgil M, Semerci B. Streptozotocin-induced diabetic effects on spermatogenesis with proliferative cell nuclear antigen immunostaining of adult rat testis. Fertil Steril 2003; 80 (02) (Suppl. 02) 828-831
- 13 Rohrbach DH, Martin GR. Structure of basement membrane in normal and diabetic tissue. Ann N Y Acad Sci 1982; 401: 203-211
- 14 Soudamani S, Yuvaraj S, Malini T, Balasubramanian K. Experimental diabetes has adverse effects on the differentiation of ventral prostate during sexual maturation of rats. Anat Rec A Discov Mol Cell Evol Biol 2005; 287 (02) 1281-1289
- 15 Sancheti S, Bafna M, Seo S. Anti hyperglycemic, anti hyperlipidemic, and antioxidant effects of Chaenomelessinensis fruit extract in streptozotocin induced diabetic rats. Eur Food Res Technol 2010; 231: 415-421
- 16 Amaral S, Oliveira PJ, Ramalho-Santos J. Diabetes and the impairment of reproductive function: possible role of mitochondria and reactive oxygen species. Curr Diabetes Rev 2008; 4 (01) 46-54
- 17 Bauché F, Fouchard MH, Jégou B. Antioxidant system in rat testicular cells. FEBS Lett 1994; 349 (03) 392-396
- 18 Kushwaha S, Jena GB. Telmisartan ameliorates germ cell toxicity in the STZ-induced diabetic rat: studies on possible molecular mechanisms. Mutat Res 2013; 755 (01) 11-23
- 19 Singh VK, Lal B. Pro-steroidogenic and pro-spermatogenic actions of nitric oxide (NO) on the catfish, Clarias batrachus: An in vivo study. Gen Comp Endocrinol 2017; 242: 1-10
- 20 Rodriguez-Castelan J, Mendez-Tepepa M, Carrillo-Portillo Y. , et al. Hypothyroidism reduces the size of ovarian follicles and promotes hypertrophy of periovarian fat with infiltration of macrophages in adult rabbits. BioMed Res Int 2017; 2017: 3795950
- 21 Burul-Bozkurt N, Pekiner C, Kelicen P. Diabetes alters aromatase enzyme levels in gonadal tissues of rats. Naunyn Schmiedebergs Arch Pharmacol 2010; 382 (01) 33-41
- 22 Navarro-Casado L, Juncos-Tobarra MA, Cháfer-Rudilla M, de Onzoño LÍ, Blázquez-Cabrera JA, Miralles-García JM. Effect of experimental diabetes and STZ on male fertility capacity. Study in rats. J Androl 2010; 31 (06) 584-592
- 23 Oksanen A. Testicular lesions of streptozotocin diabetic rats. Horm Res 1975; 6 (03) 138-144