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Tierarztl Prax Ausg K Kleintiere Heimtiere 2018; 46(06): 393-398
DOI: 10.1055/s-0038-1677403
Originalartikel – Original Articles
Georg Thieme Verlag KG Stuttgart · New York

Blood reference intervals for rabbits (Oryctolagus cuniculus) from routine diagnostic samples

Bestimmung von Blutreferenzwerten für Kaninchen (Oryctolagus cuniculus) aus Routinediagnostikproben
Christoph Leineweber
1   LABOKLIN GmbH & Co. KG, Bad Kissingen, Germany
,
Elisabeth Müller
1   LABOKLIN GmbH & Co. KG, Bad Kissingen, Germany
,
Rachel E. Marschang
1   LABOKLIN GmbH & Co. KG, Bad Kissingen, Germany
› Author Affiliations
Further Information

Publication History

Received: 06 July 2018

Accepted after revision: 29 August 2018

Publication Date:
18 January 2019 (online)

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Summary

Objective: The goal of this study was to establish reference intervals for blood parameters in rabbits based on data from routine diagnostic samples. Materials and methods: Data sets from 1679–2039 values for clinical chemistry and 937–1559 values for hematological parameters were evaluated using the statistical program Reference Limit Estimator 20151017. Results: The following reference intervals were calculated for hematology: erythrocytes 4.37–7.43 × 1012 cells/l; hematocrit 0.28–0.48 l/l; hemoglobin 89.63–153.82 g/l; leucocytes 2.71–12.23 × 109 cells/l; neutrophils 0.87–7.82 × 109 cells/l; lymphocytes 0.36–6.58 × 109 cells/l; monocytes 0.08–1.71 × 109 cells/l; eosi nophils 0.07–0.19 × 109 cells/l; basophils 0.06–1.1 × 109 cells/l; thrombocytes 225.45–905.3 × 109 cells/l. Calculated intervals for clinical chemistries were: alkaline phosphatase 9.05–94.68 U/l; aspartataminotransferase 3.75–32.44 U/l; creatine kinase 1.63–559.53 U/l; γ-glutamyltransferase 2.5–14.46 U/l; glutamatdehydrogenase 0.68–14.78 U/l; fructosamin 248.08–501.43 µmol/l; bile acid 0.76–19.63 μmol/l; total protein 48.66–73.64 g/l; urea 2.63–10.28 mmol/l; creatinine 51.38–154.35 µmol/l; calcium 3.02–4.3 mmol/l; magnesium 0.66–1.51 mmol/l; phosphorus 0.54–2.18 mmol/l; sodium 132.61– 154.0 mmol/l and potassium 3.52–6.04 mmol/l. Conclusion and clinical relevance: The calculation of intervals based on a large number of routine diagnostic samples allows the establishment of labora tory specific reference intervals without the use of experimental animals.

Zusammenfassung

Ziel dieser Studie war die Etablierung von Referenzwerten für Blut-parameter beim Kaninchen aus Daten, die von Routineeinsendungen stammten. Material und Methoden: Die Auswertung erfolgte mit dem Statistikprogram „Reference Limit Estimator 20151017“ und berücksichtigte 1679–2039 Resultate von klinisch-chemischen sowie 937–1559 Resultate von hämatologischen Parametern. Ergebnisse: Für die hämatologischen Parameter wurden folgende Referenzwerte berechnet: Erythrozyten 4,37–7,43 × 1012 Zellen/l; Hämatokrit 0,28–0,48 l/l; Hämoglobin 89,63–153,82 g/l; Leukozyten 2,71–12,23 × 109 Zellen/l; Neutrophile 0,87–7,82 × 109 Zellen/l; Lym phozyten 0,36–6,58 × 109 Zellen/l; Monozyten 0,08–1,71 × 109 Zellen/l; Eosi nophile 0,07–0,19 × 109 Zellen/l; Basophile 0,06–1,1 × 109 Zellen/l; Thrombozyten 225,45–905,3 × 109 Zellen/l. Für die klinisch-chemi schen Parameter ergaben sich folgende Referenzbereiche: alkalische Phosphatase 9,05–94,68 U/l; Aspartat-Aminotransferase 3,75–32,44 U/l; Kreatinkinase 1,63–559,53 U/l; γ-Glutamyltransferase 2,5–14,46 U/l; Glutamatdehydrogenase 0,68–14,78 U/l; Fruktosamin 248,08–501,43 µmol/l; Gallensäure 0,76–19,63 μmol/l; Gesamtprotein 48,66–73,64 g/l; Harnstoff 2,63–10,28 mmol/l; Kreatinin 51,38– 154,35 µmol/l; Kalzium 3,02–4,3 mmol/l; Magnesium 0,66–1,51 mmol/l; anorganisches Phosphat 0,54–2,18 mmol/l; Natrium 132,61–154,0 mmol/l und Kalium 3,52–6,04 mmol/l. Schlussfolgerungen und klinische Relevanz: Die Berechnung von Referenzwerten aus einer großen Anzahl von Routineeinsendungen ermöglicht die Etablierung von laboreigenen Referenzwerten, ohne auf Tierversuche angewiesen zu sein.

Key words

Hematology - biochemistry - Reference Limit Estimator - parameter - small mammals

Schlüsselwörter

Hämatologie - Biochemie - Reference Limit Estimator - Parameter - Klein-säuger
 

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