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CC BY-NC-ND 4.0 · Int Arch Otorhinolaryngol 2019; 23(03): e267-e275
DOI: 10.1055/s-0038-1676654
Original Research
Thieme Revinter Publicações Ltda Rio de Janeiro, Brazil

The Effects of Riluzole on Cisplatin-induced Ototoxicity

Selin Üstün Bezgin 
1   Department of Otorhinolaryngology, Kanuni Sultan Süleyman Education and Research Hospital, İstanbul, Turkey
,
Kadir Kemal Uygur
2   Department of Otorhinolaryngology, Gazi University Faculty of Medicine, Ankara, Turkey
,
Çağıl Gökdoğan
3   Department of Otorhinolaryngology, Muğla Sıtkı Koçman University Faculty of Medicine, Muğla, Turkey
,
Çiğdem Elmas
4   Department of Histology and Embryology, Gazi University Faculty of Medicine, Ankara, Turkey
,
Güleser Göktaş
5   Department of Histology and Embryology, Lokman Hekim University, Faculty of Medicine, Ankara, Turkey
› Author Affiliations
Further Information

Publication History

04 July 2018

21 October 2018

Publication Date:
01 March 2019 (online)

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Abstract

Introduction Riluzole (2-amino-6-trifluoromethoxy benzothiazole) is known as a neuroprotective, antioxidant, antiapoptotic agent. It may have beneficial effects on neuronal cell death due to cisplatin-induced ototoxicity.

Objective To evaluate the effect of riluzole on cisplatin-induced ototoxicity in guinea pigs.

Methods Twenty-four guinea pigs, studied in three groups, underwent auditory brainstem response evaluation using click and 8 kHz tone burst stimuli. Subsequently, 5 mg/kg of cisplatin were administered to all animals for 3 days intraperitoneally (i.p.) to induce ototoxicity. Half an hour prior to cisplatin, groups 1, 2 and 3 received 2 ml of saline i.p., 6 mg/kg of riluzole hydrochloride i.p., and 8 mg/kg of riluzole hydrochloride i.p., respectively, for 3 days. The auditory brainstem responses were repeated 24 hours after the last drug administration. The cochleae were analyzed by transmission electron microscopy (TEM).

Results After drug administiration, for 8,000 Hz stimulus, group 1 had significantly higher threshold shifts when compared with groups 2 (p < 0.05) and 3 (p < 0.05), and there was no significant difference in threshold shifts between groups 2 and 3 (p > 0.05). Transmission electron microscopy findings demonstrated the protective effect of riluzole on the hair cells and the stria vascularis, especially in the group treated with 8 mg/kg of riluzole hydrochloride.

Conclusion We can say that riluzole may have a protective effect on cisplatin- induced ototoxicity. However, additional studies are needed to confirm these results and the mechanisms of action of riluzole.

Keywords

riluzole - cisplatin - ototoxicity - auditory brainstem response - transmission electron microscopy

Note

This study was done in Gazi University Faculty of Medicine, Ankara, Turkey.


This study was approved and monitored by Gazi University Animal Experiments Local Ethics Committee (G. Ü. ET - B.30.2.GÜN.0.05.06.00/80-6489.


Financial Disclosure

The study was supported by a grant from the Scientific Research Projects Commission.


 

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