Comparison of the Interactions of Fibrinogen and Soluble Fibrin with Washed Rabbit Platelets Stimulated with ADP

Summary

Because fibrin, formed at a site of vessel wall injury, is involved in the formation and stabilization of a platelet aggregate or thrombus, we have studied reactions of fibrin with rabbit platelets. Gly-Pro-Arg-Pro, an inhibitor of fibrin polymerization, was used to prepare soluble fibrin. Fibrin alone did not cause aggregation of washed platelets, but addition of ADP caused aggregation and deaggregation identical to those observed in the presence of fibrinogen. Specific binding of ¹²⁵I-fibrin to ADP- stimulated platelets was similar to that of ¹²⁵I-fibrinogen, but ¹²⁵I-fibrin did not dissociate, even in the presence of high concentrations of apyrase. High non-specific binding of ¹²⁵I-fibrin was observed that was not associated with aggregation. EDTA, prostaglandin E₁ (PGE₁) and creatine phosphate/creatine phos- phokinase prevented ADP-induced aggregation in the presence of fibrin and caused rapid deaggregation when added after ADP. They also inhibited ¹²⁵I-fibrin binding when added before ADP, and EDTA or PGE₁ caused partial dissociation of bound ¹²⁵I-fibrin. In vivo, fibrin may bind to stimulated platelets, polymerize, form a gel, and interact with components of the plasma, the platelet aggregate, and the exposed subendothelium.

Keywords

Platelets - Fibrinogen - Fibrin - ADP

• References

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